Simplified Online Tools for CRISPR Cas9 Gene Editing Design and Confirmation

Presented By:
Matthew C. Poling
Speaker Biography:
Dr. Matthew Poling earned his Ph.D. in Biomedical Sciences from UC San Diego and joined Thermo Fisher Scientific in 2015. He is currently the lead Product Manager for genome editing technologies and has led the development of the TrueTag Donor DNA system, as well as the TrueDesign Genome Editor online tool. Matt is also responsible for developing and expanding the Invitrogen CRISPR-Cas9 and TALEN technology solutions and works closely with Thermo Fisher's research and development scientists to create innovative genome editing tools and services.
Webinar:
Simplified Online Tools for CRISPR-Cas9 Gene Editing Design and Confirmation
Webinar Abstract:
The versatility of CRISPR-Cas9 has expanded the available toolset of gene editing systems for life scientists. However, these experiments require detailed knowledge of the genomic sequences, both from public reference databases as well as the editing outcomes. Obtaining proper bioinformatics support for either of these steps can be a challenge for both novices and experts. To this end, Thermo Fisher Scientific has built several software solutions to improve the usability of the CRISPR-Cas9 system by addressing two major challenges; the design of gRNAs and corresponding donor sequences, and confirmation of editing success at the sequence level through Sanger sequencing deconvolution.
This seminar will highlight our recent advancements in digital tools, including demonstrations of the Invitrogen TrueDesign Genome Editor online design tool and the Applied Biosystems SeqScreener Gene Edit Confirmation App.
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[@Its_InduB]

What is scoring gRNA targets?

[@gyanaahoduroisaac4598]

Please where can I get the CRISPR-Cas9 software: the toolkit of it. I have been searching in the net for a while but still am not getting the toolkit, so help me out🙏.

[@alicjadownar2076]

I have a question: I need to edit one base in my sequence but this sequence is the sequence of mutated protein so I cannot do that (without mapping gen I can't go to another steps of projecting). What should I do to find sgRNA for SNP of mutated protein (if sequence in not in correct genome). Thank you!!

[@Hansulf]

I mean... You know Its mutated so you know where the mutation is and what It is right? If not... Sequencing is the way to go

[@azukarzuchastux8066]

John 3:16 KJV
For God so loved the world, that he gave his only begotten Son, that whosoever believeth in him should not perish, but have everlasting life.