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Starting RNA work for the first time so this was very helpful, thank you!
Just one thing I hear that it is good technique to keep the pipet vertical at all times.
This is a great resource, thanks!
Can I lyse the cells directly from the vessel? And after this protocol, is it necessary to do a RNA cleanup or I can skip that?
I am extracting RNA for RNA seq from mice skeletal muscle. How much in weight should I use to make my lysate with what volume of RTL?
She used high speed both times lmao
Thank you :)
Starting RNA work for the first time so this was very helpful, thank you!
Just one thing I hear that it is good technique to keep the pipet vertical at all times.
This is a great resource, thanks!
Can I lyse the cells directly from the vessel? And after this protocol, is it necessary to do a RNA cleanup or I can skip that?
I am extracting RNA for RNA seq from mice skeletal muscle. How much in weight should I use to make my lysate with what volume of RTL?
She used high speed both times lmao
Thank you :)