Thanks for the excellent video. I have few questions 1. What can we use instead of stomacher bags ? 2. How to prepare sample if we have swab samples or environmental samples ? 3. Formula if we have n3 or n4 also ?
1. Large duran bottle 2. Dissolve the swab stick in 10ml buffer solution. After shaking and vortex, dilute if necessary. Then analyze. 3. First calculate average from 2 plates. In this way, make 2 values from 4 plates.
Would you please instruct us: The sample on each plate is 100μl(0.1ml) Do we need to multiply the final result with 10 for 1ml sample for this spread plate method ? We use 1 ml sample on each plate for pour plate method but we use 0.1 ml sample on each plate for spread plate method.
@@MicroChemsExperiments because according to the FDA-BAM Chp 1 the sample weight stated to be 50g. So I just want to ask did the result for 25g different from 50g?
But in iso method 21527-2 is written: 9.2.3 Incubate the prepared plates aerobically, lids uppermost, in an upright position in the incubator at 25 °C ± 1 °C for 5 d to 7 d. If necessary, leave the agar plates to stand in diffuse daylight for 1 d to 2 d.
I haven't received notifications for your posts in a while. I missed you, your team, and your videos! Thank you! Welcome back!
So glad to hear this. Actually we were so busy in our professional life. Now, we are back. Stay with us.
Thanks for procedure
This video's are very helpful to me
this I waited for long, thanks
I made it for you
Thank you
Good video
Thanks for the excellent video. I have few questions 1. What can we use instead of stomacher bags ? 2. How to prepare sample if we have swab samples or environmental samples ? 3. Formula if we have n3 or n4 also ?
1. Large duran bottle
2. Dissolve the swab stick in 10ml buffer solution. After shaking and vortex, dilute if necessary. Then analyze.
3. First calculate average from 2 plates. In this way, make 2 values from 4 plates.
Nice teaching mam
It's my pleasure
Excellet video
Thank you very much!
merci pour la video
Well explained thanks
Sehr gut 👍
Hi, is okay to use potato dextrose agar? with same procedure?
Yes.
thanks
You are welcome
What are the other media that can use other than DRBC agar??
You can also use "Yeast Extract Chloramphenicol Agar"
@@MicroChemsExperiments thank you
Can we use other media like Potato dextrose agar (PDA), Yeast extract Calcium carbonate agar (YECA) and Sabouraud dextrose agar (SDA)?
What is meant by BAM in the title?
Bacteriological Analytical Manual
Which temperature to set incubator 22-25° or 37° plzzz reply 😢
26 degree for mould yeast with slowly incubation 5 day
For yeast and mould 25-30 Degree Celsius for 2 days
For TPC 37 Degree Celsius for 1 day
Can we use potato Dextrose Agar instead of DRBG Agar
Yes you can but you need to add chloramphenicol as a supplement to make it more selective for Yeasts & Molds.
R can we use potato Dextrose Agar w/chloramphenicol
Hello. Does pour plating method can be used for yeast and mold culture?
In that case you have to use SDA media for pour plate
Please do the video of residue free chlorine
Noted
Can u please tell ........What is the formula for calculation if colony count in one successive dilution is less than 150?
Is this method same with ISO 16212:2017?
You said now we isolate bacteria?
Please can I use thesame technique and culture Media for pharmaceutical samples?
Yes. You can
Kindly upload the Enumeration technique of Bacillus Coagulans
Noted
Would you please instruct us:
The sample on each plate is 100μl(0.1ml)
Do we need to multiply the final
result with 10 for 1ml sample for this spread plate method ?
We use 1 ml sample on each plate for pour plate method
but we use 0.1 ml sample on each plate for spread plate method.
Do you still need to add antibiotic solution in the media?
No. No need to add antibiotics in DRBC agar media
@@MicroChemsExperiments thank youu
Hi! Great video. Just want to ask did the sample weight change from 25g to 50g?
The question is not clear.
We took 25g sample for testing
@@MicroChemsExperiments because according to the FDA-BAM Chp 1 the sample weight stated to be 50g. So I just want to ask did the result for 25g different from 50g?
@@Ninxh678 Result will not differ.
@@MicroChemsExperiments Thank you!
is there any video for bacterial count?
Yes. Link: ua-cam.com/video/IlTnLEgivts/v-deo.html
Can we use Butterfield phospate buffer instead of peptone water
Yes you can
@@MicroChemsExperiments thank you ❤
Can we use this method for determination of yeast and mold?
Yes
For enumeration need 6 falcon tube then how about determination do we still use 6 falcon tube or just use 2 tube only ?
@@sambathsocheata1389 you can use only 2 tubes. It depends on the expected concentration of yeast and mold in your sample
Thank you ❤
Can we use blending jar instead of stomacher machine
Contamination can occur in blender jar. So you can shake by your hand....or you can use vortex mixer
@@MicroChemsExperiments thank you
@@gousiagulzar7002 welcome
Why we use diluent instead of water
Please upload video for Staphylococcus aureus and Enterobacteriaceae count
Noted
👍
Thanks
Is there any chemicals to prevent yeast and mould in packeged drinking water
Yes. benzoic acid, sodium benzoate, propionic acid, sorbic acid, and sodium diacetate are used to prevent the growth of yeast and mould
Excuse me, can I get the reference of calculating formula?
Reference is given in the video title
@@MicroChemsExperiments Many thanks to you. Keep this good work always!☺️
@@sorngsinadane8447 Thank you so much.
Can you use buffered peptone water instead of 0.1% peptone water?
You can
Incubate plates in upright or invert position
Invert
But in iso method 21527-2 is written:
9.2.3 Incubate the prepared plates aerobically, lids uppermost, in an upright position in the incubator
at 25 °C ± 1 °C for 5 d to 7 d. If necessary, leave the agar plates to stand in diffuse daylight for 1 d to 2 d.
How to control yeast and mould in packeged drinking water
Add one of these chemicals benzoic acid, sodium benzoate, propionic acid, sorbic acid, and sodium diacetate.
@@MicroChemsExperiments thank you for your kind reply one more doubt sir how much qty add per litre sir
Hi, I am wondering what is the purpose of labeling 2 trial plats? eg: DRBC10-2 / trail -1 and DRBC10-2 /trail -2.
Thanks in advance.
Contamination can occur in every steps of any microbiological test. So double trial can increase your confidence level of the test.
what is the different about bacteria and yeast in Macroscopic ? I am still confused. Thank you
Study more in Google resources
I haven't received any reply
Sorry for late reply
one more issue media are not solidified
Which temperature to set incubator 22-25° or 37° plzzz reply 😢
Which temperature to set incubator 22-25° or 37° plzzz reply 😢
37
Which temperature to set incubator 22-25° or 37° plzzz reply 😢