Immunoprecipitation
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- Опубліковано 21 жов 2024
- Immunoprecipitation assay - This lecture explains about the immunoprecipitation assay protocol. it explains the process of using antibody antigen interaction to separate proteins from mixture of proteins.
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Great lecture! I liked how you quickly gave an overall explanation of immunoprecipitation, and quickly moved on to more complicated related techniques like co-immunoprecipitation and pulling down. Very helpful!
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Thank you so much for this video! Immunoprecipitation makes much more sense to me now!
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ı have a question
how can we test if the A B C complex is formed or not?
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really very informative ........great
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Hi Shomu, I watched several of your videos, the content was great, but I think it would be better if you can produce the video in daylight when your shadows less affect your drawing...
What would be the effect of presence of activate RNA polymerase (transcript associated with it) in this process?
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generaly can u give me the princip of these method !
and is the number of antigen similar to antibody or no ! ............in ALGERIA we study in french this is why i ask u all the time hahaha .. u re great ***** thank u ...
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can we use western blotting after co-immunoprecepitation to detect a specific protein?
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+Apu Chowdhury yes. And we have to do that most of the times
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thank you
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very much helpful
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Explanation of the
"pull down" mechanism is not fully clear in this video..
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easy way of learning
+chhavi saxena thank you.
Great video thank you :)
17:00 So, in the end, bait is interacting with A because our bait is designed to only interact with A and not Y? in this case, then we already know our answer to have designed bait that only interacts with A and not Y. How does this answer the question of which molecules bait do actually interact with if we design a bait that has affinity to A? or is it that bait is basically unknown and we just tag it with fusion protein to see which protein it binds to in the end?
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Aynaz
Nice video
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Thanks sir
thanks
you did not explain that why Y did not interact with beadX-A complex?
Hey!
Can anyone explain, In the Pull down method; A will attach with X, but what stops A from interacting with Y, since they are known interactors :/
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shomu you are explain to a serotyp?
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lat part was little bit confusing
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