How to purify microbial and host DNA from stool samples

Learn how to isolate microbial DNA that accurately reflects the diverse microbes in the community sampled. This video will provide an outline of stool microbial DNA isolation, plus some tips and tricks. In addition to stool, the PureLink™ Microbiome DNA Purification Kit can be used to isolate DNA from urine, saliva, swabs, transport media, microbial culture, and soil.
The online manual describes the protocol in detail: tools.thermofisher.com/conten...
For more information on PureLink™ Microbiome DNA Purification Kit, visit: thermofisher.com/microbiome

[@laurengregory5843]

I’m looking to be a part of an undergraduate research program to, in lamen’s terms, collect and analyze otter poo. I have never done anything of the sort so I wanted to research how poo was analyzed.... I’m shocked and amazed by how much work goes into it

[@thermofisher]

Science takes us to many obscure places...many of which require washing your hands.

[@MsRabiabatul]

Can I use this kit to isolate protozoan sppecies of eimeria

[@shanefitzgerald9339]

Great video thank you! Is the protocol the exact same for swab samples?

[@thermofisher]

Hi Shane. Thank you for your feedback and question.
The protocol for swab samples is available on the product page. www.thermofisher.com/order/catalog/product/A29790?SID=srch-hj-A29790#/A29790?SID=srch-hj-A29790.
For additional questions, please contact our technical support team at thermofisher.com/askaquestion.
Thank you!

[@MrFilu13]

Hi plz clearify
the equality of extracted stool DNA is good enough to go for metagenoomic study??
That 260/280 raito we'll get?

[@sherines1918]

Is the protocol the same for oral samples ?

[@lilianjose5907]

Is the method different for the extraction of microbial and host DNA? Is there really an obvious result between the two DNA?

[@lilianjose5907]

@@thermofisher Thank you!

[@thermofisher]

Thank you for your question.
There isn’t a way to distinguish between microbial and host DNA but if you do have questions about one of our DNA extraction kits, please contact our technical support team at thermofisher.com/askaquestion for additional information. Thank you!