Salting in and salting out | How does salting out happen? | what is salting in of proteins?

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  • Опубліковано 28 січ 2025

КОМЕНТАРІ • 459

  • @ranjitshaw9110
    @ranjitshaw9110 3 роки тому +49

    This is the best video for explaining the concept of Salting in and Salting out.... Thankyou dada for this....l would like to ask you about the reference where l can get the exact text of this explanation!!!!!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +2

      There is no 1 proper source for it ....several review articles and text books . But you can follow voet and voet

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +3

      Please share my channel link with your friends and help me to reach big audience

    • @ranjitshaw9110
      @ranjitshaw9110 3 роки тому +2

      @@animatedbiologywitharpan Ok dada....l will try to look into them

    • @ranjitshaw9110
      @ranjitshaw9110 3 роки тому +1

      @@animatedbiologywitharpan Sure

    • @ranjitshaw9110
      @ranjitshaw9110 3 роки тому +1

      Is there any video of Parkinson's disease made by you?

  • @martaromeromarcos5816
    @martaromeromarcos5816 4 роки тому +13

    our teacher's explaining his subject with ur video, you can be proud bro

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому +1

      Marta Romero Marcos please share my channel link with your friends and help me to reach big audience

    • @marcosmartinandres710
      @marcosmartinandres710 4 роки тому

      @@animatedbiologywitharpan She shared it with me!!! I've learnt a lot!

  • @shawnmakiri4435
    @shawnmakiri4435 4 роки тому +35

    Thank you. You did something my textbook and my lecturer couldn't do.

    • @skydivenext
      @skydivenext 9 місяців тому

      no is because is very boring why do I have to study stupid biochemistry for my degre what a joke bro lol

  • @jenniejean-baptiste9467
    @jenniejean-baptiste9467 2 роки тому +2

    I usually have a hard time understanding how some of these processes work but you did an amazing job at explaining the process! I feel like I understand it and could explain how it works to another individual now. Thank you!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

  • @gigiarias7659
    @gigiarias7659 4 роки тому +7

    Such a great refresher a year after my biochemistry courses! The graphics are really helpful, great work!

  • @pawanrohilla01
    @pawanrohilla01 5 років тому +2

    You are a ray of hope for all of us aspirants like me 🇮🇳🇮🇳

  • @calebsammons9341
    @calebsammons9341 3 місяці тому +1

    This video really made this topic make a lot of sense when my textbook left me confused! Thank you!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 місяці тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @ria7657
    @ria7657 5 років тому +3

    finally it's crystal clear to me...thank you so much!

  • @danahhamad8088
    @danahhamad8088 3 роки тому +4

    I was struggling with this concept but you sir explained it very well. Thank you 🙏🏻

  • @millykunda4537
    @millykunda4537 3 роки тому +3

    You are legit making biochemistry easy for me 🙌

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Please share my channel link with your friends and help me to reach big audience

  • @argowal
    @argowal 4 роки тому +9

    Great work, thank you for your presentation, it was clear and informative. the use of graphics greatly helps, keep it up! Would love more about protein separation and electric properties.

  • @rebeccaschill4880
    @rebeccaschill4880 2 роки тому +2

    BLESS U BRO ,SERIOUSLY 🥺😭u saved my ass in 12 hours i have my exam and this made me finally understand
    💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Share my channel link with your friends and help me as well

  • @aishwaryas4696
    @aishwaryas4696 3 роки тому +2

    You saved my day within 6 mins🤗 Thank you!

  • @SuperPuddingFish
    @SuperPuddingFish 4 роки тому +4

    Short and clear, thank you!

  • @rayaroy519
    @rayaroy519 4 роки тому +2

    Thank you for this lecture video. I have never understood this topic before and the mechanism behind it. This video has done it for me.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому

      Do explore other videos I. My channel.... if you like my content please share among your friends

  • @asylahblairewahing198
    @asylahblairewahing198 Рік тому +1

    Thank you for this. Was able to understand it better thanks to this video.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому

      Please share my channel link with your friends and help me to reach big audiance namaste 🙏

  • @wahidahyusof4299
    @wahidahyusof4299 2 роки тому +1

    Best video ever and very easy to understand! i literally understood everything in 6 mins thank you so much! you are an amazing teacher!!

  • @megnareddy9499
    @megnareddy9499 4 роки тому +2

    Thank you!!! This video was very well explained and helped me understand something even my professor could not help with

  • @swatisrivastva4440
    @swatisrivastva4440 3 роки тому +1

    Less time with clear concept great 😊 thanks for this 👍

  • @shirharel6520
    @shirharel6520 2 роки тому +1

    1 hour of lecture in 6 minutes... thanks!! :)

  • @pratibhamishra5370
    @pratibhamishra5370 4 роки тому +1

    Thanks you so much dear , I have finally clear my concept with this video . Thanks to you once again .

