Edman degradation | Edman Sequencing

Edman degradation is a a method used for the sequencing of peptide/protein. In this method the peptide is allowed to react with edmans reagent under mild alkaline conditions. The edmans reagent reacts with
the N terminal amino acid and forms a complex. This complex is then cleaved from the peptide and separated by chromatography or electrophoresis. The method can be repeated several times to find the sequence of the peptide.

[@eldritchelsbells]

Awesome video! Short and efficient

[@electronic5metallica]

Short and sweet, thanks!

[@nimramukthar1721]

brilliant way of teaching. Thank you Sir.

[@sampathkumar9379]

very good explanation i loved it

[@risharajkhowa5060]

Nice,very good explanation

[@laravasconcelos6024]

Thanks for share! Great explanation

[@jesusvalencia5520]

Increíble videooo. Se entiende perfectamente. Muchas gracias!!

[@garimasetia268]

please make a video on illumina sequencing

[@momndaughterdiaries8453]

Superb video 👍❤️

[@as-ed7fq]

Thanks so much for your help

[@alissonandradealmeida5195]

Great content. Thank you for share

[@10vtannap]

Thank You u explain well

[@sangitamandal6146]

You are great sir❤

[@antoineloth8196]

Merci beaucoup !

[@alexisvorstman125]

you are my hero

[@JosePH-mr6dg]

Thank you 🌹 so much

[@rauljimenez2813]

thank you man

[@mayuribirajdar3215]

Thanks ❤️

[@brijeshk.swarnkar01]

Short and efficient

[@izzadina5951]

Thanks alot

[@nishalkhan6264]

thank u ...sir keep it up...

[@Farhan-co9he]

very good bro

[@mariapaulacadenamalaver4727]

Why is it that you can do only upo to 30 aminoacids?

[@Rahmath_Poovancheril]

Thanks

[@productdeals4513]

After N -terminal amino acid is detached why not next amino acid also react with pitc at the same time ?

[@laravasconcelos6024]

I think that is possible because of the change in the pH conditions. The aminoacid is labeled in the alkaline media, and just cleaved in the acid.

[@hbacademyofscience]

best of bests

[@humnarafique6693]

Great 😊❤️

[@jhrvta]

Oh my goodness! I wonder how many people watching this has even used (or maintained) one of these beasts? I love the smell of R2 in the morning... it smells like victory!

[@faisalkhanzarrar2718]

it is good

[@xHarionago]

long live india

[@ramankumarlaldas8697]

Moye moye

[@xHarionago]

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[@aradhanakaushik24]

😂😂🎉

[@edmanjoshelguzmanandres1546]

🙉

[@kaladharyadhav7697]

Hill
This Indian.