Enzymes: Catalysis, Kinetics & Classification - Biochemistry | Lecturio
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- Опубліковано 31 тра 2024
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This video “Enzymes: Catalysis, Kinetics & Classification” is part of the Lecturio course “Enzymes and Enzyme Kinetics” ► WATCH the complete course on lectur.io/enzymesclassification
In this video you'll learn about Types of Enzymatic Reactions, different Bind Substrates, Steps in Catalysis and Kinetics. It'll show you considerations, classifications and parameters. Also it'll focus on Michealis-Menten Kinetics, the Lineweaver Burk Plot and different models like the Concerted Model and the Sequential Model. Lastly there's also a section of different Enzyme Classifications and examples like Lyases or the Isomerization.
► THE PROF:
Kevin Ahern received his B.S. and M.S. degrees from Oklahoma State University, and a Ph.D. in Biochemistry from Oregon State University. Currently he is working as professor of biochemistry and biophysics at Oregon State University. He is also a co-author of the popular biochemistry textbooks, “Biochemistry Free and Easy”.
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Definition and Function of Enzymes
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CHAPTERS:
0:00 - Intro
0:15 - Overview
0:43 - Types of Enzymatic Reactions
1:26 - Enzymes Bind Substrates
6:07 - Steps in Catalysis
6:39 - Enzymes energy, catalyzed vs uncatalyzed reaction
10:05 - Mechanism of enzymatic reaction
11:34 - Background of Serine Proteases
12:19 - Catalytic Mechanism of Serine Proteases
22:57 - Kinetic considerations
30:15 - Michealis-Menten Kinetics, considerations
38:15 - Michealis-Menten Kinetics, parameters
44:34 - Perfect Enzymes, Diffusion Limited Enzymes
47:46 - Lineweaver Burk Plot
49:51 - Catalysis Considerations, Models
53:00 - Concerted Model
55:28 - Sequential Model
57:36 - Binding of Multiple Substrates
1:03:12 - Enzymes Classifiction
1:03:33 - Oxidoreductases
1:04:06 - Transferases
1:04:30 - Hydrolases
1:04:56 - Lyases
1:05:31 - Isomerization
1:06:01 - Ligases
1:06:48 - Outro
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im only in middle school but am very interested in biochem/medicine - thank you for making everything so clear that even a 13 y/o can understand without trouble.
every concept was such a diamond.....crystal clear explanations
really good, made this whole concept much clearer it was only toward the end that it got confusing with the figures displayed
Thank you soo much! I was worried that i wouldn't be able to figure it out on my own.. This helped me out tremendously!
@ around 34:20:
One thing I notice is that [ES] is maximum, and [E] minimum, at the "steady state" point - which seems to mean that the reaction rate there is also maximum. Before that, the reaction is getting up to speed, and after that, winding down. (For me this observation helped bring everything together more clearly). (Very nice video - thanks much!)
a very well professional, organized,explained course thank you so much .
Thank you for sharing this lecture Professor. It simplified enzyme kinetics
Wow... amazing.. i love it... clear and clear ideas... different from books explained... Sir drink water...
Very well explained, thank you for sharing this.
you folks are so good. keep up the good work!!
Well that was amazing, so efficient and easy to understand!
Skip to min 23:00, that's where he focuses on what's important.
Thank you
Is there any way this video can be made more visible to others? I wish I had seen this earlier, so helpful. I have for the first time, grasp the basis for steady-state enzyme kinetics. This video never showed up when I searched a few months ago. Thank God I found it. 👏👏👏👏👏👏👏
very clear explanation, thank you
I enjoyed the lesson sir God bless you
Where were you when I was signing up for classes? Fantastic professor
Damn, I hate biochem but now feels easy.
If you got time then y'all should watch the video..
Question 2: at 30:08 would the relationship be linear or log linear when product is not too high?
Question: how would each step in enzymatic activity reversible if every step has to follow the law of energy change (i.e. one direction should make the total energy higher therefore will not be happening)
my exam is tomorrow wish me luck lol been learning all week but this really helped as a „revision“
It’s an alright lecture, same as in the university
Excellent way sir
world class Professors
Great explanation.
Hello
Thank you sir
7:38 freaked me out.
You are genius ❤️💪🏻
Thank you!!
You saved meeee
You look more formal "professor" than when you were a University professor. Anyways, great in either format.
very good lecture
Thank you!
Hello
Hello, I have a question, and I hope to answer it, please, in the exam. How will the question come to me in Enzyme Kinetics?
Good
you explain it but its still confusing
Frr
he needs time breaking :D. his voices tell me that he's really exhausted
Question: enzymes are basically made up of proteins. What other 2 apart from ribozymes aren’t made up of protein ?
No these are RNAs
Why are proteases termed "serine," while the functional loop of amylases are glycine rich, but arent termed "glycine" enzymes?
Explanation of the basic energetics (minutes 9-10) of the enzymatic reaction is incorrect. An enzyme (like any other catalyst) changes a mechanism of the chemical reaction. In the catalyzed reaction mechanism, different transition states are passed and these have lower activation energies. This should be stated clearly. In the reaction coordinate figure, at least the local minimum of the substrate-enzyme complex is missing!
His objective here is simply to introduce a few basic concepts (Vmax, Km, Vcat, Vcap) in order to help novice students gain an initial foothold in enzyme kinetics. Should he have introduced E+S ES EP -> E+P and the reaction energy diagram for each transition? He could have, and it probably would have been fine. But I'm not sure it would not have helped students toward the lecture objectives: an introductory understanding of Km, Vmax, R-state/T-state, etc.
As instructors, if we inadvertently introduce too many details too fast, we risk confusing students and turning them off. On the other hand, if we simplify at first, we can always add complexity later, as we move forward. Everything in biochemistry is more complex in reality than Chem 100 ever hints at. But Chem 100 gets students started. Everything in biochemistry is more complex than Biochem 400 ever hints at. But Biochem 400 moves students forward. It's a tough, tough call to decide which details to include and which to set aside - in every single lecture and for every reading assignment.
I would say that, in general, chemistry education is quite poor, as evidenced by the very large proportion of students whose number one takeaway from their courses is, "I hate chemistry." That the instructional quality is poor is further evidenced by the appallingly large proportion of chemistry instructors who believe that when students fail to grasp the basic concepts of high school and undergrad courses, the students themselves are to blame ("I teach them the content; if they don't get it, that's on them.") If a teacher can't make students love this stuff, or at least understand and appreciate it, that teacher simply isn't cutting it.
The instructor in this video, Kevin Ahern, proves the potential for instructors to do better. Go watch his live lectures from Oregon State: he gets his students engaged and helps them understand the concepts. An even better instructor, the best I have ever seen in chemistry, in fact, is Tyler DeWitt. His technique and delivery can be applied at virtually every level of chemistry instruction. Check him out. Keep getting better!
Hence proved, Enzyme is a cheater
I hate this type of educational pikeisafish -type narrative. Instead of abstracted story, one should have the concrete experiment that shows how this idea or concept is found - and validated experimentally. The whoever 19 or 20th century person, the experiment, its reagents and primitive limitations. THAT would be real biochemistry.
Am I lost to sea with this idea?
calm down, it's just enzymes.
and thank you for help me 🤍