Chromatin Immunoprecipitation (chip assay)

[@atefalmalky9268]

FACT, you have been my professor for the whole semester. thank you

[@shomusbiologyofficial]

+ATEF Al thank you.

[@sartsoul]

Most people just do common topics bcoz that fetches more views. But you have covered such particular topics too. Hats off!!

[@shomusbiologyofficial]

Thank you

[@rajeshj.6919]

Teaching god for 2k bioscience people.......Thanks a lot sir....you made bioscience easy !!!

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

[@LALFAST]

Forget about ChIP; did anyone else get amazed by the near perfect circle at 2:48 ?

[@elliottdaxton7930]

I know im asking the wrong place but does someone know a way to get back into an Instagram account..?
I somehow lost the login password. I would appreciate any assistance you can offer me!

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@Elliott Daxton instablaster :)

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each video i watch from your channel makes me so confused bc HOW can you explain things that have been so hard for me to get so easily??? no, really, you're truly gifted

[@shomusbiologyofficial]

Thank you

[@nesrinehaif1326]

To be honest with you im preparing for my phD but if you were my teacher i would be the best student i only found you now BRAVO you made me love my major more and more

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@nesrinehaif1326]

@@shomusbiologyofficial Thank you for you for being the best teacher

[@Mettephobicemily]

You have helped me with your videos so much, thank you for doing this.

[@shomusbiologyofficial]

Thank you.

[@srijanikarmakar7970]

Darun sir ❤ amar ei topic tay ektu somossa chilo bole youTube e ese search ditei 1st ei apnar video ta dekhlam... Sotti e sob doubt clear hoye gelo 😊.. It was totally helpful ❤

[@shomusbiologyofficial]

You're welcome. Stay tuned for more

[@ayeshasaman6401]

This guy is my teacher ♥️♥️♥️❤️❤️❤️❤️ you have helped me in every exams of mine

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@TmacDominguez]

Thank you so much for your breakdown video!! I am in a genetics class and having difficulty understanding these methods. Thanks!

[@mariettadonatella4426]

thank you so much for this video! I've been having difficulty properly visualizing and understanding this concept and you've helped me finally comprehend it! Really, thank you so much!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@nachinathan3846]

Explained simply.. Thank you sir

[@maximilianovallejos3301]

Applications Scientist here. Excellent explanation! Keep it up!

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures

[@Arendt-Foucault]

nice, simple and straight forward handy stuff.

[@陳瑋圻]

huge help for my presentation

[@shomusbiologyofficial]

Thank you

[@paulbevins1087]

Such an amazing explanation. Thank you!

[@adnanshah7634]

Thanks! I understand it now!! Why couldn't my professor explain it like this?!

[@bnijj]

thanks, my professor doesn't have use a textbook so this really helps!

[@kaavyasurianarayanan8247]

Greatest teacher ever!! Happy Teachers' day Sir!!

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures. Please subscribe and share

[@revathyb5526]

awesome online professor!!!!!!!

[@shomusbiologyofficial]

+Revathy Sarah thank you. Glad you liked my lectures

[@Dm-hz9mm]

Shomu, you're a boss. Thank you

[@shomusbiologyofficial]

You're welcome

[@cynthiagomez2081]

You are saving my semester. THANK YOU

[@shomusbiologyofficial]

+Cynthia Gómez glad to hear that

[@UsmanKhan-fp5uu]

Brilliant presentation

[@shomusbiologyofficial]

Thank you

[@mayukhroy5393]

thank you, Sir. wonderful explanation.

[@shomusbiologyofficial]

You're welcome

[@alinashrestha7324]

you are really awesome. i mean you start off so simply. its magic to watch your videos. helped a tonnnnnnnnnnnnnnnn!!!!!!!

