Building phylogenetic tree with Bootstrap value, Intra& Interspecific diversity analysis using MEGA

In this video how different phylogenetic trees are build or generated using MEGA software has been discussed from very scratch. The concept of bootstrap and how it is assessed has also been covered. Interspecific and Intraspecific distance calculation is a very important aspect of genetic diversity studies. How those distances can be calculated has also been explained in the video.
Links to the other videos of this topic -
• Download and Install g...
• How to trim and align ...
• Selection of Algorithm...
#Phylogenetics #Geneticdiversity #MEGA

[@jensonvictor84]

Awesome informative videos... Thank you.

[@saymonakter7404]

Really,this vedio is included with massive information about phylogenetic tree.Thank you so much sir, for such kind of eliciting vedio.

[@amazai]

Awesome lecture, thanks Prof.

[@snehapodder9606]

Thank you so much sir, for the detailed explanation! This was the best video on MEGA.
Can you please create a video for when to use which method for constructing a phylogenetic tree

[@mainukalita3784]

Sir first of all thank u so much for this video.so grateful to come across this.i have been looking for videos to interpret the phylogenetic trees done on megax.please make more of such videos & explain nicely.one suggestion sir if u can a little on the language,may be prepare a script so that everything becomes more clearer.
Thanks again sir.u have no idea how much this has helped me.
Please make videos on how to interpret ncbi blast sequences alignment tables also.great work.good wishes

[@ademahendra-r9r]

Is there any interpreting of different color in protein code?example, yellow color in protein A and V, what the meaning?

[@PriyankaSingh-ft9pu]

Informative video Sir!✨
Thank you for uploading such useful contents! Now everyone can use MEGA software easily✨

[@MdAlam-z5d]

Sir I am struggling with genetic distance in my thesis.
If you don't mind could you pleass see this paper on Google scholar or researchgate named "Genetic Diversity of Native Buffalo Populations in Vietnam Based on Mitochondrial D-Loop Nucleotide Sequence"
In this paper how they made table no-5 ??

[@nasrullah320]

Very Nice Sir, U Explained all in a very lucid manner
Thanks ❣️

[@NDG_Biotechie]

Thanks and welcome

[@truesherlee]

Thank you, Sir
Very helpful 👌🏽 ☺️ 🙏🏼

[@jyoti2446]

I can't thank enough...🙏🙏🙏🙏. ...very helpful video sir.

[@NDG_Biotechie]

So nice of you

[@NavonilDeSarkar]

Q1. Please tell me more about FASTA format. I also want to know more about the limit of this method. Can I use a sequences larger than ~1kb? is there a limit of sequence length?

[@NDG_Biotechie]

FASTA format is basically a text-based format, and it's used for representing either nucleotide sequences (DNA/RNA) or peptide sequences (Proteins). FASTA is the most common format for downloading sequences from the NCBI database and maximum entry-level bioinformatics software use data in this format. Now coming to your second question, I personally have used approx 1.5 kb sequences for analysis, but I am not aware of the sequence length limit.

[@kushankursarkar6222]

Sir how can i construct a phylogenetic tree from phytochemicals or pubchem id?

[@ibnudarda23]

Sir, for trimming msa. What exactly the rules for best trimming? So we dont loose alot of informations. Thanks you sir

[@NDG_Biotechie]

1. Don't trim until its unavoidable. If you watch the full video, you will see that I have mentioned if only 1 or 2 sequence of same or related family is ruining the MSA, omit them rather than trimming.
2. If your sample size is too large with varied nucleotide length, then you can trim the start and end few sequences so that all the sequences are of approximately same length, which otherwise can hamper the robustness of the tree

[@aseukaryotic9982]

When creating a phylogenetic tree with MEGA, there's an option for "Complete deletion" in the Gaps/Missing Data Treatment. If we are going to choose Complete Deletion, is it okay if I don't manually delete the gaps in my multiple sequence alignment?

[@zoubei441]

This is so useful, thx so much

[@Csr535]

Excellent explanation sir 👌

[@AparnaSuresh-l3n]

Thankyou so much sir❤

[@estherswamidason]

thank you! this was helpful

[@NDG_Biotechie]

Glad it was helpful!

[@biswajitkhan4203]

Sir please help me, how to add broot strap value in tree?

[@NDG_Biotechie]

You have to select bootstrap option before you start making the tree

[@tanzeemazeeza.s9589]

Sir could you please explain about interpreting phylogenetic tree

[@NDG_Biotechie]

Yes I am planning to do a separate video on that

[@rhythmicworld3427]

Very good

[@pradeeppenumaduofficial916]

👌👌👏👏💐💐