🔬 002 - How do you make a WET MOUNT for Microscopy?
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- Опубліковано 21 жов 2024
- A wet mount, or temporary mount, uses water as a mounting medium. In this video we will use pond water to make a slide.
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I’m a researcher at UC Berkeley for the department of physics but have been getting into microbiology recently as an interest. I have to say your videos are very helpful!
Your inspiring me to make & view cool things. Nice vid thx mate!!
@Microbehunter : In your comments, you mentioned that "wet mount, or permanent mount, uses water as a mounting medium". A permanent mount doesn't use water specifically as a mounting medium - it uses a mountant such as Canada balsam or another resin (including Numont, L&C, etc), although water may be a solvent for such a mountant (e.g. L&C).
You are correct, thank you for the information. I changed it to temporary mount.
@@Microbehunter You're welcome 🙂
Been wanting to get into wild culture ranching with acetobacteria and yeast, and I've been liking your channel. As a child, I used a miniature microscope to look at macroscopic objects, but the more methodical, proven methods you're using on your channels are very helpful.
Thank you for the feedback. I will be adding more videos.
Microbehunter Glad to hear it.
Thank you! Exactly what I needed!
Excellent presentation. Timing and content just right for an amateur.
I love your simple and effective tutorials. Might I add another method for observing large specimens? Get a ball of soft clay, take your cover slip and gently scrape each corner across the clay to accumulate a tiny bit of clay which will act as little feet and raise the slip when placed on the slide. This was how it was done by my biology 101 lab professor. Cheers!
Another strategy is to put some tape on the sides of the slide and mount the cover slip on it
Do you use distilled water, tap water or rain waters to view your dry specimens? Thank you
Sometimes I do take some water off, but can't help thinking about all the protozoos that might be going off with the water
I can't help but think of "throwing the baby out with the bath water"! Lol
Great video! Thanks for your help.
Thanks for posting this
i would have put the water on the oeter side of the slide because so the alge will swimm more to the center
Sweet! This was extremely helpful. Thank you!
Wow
Superb toturial
Brilliant great upload.
Helpful thank youuu!
Nice ✨
Thank you! Really helpful.
Really good one!
This is much nicer played at 1.75X speed
Are single depression concave slides used for wet-mounting too?
Yes, especially for water fleas and other orgamisms that are larger and which might be squashed by the cover glass. For smaller organisms concave slides are less suitable, because the water organisms swim vertically in and out of focus and it is difficult to track them.
Thank
What microscope can you take right up to the water's edge? (no samples)
One that you would recommend ? (no brand name needed).
Thank you (in advance).
Generally any microscope that is a. sufficiently portable and light and b. has battery-powered LED. You need a so-called field microscope and there are many varieties of them. Google for "field microscope".
@@Microbehunter 👍
can you make a dry mount with an originally wet specimen?
Problem here is that the speicmen might shrink and deform when dry.
I always thought that you were always supposed to use water on slide if you are doing any microscopy
my cover glass has been floating because thy soil pices are too big sometimes
You can stabilize it by making a "bridge". 2 cover glasses (left and right with space in between) hold up the third one.
@@Microbehunter i saw you do that, i ordered a new pipette insted of using they eye dropper. i just got my first microscope
Okay, okay, another superb tutorial. Y'know, a guy could get used to this... ; )
what means wet........do you mean liquid......what solvent...obvious water.....ok everybody knows that water is wet....many thanks for this interesting information
watching this 4 school
Let’s goo hi ms
THIS IS YOUR 2ND VIDEO!!!! I SAW ONLY ELDERLY VIDEOS
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