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому

      Please share my channel link with your friends and help me to reach big audience

    • @pratibhamishra5370
      @pratibhamishra5370 4 роки тому

      @@animatedbiologywitharpan sir please make a video on water and their bond forming mechanism with biomolecules . Please sir this mine humble request to you.

  • @조하성-p2d
    @조하성-p2d Рік тому +1

    Best explanation for salting in and out. I want to cite this reference
    Haha. Thank you so much

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому +1

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @조하성-p2d
      @조하성-p2d Рік тому

      ​@animatedbiologywitharpan
      I am really interested in your video!
      Could you let me know more about the detailed mechanism of the Salting-in step? From your video, I understand that adding salt ions can make the structure of the protein stable along with reducing of the intermolecular replusion of the protein. Then, what is the mechanism of the water molecular can form the hydrate shell around by the protein?
      Is hydrogen bonding on the hydrophilic regions of the protein?
      Thank you!!!!

  • @crystal_xxi
    @crystal_xxi 2 роки тому +1

    FINALLY, I understood this. Thanks so much!

  • @simonclarke2276
    @simonclarke2276 2 роки тому +1

    Thank you - very clear and concise explanation!!

  • @dcroko
    @dcroko Рік тому +1

    Awesome explanation. You did a finer job at explaining this concept than my professor. Thanks again.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому

      Please share with friends. Please subscribe to my second channel. youtube.com/@letscrackbioexams94?si=ZWdPXv2NSpZeDHZu

  • @primordial7305
    @primordial7305 3 роки тому +1

    I love how you teach it. Even a dummy like me can go through medical school just by watching your tutorials.

  • @krupanidhi912
    @krupanidhi912 9 місяців тому +1

    Ammonium sulphate precipitation follows the procedure explained by you. It is the good and simple method instead of ultracentrifugation and other methods. Nicely explained. Continue.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  9 місяців тому

      Could you please help me by sharing my contents with your students . I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @kantamaniveeravenkatanaray232
      @kantamaniveeravenkatanaray232 8 місяців тому

      Please requesting you send the procedure to me

  • @subbud2120
    @subbud2120 5 місяців тому +1

    I can understood so easily, Very good explanation sir

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  5 місяців тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @a3ministry997
    @a3ministry997 Рік тому +1

    Thank you ! The concept is clear for me now😊

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому

      Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.

  • @uvy3908
    @uvy3908 4 роки тому +1

    I like your animations it helps give a clearer pictuce

  • @MariaChavez-ey5sr
    @MariaChavez-ey5sr Рік тому +1

    Our group just used your video and it was very informative about explaining the topic. Thank you!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому

      Thanks a lot, please support our channel by super thanks. Your 1$ contribution is my motivation.

  • @pieromg8587
    @pieromg8587 Рік тому +2

    Thank you so much for making this fantastic video ❤

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому

      Please support me by subscribing to mu second channel youtube.com/@letscrackbioexams94?si=l2Di6Uc4hCYYa3ve

  • @shalaka6502
    @shalaka6502 3 роки тому +1

    Thanks alot for the video my concepts are Cleared very well.

  • @khadijakhan5290
    @khadijakhan5290 2 роки тому +1

    Very informative. Quick and easy to understand. Animation make it more clear.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
      I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
      You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

  • @ahmad43754
    @ahmad43754 4 роки тому +1

    You make it easier than I thought, thanks a lot dude.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому

      Please share among your friends and help me to reach big audience

    • @ahmad43754
      @ahmad43754 4 роки тому +1

      @@animatedbiologywitharpan one of my friends share it with us in the group 😭❤️

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому

      Ahmad Abdullrahman check out all of my playlists....might be useful

  • @AmruMagdy
    @AmruMagdy 9 місяців тому +1

    Jazak Allah khyra sir

  • @susuyao2654
    @susuyao2654 3 роки тому +1

    Thank you for explaining this concept clearly!!!

  • @gisellvm3170
    @gisellvm3170 2 роки тому +1

    Very good explanation.Thank you very much!!!!!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

  • @Hiiraatif
    @Hiiraatif 3 роки тому +2

    it was an awesome video. you explained so well. thank you soooooo much

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Please share my channel link with your friends and help me to reach big audience.

  • @strikefreedom7575
    @strikefreedom7575 4 роки тому +7

    Helps a lot! Thanks!

  • @roser8325
    @roser8325 4 роки тому +1

    Thank you for this video it was explained very well. Got in on the first take!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому

      Glad to know that it was helpful. Please share my channel link with your friends and help me to reach big audience

  • @abhijeetsurwase9665
    @abhijeetsurwase9665 2 роки тому +1

    Really useful video. Such a tricky concept made like easiest thank you 👌🏻

  • @mariamkhan1735
    @mariamkhan1735 2 роки тому +1

    Thank you! This helped a lot! I really appreciate your videos.

