How To Use Plant Growth Regulators For Plant Tissue Culture
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- Опубліковано 2 жов 2024
- Have you been learning how to plant tissue culture and left wondering How To Use Plant Growth Regulators For Plant Tissue Culture? Our lab director, Francisco, has got you covered in this video! Use this link to save 10% off your first purchase: www.plantcellt...
This must be one of the most asked questions we receive. So this video covers what plant growth regulators are, how to use them, which ones to use, and how much to use.
🔗 Timestamps:
0:01 Intro
0:20 What are Plant Growth Regulators (PGRs) or Plant Hormones
3:22 Auxins (IBA, IAA, NAA, 2,4-D)
15:07 Gibberellic acid
17:04 Ethylene gas
20:15 What is the dose for houseplants?
23:21 What is the dose for carnivorous plants?
23:51 Plant Tissue Culture Master Class
10:07 Cytokinins (BA, Kinetin, Thidiazuron 'TDZ')
24:28 Outro
🛒 Get the Plant Growth Regulators You Need:
IBA Solution: bit.ly/3ZysQKA
NAA Solution: bit.ly/3LsMP9p
BAP Solution: www.plantcellt...
TDZ Solution: www.plantcellt...
Kinetin Solution: www.plantcellt...
Biocouplers™: bit.ly/3J3aD0r
Biocoupler™ Glass Set: bit.ly/3mHm6v3
Biocoupler™ Bundle: bit.ly/3Jui3eR
Laminar Flow Hood: www.plantcellt...
🎓 Exclusive Master Class Alert! After this tutorial, take your skills to the next level with our comprehensive Tissue Culture Master Class. Dive deeper into advanced techniques and hands-on sessions to become a tissue culture maestro! Register at plantcelltechnology.com/master-classes/
👍 If you found this tutorial helpful, give us a thumbs up, share with your fellow plant enthusiasts, and drop a comment with your experience and questions!
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I love how FAST you created a video from the time you posted the questionnaire on Instagram. Well done!
Thank you for your kind words, and thank you for keeping up with our updates ❤
Can make a video on somatic hybridization
Have you seen any recipes for majesty palms? Is it best to callus a piece of a leaf then multiply?
No, we didn't try the plant in our lab yet.
Can someone connect me ,I'm looking for a plant tissue culture job, I've two yr experience and three years diploma in Applied Biology
@plant cell
elche produkte von ihnen benötige ich für in vitro für cannabis... und welche eigenschaften bewirken die benötigten produkte für cannabis
Where do have your outlets in qatar?
We have distributors at all locations. You can find the details of the closest to you here: www.plantcelltechnology.com/distributors/
Hello friends.
I needed blue berry plant tissue culture, protocol.
Hi,
We haven't worked with blueberry plants in our lab yet, so we don't have a developed protocol for the same. However, you can try searching the internet, you will find many research papers mentioning the protocol that you can use.
Thank you for making this! Please do a video on the different types of bioreactors next!
Thanks for your love and support. Many great videos are lined up so stay tuned!
Which hormone suits plant having bulb as tubers, like hyacinth, tulips etc.is jasminic acid a hormone used for this type of plants
do you know where to find Piper auritum in a jar? Thanks for a great show!
If you are looking for in vitro plants. Try searching on Instagram. There are many into the business, so you might find what you are looking for.
Hey what's your stance on BA / BAP? The content creator from PlantsinJars had made a comment in some videos of her that BA is not considered safe because its synthetic. I know the MSDS sheet has safety precautions for it, but should hobbyists actively avoid it? Would touching media that has PGRs be harmful?
Here's what Francisco says: "That would be a personal opinion. It's like eating only organic products. Synthetic PGRs are preferred in tissue culture because they are heat resistant. Natural PGRs are usually not heat stable and must be added to the media after sterilization. This means you have to filter, sterilize, and add them to the media after cooling to 55°C. All these steps must be done inside the laminar flow hood."
@@PlantCellTechnology appreciate the response! You guys are the best
@@dragonsimon1 Thank you so much for your kind words!
Can you use/add the IBA and/or BA directly to soil media to help with roots or shoots development? If yes, how much?
Yes, you can use IBA (indole-3-butyric acid) and BA (benzyladenine) directly in soil media to promote root or shoot development. However, it's crucial to use them in appropriate concentrations and consider the specific plant species and growth stage.
@@PlantCellTechnology Very good. Suggestion on how to determine the appropriate amounts or where to find this info?
@@steve37341 You can either search online or try different amounts in different plants to see which ones working the best.
@@PlantCellTechnology Thank you sir! Will do both!
Amazing 😍
Thank you!
Very good video. Thanks for sharing.
Can you explain about the dilution of the hormone stock solution? The hormone is sold in powder form, measured in grams, but the protocol recommends using it in ml. What is the concentration of the stock solution?
Do you use any solvent to dilute the powdered hormone to make the stock solution?
Hi,
This video will show you how to make stock solutions of powder from plant hormones: ua-cam.com/video/XI3pH_X_4TI/v-deo.html
It will answer your quetsions.
Thanks for the video. You grow philodendrons, alocasias, monsters, carnivores...
And what about orchids - are you not planning them??
Currently, our focus is on houseplants and carnivorous plants. But, we will let you know if we plan orchids TC.
Thank you for all the informative videos! Would love to see a video on possible TC experiments one could do in a home 'lab', besides differences in protocols, and more in the direction of hybridization, creating somaclonal/genetic variability, etc.
Thanks!
Sure! We are trying to create more interesting and innovative content, so stay tuned to our channel!
Muchas gracis I live your content, I am a grower from homestead florida, I have used these hormones before and on active growing large plants and the response is very observable.
Thanks. Happy to know that you are watching our videos. If you require these plant hormones, you can purchase them from our store: www.plantcelltechnology.com/plant-growth-regulators/
Blue berry plant tissue culture media components.
Proportion.
We haven't worked with blueberry plants yet, so we can not provide more details. However, you can find the plant protocol by doing an internet search.
Thanks for your video. I have a question about GA3. Do you add GA3 into medium before or after autoclave?
GA3 is heat labile and should be filter sterilized and added to cooled MS media. So, add the hormone (using the filter sterilization technique) after the autoclaved media is cooled down.
@@PlantCellTechnology Thanks for your answer
Happy to help!
@@haohaongo
Great content. Please make more videos!
Thank you! Will do!