Yanyi Huang - Fluorogenic DNA Sequencing

Professor Huang shared several newly developed sequencing technologies, including a spatial transcriptomic profiling method called SPRINTseq, as well as fluorogenic error-correction code sequencing and fuzzy sequencing strategies for high-throughput sequencing instrumentation and applications. SPRINT-Seq, developed by Huang and collaborators, provides rapid sequencing speed and low cost. The technology combined the reverse-terminator sequencing-by-synthesis chemistry and in-situ rolling circle amplification, further coupled with a hybrid block coding strategy to solve the polyclonal crowdedness problem. Through fluorogenic sequencing chemistry, the sequencing accuracy was significantly increased by taking advantage of the information redundancy of multiple rounds of degenerated sequences for error detection and correction. Fuzzy sequencing, using only one round of degenerated sequencing, achieved a higher information efficiency of the reaction and a higher speed for re-sequencing applications.