[@shomusbiologyofficial]

+alina shrestha glad to hear that. Please subscribe and share

[@wafaleulmi6321]

thanks again , it was very helpful ☺️

[@shomusbiologyofficial]

You're welcome

[@educationinfo5080]

Our great professor ❤

[@shomusbiologyofficial]

Thank you

[@diamondmitchell9567]

You explain everything so well! It all makes sense! Thnks

[@shomusbiologyofficial]

You're welcome

[@psychtogo4291]

Thanks a lot for all the videos you are uploading here! It saved my life :D You are very good at explaining things easily, great Job! Even I could understand and follow you. :)

[@shomusbiologyofficial]

+Lea Davinci thank you so much for supporting

[@bengisumolyer7310]

Hello! This video was very helpful, thank you very much :)

[@shomusbiologyofficial]

+Bengisu Molyer glad it helped

[@samoleulmi990]

another great video, thanks for your efforts sir 🙏

[@shomusbiologyofficial]

You're welcome

[@mr.abundant6449]

Thank you so much sir. Your involvement while teaching is good and covering all the useful topics of life sciences. Great deed......

[@ismaildin69]

Your Hindi lectures are more interesting and easier to understand kindly sir explain in Hindi.

[@shomusbiologyofficial]

It's because you don't understand English well

[@Juliaardbei]

Thank you! It was very clear!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@soadbohorquez1384]

Thank you so much! you are a life saver!!

[@geovannig8849]

thanks for the video .Very helpful in my studies.
Note: aldehyde not aldehide.

[@ratty1942]

Shomu, thank you sooo so much for all you work here on UA-cam. You've helped me countless times, really. Keep up the great work, you're one of the best out here :)

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@LeafwortThumbfloggin]

Shomu to the rescue!

[@SLotusSutra]

Thank you so much! It helps me a lot

[@aiwysaur6272]

Thank you so so much! You are my savior in this era of COVID-19 quarantine

[@rayanhamadeh7321]

Very helpful thank you

[@shomusbiologyofficial]

You're welcome

[@eladophir8959]

Great video, many thanks brother,

[@juanport7106]

Does the cross linking caused by formaldehyde affect its sequencing at the end of the process?

[@revathyb5526]

revere crossing linkings are done at end and the dna part is sequenced .....using PCR........ bla ...bla

[@pritipragyanswain4470]

thnk u sooo much sir. ur video are really helpful especially b4 xm.

[@darulizakernain7758]

u r great... i love watching ur videos... keep it up

[@sanagull7611]

very good explanation.

[@samikshyabhoi8448]

Thnku so much.... Please upload more video

[@MeeJaidee]

Great explanation and love your accent :)

[@finalflash929]

this was damn helpful, clear as hell

[@shomusbiologyofficial]

+finalflash929 thank you

[@cathytang2558]

Very nice video! One question I have is why the addition of formaldehyde would help the protein binds more tightly to the DNA?

[@Knautschewolke]

thank you very much for this video, it helped a lot!!
well done :)

[@nathanxu666]

Hi, I was wondering what the proteins of interest usually are for ChIP, as I'm a bit confused of the overall significance of this assay relative to chromatin. Thanks so much and I loved this video!

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@besmabrewer6938]

Thank you for explaining this so well!!! :)

[@shomusbiologyofficial]

Glad you liked it

[@MrM7modgrayeb]

thank you sir, very helpful

[@tiffanydoan2481]

so greatful for your videos! thank you so much for doing this

[@shomusbiologyofficial]

+Tiffany Doan glad you liked it.

[@agatamystkowska5803]

Shoum, youre the best

[@sarahabaza9684]

Dude you're awesome ❤️

[@layla2387]

super helpful, thanks!

[@shomusbiologyofficial]

You're welcome

[@NumairMansur]

Thankyou bro ! helped me a lot

[@aryaram8192]

Thank you Sir😊

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@adricortee7289]

Thank you for all your help!

[@AlecPlaisier]

thanks, good vid! wasn't so hard after all

[@ahmadmuqbil3523]

Thank u sir 💚💚

[@shomusbiologyofficial]

You're welcome

[@unFayemous]

thank you very much, this was very helpful indeed!

