Elisa Test | Direct Indirect & Sandwich 🩸
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- Опубліковано 11 лип 2024
- #drnajeeb #medicaleducation #drnajeeblectures #spleen
Elisa Test | Direct Indirect & Sandwich 🩸
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▬▬▬▬▬▬▬▬▬▬ Contents of this video ▬▬▬▬▬▬▬▬▬▬
00:00:00 What Is ELISA?
00:00:40 Explanation Of Mechanism Through An Example
00:09:57 Addition Of Substrate
00:11:55 Advantages Of Direct ELISA Test
00:13:58 Issues And Modifications Of ELISA
00:19:50 What Is Epitope?
00:22:01 Indirect Elisa Test And Its Mechanism
00:26:50 Performance Of Direct And Indirect Elisa Test
00:33:48 Effect Of Adding More Enzymes
00:34:40 When To Use Direct Or Indirect Test
00:39:44 Purpose Of Using These Two Tests Together
00:42:32 Sandwich Modification
00:49:37 Difference Between Direct And Direct Sandwich Elisa
00:51:03 Indirect Sandwich Test
00:54:27 Rapid Summary
00:58:07 Question
01:01:25 Radioimmuno Assay And Its Comparison With ELISA
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The ELISA test is simple and straightforward. You’ll probably need to sign a consent form, and your doctor should explain the reason for doing the test.
The ELISA test involves taking a sample of your blood. First, a healthcare provider will cleanse your arm with an antiseptic. Then, a tourniquet, or band, will be applied around your arm to create pressure and cause your veins to swell with blood. Next, a needle will be placed in one of your veins to draw a small sample of blood. When enough blood has been collected, the needle will be removed and a small bandage will be placed on your arm where the needle was. You’ll be asked to maintain pressure at the site where the needle was inserted for a few minutes to reduce blood flow.
This procedure should be relatively painless, but your arm may throb a little after it’s done.
The blood sample will be sent to a laboratory for analysis. In the lab, a technician will add the sample to a petri dish containing the specific antigen related to the condition for which you are being tested. If your blood contains antibodies to the antigen, the two will bind together. The technician will check this by adding an enzyme to the petri dish and observing how your blood and the antigen react.
You may have the condition if the contents of the dish change color. How much change the enzyme causes allows the technician to determine the presence and amount of antibody
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Exceptional video...ELISA was never so easy until Dr. Najeeb came
well said
Best teacher in the world
I wish i had a teacher like u .. hardly i came across such a teacher who teaches with so much simplicity like u ...Great work sir..trillions of thanks will also be microscopic 😁😁 for u... still infinite ,infinite thanks to you .
No video on youtube can beat how Najeeb sir has explained this concept thank you sir
Wonderful teacher....A techer should be like u...
My mind exploded .... he is Sooooo amazing. Wished i had you as a teacher in school.
The topics which u don't understand anywhere in the world.. This is the solution.....u r a blessing for medical students sir...after watching this I can teach ELISA😁
when i listen your lectures i ask myself why other teachers do not clarify things like u ....then i remember that DR.najeeb is only one
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Dr.Najeeb... A man who with his midas touch can break medical mountains into minute marbles...
I was repeating along with your students! Thank you so much for this explanation. I look forward to watching more of your videos.
I didn't know that it was as easy as this, our lecturers were not doing well really. So simple, so easy , so fantastic and it has gone to the long-term memory without reading any book. The ever best medical lecturer in the world.
Dr. you're not only a blessing for MBBS students but for other health professionals as well. I am in the 4th semester of my pharmacy school and literally your lectures help build up my concepts a lot. I can't imagine passing with good marks without listening to your lectures. The lectures are fun and I never get fed up with long lectures of yours. JazakAllah, thanks a lot. May Allah bless you, Ameen !!! :) You're an inspiration, keep up the gooD work :D
agree❤️🌷
No words for this such a wonderfully explained lecture
Wow!! best teacher ever! i will never forget Elisa
You made me understand ELISA in just an hour. Keep the passion burning! You are a very good teacher.
