This is a guided video for RNA isolation in animal cells. If you need an explanation in Korean, you can watch it at the address below. • RNA isolation by TRIzol
Very helpful video <3 Btw, I want to ask about your last step that you heated the RNA tube with the lid opened. Normaly in our lab, we just try to take out the Ethanol as much as possible and let it dry naturally but it takes kinda long time. I just wonder if heating the RNA without water could affect RNA's quality or not and is that safe to open the lid outside of the safety cabinet (even with the nilon cover as you described). Thank you so much!!
Hi, it's a really helpful video. I am wondering about the step of after adding the chloroform. Would slightly heavier shake would be helpful to form a easier phase to extract the RNA layer?
The reason of incomplete phase seperation after chloroform is less mixing..a good shaking of tube will mix all ingredients and forms clear 3layers
this is so so helpful. Thanks a lot
Woohoo! an English version of this :D
I am surprise you heated the RNA, I haven't seen or heard of such method before. isn't it going to degrade the RNA or something like that?
Very helpful video <3 Btw, I want to ask about your last step that you heated the RNA tube with the lid opened. Normaly in our lab, we just try to take out the Ethanol as much as possible and let it dry naturally but it takes kinda long time. I just wonder if heating the RNA without water could affect RNA's quality or not and is that safe to open the lid outside of the safety cabinet (even with the nilon cover as you described). Thank you so much!!
Hi, it's a really helpful video. I am wondering about the step of after adding the chloroform. Would slightly heavier shake would be helpful to form a easier phase to extract the RNA layer?
Hi, very helpful video thanks ! why do you use 75% ethanol and not 100% ethanol ?
I wish we could see how the extract looked like in an agarose gel. :D
Hi it's a really helpful. Thank you.
Hello, what’s the purpose of rnase water ?