I'm currently working on a method where a pair of hydrophilic peaks elutes in less than 5 minutes, while another pair elutes about 15 minutes later in isocratic mode. When I use gradient methods (~50 to 70% ACN), the baseline drift increases significantly. What is the best way to run a gradient in these methods? Should I implement a steep gradient change in less than a minute or use a smoother gradient (over a longer period) ?
I'm currently working on a method where a pair of hydrophilic peaks elutes in less than 5 minutes, while another pair elutes about 15 minutes later in isocratic mode. When I use gradient methods (~50 to 70% ACN), the baseline drift increases significantly. What is the best way to run a gradient in these methods? Should I implement a steep gradient change in less than a minute or use a smoother gradient (over a longer period) ?