Sir I don't have such a lecture in my life ..salute to your smart and great work....
Sir! You're the best teacher in the world.
I have my exam tomorrow and this video will help me score really Good.
Thank you
It was never as crystal clear before , Thank you!
Outstanding! Thank you so much!
He is a gift to people. Thank you so much !
Excellent! Dr. Najeeb!!! Thank you!
You are the worlds best teacher in medical field ,really indebted to you ..no one can teach like you ..believe me ..god bless you sir
sir u r great....u make things very simple...a kind of teacher like u is a blessings to our nations. may GOD gives u strength n bless u more n more.
This is insanely good. You make sure that the audience understand this. Seriously I cant thank you enough. I really cant believe it that I undestand it now easily. Thank you so much Dr. Najeeb
Thank you for your love and support. Stay blessed. :)
Thank you very much , it was awesome , i learnt and never forget this topic .
Thank you sir. Lifesaver and brilliant as always.
wow sir, I never watched any vedio like ur vedio
wow . very much encouraging me. Thank you sir. I pray for your health
I always find topics of immunology difficult to understand... but now it’s crystal clear.... Thank you so much sir.... such a passionate teaching...👏🏻
Excellent way of imparting the knowledge and skills required
Awesome lecture on Elisa... thank you
great. please have more lectures on assays, because the way you explain is very good.
pcr. rt pcr. northern blot. suthern blot. western blot. ihc.
thanks again
Really
Dr Bhanu Prakash has already made watch that
yes Sir please give us lectures on this topic
Another great lecture by a legend
REAL Dr , my lecturers makes this impossible to understand, thanks for amazing way of explaining Dr.
I was having many confusions regarding this topic sir but you made each and every concept crystal clear. Thank you so much. Now I'll never forget ELISA.😇
Happy to know that from you! ❤️
No words for teaching Skills......... Teaching and knowledge K duniya k betaaj BADSHAH ho Sir aap
Thank you so much sir for this lecture. You are the best i have ever seen. I want to teach like you. You make difficult things easy. Any topic i want to read, i must first search for it in your videos. ELISA has been a difficult one for me to understand but see how you clarified it with so much ease. I don't need to go back to my books to read ELISA. I have gotten what i needed.
Magnificent way of teaching which cleared the doubt that I have had for 2 years......... thanks sir. I extremely loved it
Thank you for your love and support.
i wish to see you dr najeeb one day and give thanks for your teaching i always see your lectures since 6 or 7 years it is magic i say your are my teacher ,i m from algeria thanks to you i succed in most of my exams ,god bless dr najeed .
Thank you for these amazing lectures that help all the medical students . I'm a medical lab sciences student and never understood this topic before . You're the best teacher in the world
His videos are for MBBS students ????.... For which year ??
Sir, what an explanation! Just now I have cleared all my concepts regarding ELISA. You made it very easy and fun to study.
Thank you sir
Thank you. This was very helpful.
This is most amazing explantation.
Thank you❤
So amazingly explained
This is called crystal clear concept...thank u so so much for lectures sir...we admire you the most
This is called the addiction of teaching ..!. Thank you so much sir !
Thank u sir.
This video give me a Crystal clear concept of ELISA which i will never forget in my life.It helps me a lot.
wowwww salute to this great personality, best teacher who are educate the humanity with the absolute authentic knowledge. billionsssssssssssssssssssssssssssssss of thanksssssssssssssssss
Sir teacher ho to aapke jaisa.... Everytime I watch ur videos u make me fall in love with that topic... Huge respect for u sirji... A real GURU❤lots and lots of respect to u sirji🙂
Awesome lecture, everyone will love to see all your lectures
Best concepts about elisa i ever got
thank you so much Dr. Najeeb for this amazing ELISA lecture which really helped me a lot 💚💚💚
Phenomenal explanation! Thank you
thank you dr.najeeb simple and understandable
absolute legend 🙏🙏
Salute to you sir. Believe me I haven't watched a movie with that amount of concentration as I do watch his lectures.
