I'm a farmer not a trained scientist But I wanted to check my own cfu on the biological products I buy. This video is the best step y step. Thanks a lot for shearing your pedagogical skills
Dear Friends Thank you so much for watching @biologyexams4u Please consider subscribing using the link: bit.ly/3kG2kKf A simplified video explaining to calculate cfu/ml of original stock? Hope this will help Watch, Understand and enjoy Biology :)
Thank you so much. I just couldn't figure this out with the materials provided to me by my professors. It's so hard sometimes to figure out these things out for yourself, when the given material is always in a foreign language and has many terms, which you only are familiar with half at the best circumstances..
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Very helpful, thank you for making this video, i have a question, sir what if we have 2 plate for each dilution (duplo) how to calculate ? Example: 10^-3 10^-4 Petri 1 287 31 Petri 2 292 42 Vol of stock transferred = 0.1 ml Thank you, sir
Your videos are excellent, if possible please make some videos on PCR, Elisa, buffer preparation, sds-page, electrophoresis, endotoxin test and their calculations.
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alternatively, couldn't you use: no. of colonies/ TDF x Volume of culture in mL? As long as the units are consistent between DF and Volume and multiply those two values and then divide that by the colony value
Thank you so much you explained it very well 1st time I tolerate it ^_^ Please I have a Q, might be silly. now after counting it plate, tube (f) looks has low count, but after calculation it will be 10*8 in compared to tube (e). Does it mean higher count (f)? .
Welcome.. Thank you for the question. We often wont consider the plate with low count or high count as the chance of error is high. Hope I answered the question.
Hi Mandeep Phosphate buffered solution or isotonic saline solution (0.85%)is commonly used as both wont causes osmotic shock..other diluents is Sterile distilled water . Often the selection of diluents depends on the further procedure and also the type of microorganisms ... Like for cell count or for subculturing.. Thank you
if you diluted 1ml of a sample with 99ml of sterile water then plated 100ml of the diluted sample on plate count agar and 75 colonies were found. calculate the cfu/ml of the original undiluted sample can you please help with this
Really appreciate your question.. Here we are using dilution factor not dilution.. Hope this video on dilution factor will help ua-cam.com/video/EOsYOfKMgS8/v-deo.html Thank you so much 👍
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I'm a farmer not a trained scientist But I wanted to check my own cfu on the biological products I buy. This video is the best step y step. Thanks a lot for shearing your pedagogical skills
This is the best crash course I could find. Thank you so much!
It has never been made this easy before. Thank you.
Dear Friends
Thank you so much for watching @biologyexams4u
Please consider subscribing using the link: bit.ly/3kG2kKf
A simplified video explaining to calculate cfu/ml of original stock?
Hope this will help
Watch, Understand and enjoy Biology :)
I love how concise and to the point this explanation is. Helped me a bunch!
Thanks 🎉 you saved me an hour before my Microbiology test 😊 You are my G.O.A.T
Thank you so much. I just couldn't figure this out with the materials provided to me by my professors. It's so hard sometimes to figure out these things out for yourself, when the given material is always in a foreign language and has many terms, which you only are familiar with half at the best circumstances..
Glad I could help! Thank you so much for your nice words.
Thank you so much! I'm taking A-level Biology and you explained it so well :)
Really happy that the video helped. Thank you so much
thank you so much Sir. this is very helpful in doing the research i'm doing right now
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A very awareness explanation by good manners 😊
Thanks a lot
🙏
Welcome Rezvan...Thank you:)
Thank you so much. This video was so helpful!
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Thank you so much ❤!
Thank you very much greatly helpful
You're welcome!
Just sharing our Microbiology playlist for your reference.
ua-cam.com/play/PLpKNQ2U3np9i5tZ5kNNZ-yAGWAHxvG5j1.html
Stay blessed. Thank you so much @biologyexams4u
Life saver ❤
Nice explanation
Very well explained
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All the best
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Nicely explained. Thank you
This is a nice video. well explained. Thank you sir for the video.
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Thank you for your nice words
Please consider subscribing and sharing with your friends
Thank you so much:)
Very helpful, thank you for making this video, i have a question, sir
what if we have 2 plate for each dilution (duplo) how to calculate ?
Example:
10^-3 10^-4
Petri 1 287 31
Petri 2 292 42
Vol of stock transferred = 0.1 ml
Thank you, sir
Take the average
Great
Great presentation. Why we took 0.1 ml and how we can take such minute quantity.
Small quantity; there wont be overcrowding of bacterial cell. We can take small amount using micropipettes. thank you
You can use calibrate your micropipette at 10 microliters (=0.1 ml)
Hello sir
Thank
My question is
Sample of E. Colo it is use one loopful cuture on the plate
Thanks times 10 raised to 10000000
Thank you so much. really appreciate your creativity. happy to know the video helped. Pinning this comment. take care, stay blessed
Your videos are excellent, if possible please make some videos on PCR, Elisa, buffer preparation, sds-page, electrophoresis, endotoxin test and their calculations.
Noted.
