Restriction Enzymes in Molecular Cloning

[@AbCd-kq3ky]

I wish I had more time so I could watch all of your videos, but some of your videos have been really helpful in the past. I like how you don't shy away from details.

[@theCrux]

Thank you for your support and kind words! I am glad the content is useful :)

[@Messssyybun]

U r a blessing.. U should go through CSIR NET syllabus ( India) .. if u will make videos unit wise ..u will gain hell alot of followers ..god bless you ❤️

[@xenomeag]

Sir can you upload a video about dna polymerase types and their specific activities in both prokaryotes and eukaryotes 😊😊

[@snakepliskin6391]

I assume the restriction enzymes that only have one enzyme to perform both R and M function dont ever cut anything consistently because they modify the dna before they get a chance to cut it??

[@theCrux]

Yes, absolutely! So if you were to use it in molecular biology application, it would be very inefficient. People have tried to split the R and M out of the single enzyme but single enzyme loses activity if you force split. Type 1 are in addition notoriously terrible because they cut 1 kb away from their recognition sequence and cuts are never specific. As I write this comment, I wonder if it would be worth making a separate video about molecular and functional differences among the types of restriction enzymes.

[@avigyanbose5197]

Just keep uploading the videos! There are people who want to learn RDT and Molecular Biology as a whole and you are one of the go to channels that I have ever found on UA-cam! Keep up the good work!

[@theCrux]

I appreciate the kind words :)