A really good and a clear lecture. Honestly... u have no parallel... I do mean each word!. I understood everything in one go!. Now, I have some doubts, 1) Do we always have to use a rectangular plate? 2) Why doesn't the retention factor depend upon the thickness of the plate? 3) Can we calculate the kinematic variables of the rate of rise? 4) What are the factors that affect both the products found in the mixture and the solvent that affect the acceleration and retardation of the respective compounds? 5) In the solvent and solute in the example given, is there an initial acceleration? if yes, upto which position does it accelerate? and then, how will the deceleration depend upon the acceleration?
This dude is so knowledgeable; he has helped me a lot in ochem/biochem, but I kind of can't take the way he is screaming at me... it's almost too much lmao
You keep mentioning that the stationary phase is the plastic plate. It’s not. It’s simply a means of supporting the stationary phase which is a highly polar substance. A very common stationary phase in TLC is silica gel. So, in such a case, it’s the silica gel which is the stationary phase and the components of any mixture added to that phase will be adsorbed, or bound to the silica gel to varying degrees, depending on the polarity of the individual components. Less polar components will travel up the plate at a greater rate than more polar components which will be more strongly attracted to the surface of the stationary phase. Another common support for the stationary phase is glass.
Yep, and it could also be alumni for TLC which is slightly less polar than silica. Anyway what confuses me the most about TLC is that the stationary phase is highly polar, and for our substances to travel up the plate they need to be soluble by the mobile phase, which could be polar or non-polar, so if our mobile phase is non-polar and the substance is polar, then it will not be dissolved by the mobile phase and it will have high attraction to the stationary phase so it really won't move much or at all, however if our substance is non-polar, and our mobile phase is polar meaning it still won't dissolve the substance, then that means the substance doesn't have attraction to either of the phases, so what happens? Does our retention factor stay at or close to 0 as well in this case?
We are addressing this topic in Organic Chemistry Lab now. I do not remember this being taught in Bio I or II. It would be interesting to see what unit in Bio you were taught.
@@SolaScriptura49 we are studying this in college in biology chapter 1of first year and chapter name is cell structure and function. This is in the topic of techniques for studying cell structure
Very interesting, I do not remember that being a topic in bio I, what a great introduction though!! We just finished recrystallization and are now in solubility in orgo. I would say this is used both in bio and chemistry.
your explanation is very simple and clear. Thanks a lot brother.
Thanks so much sir ,I haven't understood this from my teacher but I got it clearly today thanks so much I will keep on following you
your explanation is very easy to understand.. thank you so much sir :))))))
A really good and a clear lecture. Honestly... u have no parallel... I do mean each word!. I understood everything in one go!. Now, I have some doubts,
1) Do we always have to use a rectangular plate?
2) Why doesn't the retention factor depend upon the thickness of the plate?
3) Can we calculate the kinematic variables of the rate of rise?
4) What are the factors that affect both the products found in the mixture and the solvent that affect the acceleration and retardation of the respective compounds?
5) In the solvent and solute in the example given, is there an initial acceleration? if yes, upto which position does it accelerate? and then, how will the deceleration depend upon the acceleration?
I love u so much, honestly u are a genius. ur explanations are just perfect.
Does the mobile phase contains a solvent and an analyte
Does the stationary phase contains a column and solvent?
thank you, you are a great lecturer
Could you please do one on HPLC?
Thanquhh so much you should teach at my college in place of my professor
how can solid material will be stationary phase
So where does paper chromatography fall
Very clear explanation. Thank you
Is Rf1 and Rf2 are value from the same compound?
Good
This guy is good.
You presentation materials are very informative but your lectures are so fast that its really hard to understand!
Many thanks ❤❤❤❤❤
Thanks ❤️❤️
This dude is so knowledgeable; he has helped me a lot in ochem/biochem, but I kind of can't take the way he is screaming at me... it's almost too much lmao
Much respect!!
AMAZING EXPLANATION SIR
Superhumans do exist like him
thanks
You keep mentioning that the stationary phase is the plastic plate. It’s not. It’s simply a means of supporting the stationary phase which is a highly polar substance. A very common stationary phase in TLC is silica gel. So, in such a case, it’s the silica gel which is the stationary phase and the components of any mixture added to that phase will be adsorbed, or bound to the silica gel to varying degrees, depending on the polarity of the individual components. Less polar components will travel up the plate at a greater rate than more polar components which will be more strongly attracted to the surface of the stationary phase. Another common support for the stationary phase is glass.
Yep, and it could also be alumni for TLC which is slightly less polar than silica. Anyway what confuses me the most about TLC is that the stationary phase is highly polar, and for our substances to travel up the plate they need to be soluble by the mobile phase, which could be polar or non-polar, so if our mobile phase is non-polar and the substance is polar, then it will not be dissolved by the mobile phase and it will have high attraction to the stationary phase so it really won't move much or at all, however if our substance is non-polar, and our mobile phase is polar meaning it still won't dissolve the substance, then that means the substance doesn't have attraction to either of the phases, so what happens? Does our retention factor stay at or close to 0 as well in this case?
God level
This is in biology course not chemistry
Your view is too narrow. I've took IB Bio and AP Chem and both courses cover this topic.
@@lynny7868 ohh
We are addressing this topic in Organic Chemistry Lab now. I do not remember this being taught in Bio I or II. It would be interesting to see what unit in Bio you were taught.
@@SolaScriptura49 we are studying this in college in biology chapter 1of first year and chapter name is cell structure and function. This is in the topic of techniques for studying cell structure
Very interesting, I do not remember that being a topic in bio I, what a great introduction though!! We just finished recrystallization and are now in solubility in orgo. I would say this is used both in bio and chemistry.
how can solid material will be stationary phase
Hope so bro you are clear now with this point after two years 😂..
Hope so you are clear now with this question after 4 years😂😂