Our channel has crossed 50K subscribers with Almightys grace and your support.
⏬ Download free E Book: Introduction to Recombinant DNA Technology www.biologyexams4u.com/2022/09/enroll-now-new-online-course-on.html
📢Enroll Now Free Certificate course on Introduction to Recombinant DNA Technology alison.com/course/introduction-to-recombinant-dna-technology?
Thank you so much:) @biologyexams4u
alternatively, couldn't you use: no. of colonies/ TDF x Volume of culture in mL? As long as the units are consistent between DF and Volume and multiply those two values and then divide that by the colony value
Do i have to count for the overall average for the three plates or i shouldve just ignore the ones tgat are not in range?
Thank you so much
you explained it very well 1st time I tolerate it ^_^
Please I have a Q, might be silly. now after counting it plate, tube (f) looks has low count, but after calculation it will be 10*8 in compared to tube (e). Does it mean higher count (f)?
.
Welcome.. Thank you for the question. We often wont consider the plate with low count or high count as the chance of error is high. Hope I answered the question.
Hello, could pls help me, I ,0.5ul of stock solution diluted in 495.5ul of diluent what is the dilution factor
Thank you
hi, If we use raw samples there will be have dilution factor. Also how to count for raw samples transfer into plate 0.1ml.
First E. Coli sample O.D is what we consider?
Hello Sir, I've a question. How to prepare 4×10⁸cfu/ml ? An earliest response will be immensely valuable.
How to get volume of culture plate
Nice
Dilution will be done by adding media or distill water or buffer
Hi Mandeep
Phosphate buffered solution or isotonic saline solution (0.85%)is commonly used as both wont causes osmotic shock..other diluents is Sterile distilled water . Often the selection of diluents depends on the further procedure and also the type of microorganisms ... Like for cell count or for subculturing..
Thank you
What is the formula if we get countable colonies in two plates ?
if you diluted 1ml of a sample with 99ml of sterile water then plated 100ml of the diluted sample on plate count agar and 75 colonies were found. calculate the cfu/ml of the original undiluted sample can you please help with this
What if all of them are within range, will you find the CFU/ml of each and then find the average?
How do you get ten into the plus numbers? The calculations gives 10 to the (-) values Right?
Hi Sajith
We are using dilution factor not dilution.. Hope this video will help ua-cam.com/video/EOsYOfKMgS8/v-deo.html
Thank you so much
@@biologyexams4u OK. Thank you so much for understanding me. Got it.
how is the voulme plated i.e 0.1 ml is calculated?
What if I want to have a bacterial solution with 1*10^7 for example. How do I have to prepare it with this 6.3*10^7 solution?
From the 6.3 x 10^7, how to make it 10^6 bacterial suspension?
how can we get 10^7 as final when we had 10^6 after 10^5/0.1?
Sir how to calculate aliquot factor in endophytic analysis??
i have a broth culture of a bacteria and i need 10^9 CFU/ml concentration of it to inoculate in a material. Can you help me how i can do that?
What is your colony count? Did you do serial dilution and plated on an agar plate?
Volume of culture plated should be 1 ml
This is the standard procedure as per the manual. There may be change in volume.. Yes... Thank you
How do we know the number of colonies?
Using bacterial colony counter...
Thank you
How did you get 443, 63, and 3 colonies?
Why is vol. of culture plated 0.1 ml and not 1 ml as you use 1 ml from the total 10 ml?
If 1 ml is used there is always a chance of overcrowded plates
@@biologyexams4u then the 63.10^6 CFU should be in 0.1 mL not 1 mL and afterward express it in CFU/ mL
Total dilution factor ..? How to calculate that ?
Hi hope this will be helpful in understanding dilution factor ua-cam.com/video/EOsYOfKMgS8/v-deo.html
Serial dilution se
Didn't get the 0.1ml part. Could anyone explain?
c'est quoi la différence entre ufc/ml et ufc/g ? quand on doit calculer par ml et quand on doit calculer par gramme ?
not understanding how it went from 10 to the 6th power to 10 to the 7th power though can someone explain?
Hi Sharisa Lee
Which part? Is it the final calculation? 63X10*6 and 6.3x10*7 both values are same.
yes, you were at 10 *6 but final answer became 10*7 so I'm not sure how final answer became 10*7
is it because once you move the decimal over to the left to get 6.3 instead of 63 , the 10*6 changes to 10*7 ?
what is the difference between ufc/ml and ufc/g ?
ML for when we use a liquid sample (eg milk)
G for when we use a solid sample (eg a sandwich)
Someone give the value of total number of cells
83x 10 power 8.
Is it possible.. please clear calculation if possible
Hindi language me video banate to pura samj aata
Sir why we don't consider minus sign of dilution when doing calculations ?
Really appreciate your question.. Here we are using dilution factor not dilution.. Hope this video on dilution factor will help ua-cam.com/video/EOsYOfKMgS8/v-deo.html
Thank you so much 👍
Thank you!❤
Thank you Sir.
Thank you sir.
Thank you soo much
Thank you for this