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Nucleotide Excision Repair in Prokaryotes
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- Опубліковано 16 сер 2024
- In this video we will be discussing about the Nucleotide Excision Repair .
Nucleotide excision repair is a DNA repair mechanism.[2] DNA damage occurs constantly because of chemicals (e.g. intercalating agents), radiation and other mutagens. Three excision repair pathways exist to repair single stranded DNA damage: Nucleotide excision repair (NER), base excision repair (BER), and DNA mismatch repair (MMR). While the BER pathway can recognize specific non-bulky lesions in DNA, it can correct only damaged bases that are removed by specific glycosylases. Similarly, the MMR pathway only targets mismatched Watson-Crick base pairs.
The process of nucleotide excision repair is controlled in Escherichia coli by the UvrABC endonuclease enzyme complex, which consists of four Uvr proteins: UvrA, UvrB, UvrC, and DNA helicase II (sometimes also known as UvrD in this complex). First, a UvrA-UvrB complex scans the DNA, with the UvrA subunit recognizing distortions in the helix, caused for example by pyrimidine dimers. When the complex recognizes such a distortion, the UvrA subunit leaves and an UvrC protein comes in and binds to the UvrB monomer and, hence, forms a new UvrBC dimer. UvrB cleaves a phosphodiester bond 4 nucleotides downstream of the DNA damage, and the UvrC cleaves a phosphodiester bond 8 nucleotides upstream of the DNA damage and created 12 nucleotide excised segment. DNA helicase II (sometimes called UvrD) then comes in and removes the excised segment by actively breaking the hydrogen bonds between the complementary bases. The resultant gap is then filled in using DNA polymerase I and DNA ligase. The basic excision process is very similar in higher cells, but these cells usually involve many more proteins - E.coli is a simple example
Thank you so much for providing the content in such a compact manner. It usually requires hours to just untangle all the information given in books.
Glad it was helpful!.. thanks for appreciation
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Diagrammatic explaination was very impressive and easy to understand
Really very helpful animation, thankyou. God bless you sir
Thank You so much for your amazing works. Can you please explain about protein folding and misfolding. Ramachandran plot and phi psi angles.
Great Explanation Sir ,now it is very much understandable to me
Thank you
thanks for appreciation..Glad it helps ✌️
This is awesome sir, thankyou
thanks for appreciation
Very nice explanation sir ji 👌👌
Thanks and welcome
Keep it up ❤
Great explanation and wonderful presentation as always 💙 #legend
thanks for appreciation..Glad it helps ✌️✌️
great explanation sir ,thank you so much.🤩🤩🤩
thanks for appreciation..Glad it helps....✌️
There isn't a dimer of UvrB right...or is it? ..I'm confused I'm sorry 😅
There is ....
Source : www.ncbi.nlm.nih.gov/pmc/articles/PMC3458569/
nice
thanks Buddy
Amazing