  • @mlawliet6151
    @mlawliet6151 3 роки тому +2

    Tysm, this was really useful and informative for me. I’ve just had a lecture about protein separation at the university and got confused but your animation helped me so much

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Please share my channel link with your friends and help me to reach big audience

  • @anzosimon19
    @anzosimon19 Рік тому +1

    Concept clearly explained❤

  • @lukachinyemba5619
    @lukachinyemba5619 Рік тому +1

    Wonderful 💯💯

  • @barathenpillai5497
    @barathenpillai5497 4 роки тому +1

    what a legend. explained better than my uni

  • @rajneeshkumar_2067
    @rajneeshkumar_2067 4 роки тому +1

    Literally bro.. Yor presentation is too good!! Thanku 👌👌👌

  • @joeljamesdn
    @joeljamesdn 3 роки тому +1

    Thanks! It was clear and easy to follow.

  • @onashadissanayake2354
    @onashadissanayake2354 3 роки тому +1

    it was very informative and very clear. Thank you so much

  • @subhabratpanda3673
    @subhabratpanda3673 4 місяці тому +1

    Excellent video..m

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 місяці тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @almaperez8342
    @almaperez8342 3 роки тому +1

    Clear and amazing explanation. Keep up the good work!!

  • @hmh6040
    @hmh6040 2 роки тому +1

    Thank you for your such nice and clear cartoon!

  • @ducktape8520
    @ducktape8520 11 місяців тому +1

    Great video! Just have a litte remark:
    At 1:52 you mark the carboxylate ion which is part of the protein peptide backbone. Instead you should have marked the carboxylate at the amino acid side chain which is exposed to the enviroment.

  • @medic_divers11
    @medic_divers11 3 роки тому +1

    full of knowledge

  • @shreyaroy5588
    @shreyaroy5588 4 роки тому +1

    very nice and point wise explination.Thank you a lot.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому +1

      Please share my channel link with your friends and help me to reach big audience

  • @LeonEllisZ
    @LeonEllisZ 2 роки тому +1

    This makes so much sense! Thank you.

  • @aileneroque8320
    @aileneroque8320 7 місяців тому +1

    I understand salting in and salting out based on their definitions, but I don't understand why at 1:33 the definitions are placed on either side of the graph like they are? I would assume salting in would be in the left side, and salting out on the right?
    Edit:
    There is a clearer graph at 4:53 with the definitions on the "correct" side.

  • @malva_fiore
    @malva_fiore 2 роки тому

    Explained in easy way, with drawings - the best videos 😍 thank you for your work

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому +1

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

  • @MehediHasan-zc9dh
    @MehediHasan-zc9dh 3 роки тому +1

    It was very helpful and easy. Thanks

  • @edvaldomaciel510
    @edvaldomaciel510 2 роки тому +1

    Thank you so much for the explanation 🙏. Your wise way to explain alongside the illustration helped me in clarifying some points about the topic.

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Please share my channel link with your friends and help me reach out to big audience

  • @antoniomele436
    @antoniomele436 4 роки тому +1

    This video was very helpful! Thank you! Keep up the excellent work!

  • @Org2529
    @Org2529 4 роки тому +1

    Finally i understood..thanks
    😅

  • @zibamajidi3026
    @zibamajidi3026 4 місяці тому +1

    very nice and simple, thank you

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 місяці тому

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @amairaniramirez8855
    @amairaniramirez8855 4 роки тому +1

    Thank you so much! Your explanation was very clear!

  • @leonnemulder549
    @leonnemulder549 4 роки тому +1

    Great video short and very clear!!!!!

  • @abcsin6526
    @abcsin6526 4 роки тому +1

    Excellent

  • @sarairamirez9272
    @sarairamirez9272 4 роки тому +1

    best explanation ever!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому

      ua-cam.com/channels/4IpyopsGWSjaPACNTZLuqg.html please subscribe to my second channel as well

  • @thaynavasconcelos803
    @thaynavasconcelos803 Рік тому

    A really nice explanation. A hug from Brasil!

  • @bradding578
    @bradding578 7 місяців тому +1

    great and clear explanation

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  7 місяців тому +1

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

    • @Nargis.1212
      @Nargis.1212 5 місяців тому

      @@animatedbiologywitharpan I just wanted to take a moment to appreciate your incredible work. Your unique teaching style is truly refreshing and inspiring. You have a remarkable ability to present complex topics in such an engaging and accessible way. Your creativity and passion for educating others shine through in every video. Thank you for making learning enjoyable and for sharing your expertise with us.