Thank you sir.
Thank you sir for love. ❣❣
Thank you with love
Absolutely amazing. Thank you sir 🙏👍
I never thought this topic can this much interesting
Best explanation till date 😊
You are a great teacher thanks for giving best knowledge ❤️❤️
thanks never enough for u ~
ELISA is that kind of topic which am unable to get in organized, clearified and easy way,
M thankful to you #NAJEEBSIR
ALLAH BLESS YOU
Have subscribed to your channel and listen to your videos over and over again.
A teacher should really be like you.
Please keep making videos. Can you please make a video on chemiluminescence. It's mechanism, how it emits light and how we measure it.
Thank you so much for your love and support. Suggestions by my beloved students are highly appreciated. New lectures are being recorded and will be uploaded soon.
Fantastic class and Very clear everything.
if messi is gifted with unparalleled footballing skills, dr najeeb is blessed with unparalled teaching skills in the field of medicine. what amazes me is his ability to explain diverse & unrelated topics with such clarity, which even domain specific teachers couldn't explain in our colleges. at times i wonder how much better, we would have been as doctors only if we had teachers like him in college.
Thank you very much for your explain sir, this can be understanding to me about the beautiful knowledge.
You are most welcome❤ Keep supporting❤
Thanks for wonderful lecture
I am a phd scholar ,But over the years I have read elisa was not clear to me,but the concept and the way you explained the topic, made me crystal clear about ELISA,well explained sir... Thanks a lot....Regards Mayank Roshan
Glad you found this lecture easy to understand and meaningful! Stay blessed💗
great way of teaching thank you very much sir
Best ever seen explaination of elisa.....quite awesome...
your video is very helpful ! thank you so much ! love from Israel ♥
Sir you are a genius lecturer sir, I don't understand ELISA but after seeing the lecture of you I came understand.
Thank you so much Sir to give the ELISA lecture.
Brilliant thank you
You're very welcome❤
Love you alot sir ❤️
Your way of explaintion is simple and understandable
Thanks alot for your efforts
You make such a easy 😮😮
U r genius sir... I'm a bscl mlt lab technician...... After 5 years I got it
I am a retired Professor of Zoology. Your way of explanation is impressive. Congratulations Sir.
Thank you very much teacher
Very very helpful...thanku sir😀
tnx it was very helpful 🙏🏻
Finally understood the topic ELISA
U make it very clear sir thanku so much
Great lecture Sir. Thankyou.
Thank you Sir🙏🏼
Thank you so much sir..... Prior to see this video I am so confused about elisa now I am so cleared....
Just one word , excellent sir
Incredible way of explanation 🤗. I wish that u explain every subject in this way 😍
Wooooooooow
Wonderful lecture sir, Way of explanation is very good. Learning with Indian English really touches the students heart and Mind....
HATS off for you Dr.najeeb i like your way of teaching and in near future i would have hair style of your's.
thanks for this lacture
Sir u r a life saver..... May Allah ji bless u more and more
Ur teaching is so clear that i can understand it so easily even though i am bad at english.
I never understood ELISA before, it was just mugging off for exams only. Thanks a lot sir.
Pratibha Padhi same here
I was struggling to understand this but righ now I completely get it! Thank you
Same hre
Thank you💚💚💚💚
You are my great Teacher. ! Hope one day we will meet.!
You're good than my fav lecturer.
Thank you so much
I wished I'll get all med school lecture from you
You are welcome❤ Keep supporting ❤
U r the greatest Dr ever
LOVE YOUR LECTURES. LOT OF RESPECT.
Thank you so much :)
Thank you sir
Your teaching way is very good...
Assalam o alaikum
Sir , you are the best teacher👍💟 I have seen who cleared my concept about this topic I have seen many many lectures but today I have clearly understood what it is ??
Thank youuuuuuuu soooooo much sir .May Allah bless you Aameen
Excellent explanation sir👍👍👍👍👍