[@adrianadominguez2285]

Thanks so much!!, i have a question, what its the difference between ChIP on ChIP and ChIP-seq?

[@samgraves8703]

thank you shomu

[@shomusbiologyofficial]

You're welcome

[@mshisha]

Thank you!! You're a hero.

[@shomusbiologyofficial]

+Lynn Mina thank you very much for the appreciation

[@budhadityagayen4214]

My boy Shomu about to drop the scariest ghost story of the Century!!

[@shomusbiologyofficial]

Ha ha

[@gafapatty1611]

your videos are really helpful :)
is chip sequencing and chip assay same?
please tell me i have exam tom.

[@baek_hyunee3418]

Thankz toh bnta h 🤗🤗🤗

[@remygomet7690]

Thanks the video, now I get it !

[@Hoenest]

Thank you!

[@shomusbiologyofficial]

You're welcome

[@caualima9533]

helped me a lot, thanks!!!!

[@divineconnectiontarot]

very helpful

[@mariomyousef6994]

Please, How can I make bacterial count for tuberculosis

[@Diary_of_Yuki_the_cat]

Thank you sooooo much for this video!!! I finally could understand it. :)

[@vaishalithukral9129]

When we are adding formaldehyde, will it cross link other protein-dna and protein-protein in the cell too other than our target protein-DNA ? Thankyou

[@shreyadas3278]

Thank you

[@shomusbiologyofficial]

You're welcome

[@loveleensingh6365]

Thank you bhai

[@shomusbiologyofficial]

You're welcome

[@nishadhenwal7756]

Thank u🙏

[@shomusbiologyofficial]

You're welcome

[@abdoulmadjidkone1833]

You're the best!

[@mahyarsh87]

thank you. was helpful

[@lokkhichana]

Very helpful.

[@adeniyisalami9416]

you know your stuff bro...

[@rishikeshkade7827]

Always best

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@Racekelly9]

Hi! I really liked your video, very didactic, thank you!!!
But I have a question, how do we know that the endonuclease enzyme will cut exactly where the protein is binded?
I mean, if it cuts a little more of the sequence that the protein is binded, when you isolate the sequence with the antibodies, the isolated will no be only the region which the protein binded.
So, how do we know where the enzyme will cut?

[@Ansarali-uo5ng]

The enzyme he mentioned i think is wrong I am not sure i think the is MNase

[@mariafu693]

Hello Sir, can you please tell me the difference between this EMSA and CHIP-qPCR? Thanks.

[@concept_of_BiologyAS]

thank u sir ji

[@shomusbiologyofficial]

You're welcome

[@ΒασιλικηΠανταζη-φ1δ]

many many thanks!

[@shomusbiologyofficial]

You're welcome

[@karimiqbalsworld2809]

Hi, i want to know the minimum concentration of Template of ChIP DNA usually used for qPCR?

[@afafsaliba7299]

Excellent

[@shomusbiologyofficial]

Thank you

[@isabelleus2881]

really helpful!

[@akshayavidhya9325]

THANK YOU SO MUCH SIR.

[@priyadeepghosh9610]

I didn't understand the last portion. After precipitation we found the protein attached with bead so what is the need of loading the protein in gel??

[@juancarlosrohrer8624]

thank you very much

[@shomusbiologyofficial]

+Juan Carlos Rohrer you're welcome

[@cartoonfactory8707]

u r boss brother

[@shomusbiologyofficial]

Thank you

[@jackieal100]

good video thanks

[@sleshapanthee4709]

oh god tu si great ho

[@shomusbiologyofficial]

Thank you

[@jorgecabrera-moreno7649]

Dude! thanks a lot

[@shomusbiologyofficial]

+Jorge Cabrera you're welcome

[@junaidkhan-lx2hv]

Thank u so much