  • @Gerolinger
    @Gerolinger 2 роки тому +1

    What a great video. Thank you very much

  • @alandbestwn1055
    @alandbestwn1055 2 роки тому +1

    Thank you,You understood me very well،Keep making more videos🌹🤍

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Small contribution like $5 means a lot for us.

  • @linaeline
    @linaeline 2 роки тому +1

    Thank you YOU'RE THE BEST👍

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance

  • @NaderSharif-p8p
    @NaderSharif-p8p 6 місяців тому +1

    Great work buddy keep this up

  • @trisitachowdhury8089
    @trisitachowdhury8089 4 роки тому +1

    Great video।।

  • @aneeshrisbud347
    @aneeshrisbud347 2 роки тому +1

    Great Animations

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  2 роки тому

      ua-cam.com/video/gCSKpNdJkZo/v-deo.html please like this video to support me in a global video making contest (like = nomination )

  • @amansajjad11
    @amansajjad11 Рік тому +1

    Good👏👏

  • @TanWeiJunDA
    @TanWeiJunDA 4 роки тому +1

    nice explanation, thank you for explanation its very informative and helpful

    • @TanWeiJunDA
      @TanWeiJunDA 4 роки тому

      however i have a question, regarding the protein isolation, if i not sure about the solubility strength of my desire protein, then salting in (addding low concentration of salt solution to allow it to solube is better) or salting out is better. And base on explanation, i understand that both salting in and out is helping to isolate, is there any condition like protein that not likely to soluble will be favor for salting out process? if there is, is the example fibrous protein will be more easier to seperate in salting out or salting process? Thank you for answering, look foward to knowing more on this

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому

      Please share my channel link with your friends and help me to reach big audience

  • @besttricksinfootball9146
    @besttricksinfootball9146 2 роки тому +1

    I appreciate your work sir thanks 😊

  • @aninditakumar3505
    @aninditakumar3505 3 роки тому +1

    The video is really informative. 👍

  • @rekhag-8572
    @rekhag-8572 2 роки тому +1

    Amazing videoooo

  • @walakirajude3529
    @walakirajude3529 3 роки тому +1

    The video is useful, thank you

  • @bhavishapatel8459
    @bhavishapatel8459 3 роки тому +1

    Excellent video 😃

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  3 роки тому +1

      Please share my channel link with your friends and help me to reach big audience

  • @nikitanair6219
    @nikitanair6219 4 роки тому +1

    Wow well explained ♥️

  • @ks99911
    @ks99911 4 роки тому +1

    Thankyou sir crystal clear concept 👍🙏

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому +1

      Please share my channel link with your friends and help me to reach big audiance

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому +1

      Also check out my profile in unacademy app there you would get more than 100 free of cost lectures and practice sessions

    • @ks99911
      @ks99911 4 роки тому

      Yes yes I have already viewed many of your free lectures, very helpful thankyou so much.🙏

  • @hodahabbal4845
    @hodahabbal4845 4 роки тому +1

    Thank you .. very very usefull

  • @qp5337
    @qp5337 3 роки тому +1

    This was helpful, thank you

  • @yasminvie99
    @yasminvie99 8 місяців тому +1

    Amazing video!!!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  8 місяців тому +1

      Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

  • @meghapatil9750
    @meghapatil9750 4 роки тому +1

    Well explained sir

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому

      Please share my channel link in your college group and help me to reach big audience

  • @sakshishukla03
    @sakshishukla03 3 роки тому +1

    Thank you very much
    This was very helpful 👍

  • @shravanirawool75
    @shravanirawool75 3 роки тому +1

    Great

  • @YaqeenAlalawi-v7k
    @YaqeenAlalawi-v7k Рік тому +1

    Nice explanation!👏

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  Рік тому

      Please support my channel via super thanks. Your small contribution like 1$ means a lot for us. The super thanks option is present at the lower right corner of any video, a heart sign with $ in it.

  • @salsal7309
    @salsal7309 4 роки тому +1

    Nice job

  • @aq0511
    @aq0511 3 роки тому +1

    Very helpful! Thank you!

  • @Anci3ntDice
    @Anci3ntDice 4 роки тому +1

    Very useful thank you

  • @137bob3d
    @137bob3d 3 роки тому +1

    you done good

  • @rforrebel2687
    @rforrebel2687 3 роки тому +1

    It was awesome...

  • @saharr8723
    @saharr8723 4 роки тому +1

    so helpful and so concise, thanks so much!

    • @animatedbiologywitharpan
      @animatedbiologywitharpan  4 роки тому +1

      Please share my channel link with your friends and help me reach out to big audience

    • @saharr8723
      @saharr8723 4 роки тому

      @@animatedbiologywitharpan for sure :)

  • @ET-ht4zi
    @ET-ht4zi Рік тому +1

    very good ...thank you

  • @Gabriela79830
    @Gabriela79830 3 роки тому +1

    Thank you for this video!