When I worked in the Biotech field years ago, I would use a stir plate, pour agar into flasks until fully mixed, then autoclave and when temp is cooled, I would pour into petri dish under a hood.
Every channel has a different recipe for an Agar dish. For those of us who haven't worked in Biotech the question is Why? Do different bacteria, etc need to be grown in their own recipes?
Disclaimer: I'm not an expert. Most bacteria can grow on LB agar (the one used here). If you are trying to do selection (wanting only a certain type of bacteria to grow), you would need different media or adding antibiotics.
@@ahmadfadda2654 Nah, thats what pressure cooker sterilization and the hood is for. The whole point being to have a sterile agar dish which then you introduce only the bacteria you want grown into.
@@charleswetzel1250 Any internal reference. But my point was to NOT label the lid as that separates. Label the actual bottom where your bugs are going.
Needs better instructions like what is t type of agar you used where you got it how much water and agar intervals of heat, temp the agar needs to be before pouring list of supplies
I tried growing bacteria on petri dish but havent got any result plates still looks the same after 7days & also my agar solution is whitish not yellowish as yours
@@DevoutJourney if you don’t use a pc and get contamination is because there are bacteria everywhere and the pc is used to kill them if you don’t kill them your media will become contaminated
When the media is done sterilising wait for it to cool down till you can start to see it get thicker and then pour if you pour around 50c there won’t be any condensation
I wouldn't personally this is a hack method and if it works it can show the shortcuts and how to bypass some time and teks but also there is a reason succesful people don't perform this way
When I worked in the Biotech field years ago, I would use a stir plate, pour agar into flasks until fully mixed, then autoclave and when temp is cooled, I would pour into petri dish under a hood.
Every channel has a different recipe for an Agar dish. For those of us who haven't worked in Biotech the question is Why? Do different bacteria, etc need to be grown in their own recipes?
Disclaimer: I'm not an expert.
Most bacteria can grow on LB agar (the one used here). If you are trying to do selection (wanting only a certain type of bacteria to grow), you would need different media or adding antibiotics.
@@ahmadfadda2654 Nah, thats what pressure cooker sterilization and the hood is for.
The whole point being to have a sterile agar dish which then you introduce only the bacteria you want grown into.
Pro prop tip: Don't label lids. Label the base.
What exactly are you labeling on them? The date it was poured and what media type it is?
@@charleswetzel1250 when you only growing one strain you can just do the date they were made
@@charleswetzel1250 Any internal reference. But my point was to NOT label the lid as that separates. Label the actual bottom where your bugs are going.
Needs better instructions like what is t type of agar you used where you got it how much water and agar intervals of heat, temp the agar needs to be before pouring list of supplies
Yeah that’s correct….
I tried growing bacteria on petri dish but havent got any result plates still looks the same after 7days
& also my agar solution is whitish not yellowish as yours
What do you use as the media is it just pure agar agar or does it have some additives?
You can make agar with a lot of stuff I make mine with light malt extract
@@ballpythonlover10 intresting I’ve never heard of anyone much other than broth. Would you mind sharing your recipe for agar with malt extract?
@@physics3632 500ml water
10g of agar
7-10g light malt extract
Using this method, without PC the liquid, how’s the contamination?
@@DevoutJourney if you don’t use a pc and get contamination is because there are bacteria everywhere and the pc is used to kill them if you don’t kill them your media will become contaminated
How did you prevent condensation
Once you pour the agar and as soon as it solidifies immediately turn the dishes upside down.
When the media is done sterilising wait for it to cool down till you can start to see it get thicker and then pour if you pour around 50c there won’t be any condensation
@@kreature7946 condensation not contamination
@@randyorton1769 yes, the plates are stored upside down to prevent condensation not contamination.
I dont understand a thing when seeing this video. More explanations please
Senjougahara best girl
Everytime I make my agar it comes out wrong and I don’t understand what I’m doing wrong.
Pressure cooker
Do not follow these instructions kids (unless you want contam everywhere).
Microwave 😳 dose that really work ? Equivalent to 15 psi ? How long to microwave for ????
I wouldn't personally this is a hack method and if it works it can show the shortcuts and how to bypass some time and teks but also there is a reason succesful people don't perform this way
@@rubenbarela I think your correct 😉
I don't know, but seems the high temperature of the microwave is just fine
What’s the fkn recipe wtf
1st
I like you nick name just I think other way around is also correct.
RIP💩E Loos 🪰🎪