@Scott Pickering @Scott Pickering in what reality? hopefully the one where actual scientific thinking applies what "basic science" do you figure out what is or is not dna in a pool of stuff? how do you know what breaks down proteins and cells to get at dna? how do you know how to separate dna from other things? can you explain the "basic" science to get at all of the information being presented for each step? So, since its "common materials" it's "basic"? regardless of how many non-"basic" sciences use common materials in a lot of their processes. "its looking at stuff through a microscope".... this is like saying "its looking at stuff using a calculator/computer" for rocket science.... If you just "looked at stuff through a microscope", would you know what you are looking at at all? Keep in mind how he can't verify that these things are indeed dna or not, or if the clumping is from protein or not; he's making educated guesses based on "basic" science? Do you think "astrophysics or rocket science" can't have small inaccurate experiments like this that make things seem "basic" because it's not a "basic science"? How exactly do you define "basic" science, because I don't see a consistent approach being used here.
It's likely an aggregate of supercoiled DNA, the 300nm loops I think would still be way too small. And if you recently ate something especially with starches, you might be seeing a lot of alpha-amylase proteins! We did some similar things in my Biotechnology lab. I think this a great activity btw, it's a really good basic example of DNA extraction without needing purification columns and lab grade detergents
@@tannukashyap8311 Looking back on this you wouldn't be able to actually see the proteins like that, too small. I think I was assuming there is an increase in alpha amylase enzyme production in your mouth during starch consumption to help increase digestion, but I don't actually know if that's true
@@tannukashyap8311 they are enzymes used in the digestion of starch So if you eat a lot of starchy food, then your cells (Intestinal for this one in particular) would respond by synthesizing more alpha amylase enzymes To help in the digestion of it
Najma Samantha...Microscopy, and amateur astronomy are the best hobbies anyone can get into!..It sure beats coin, and stamp collecting which I find to be dull, and boring!
@@damontsekos365 hobbies are subjective. I find stamp and coin collecting boring as well but you can’t go around telling people what they should be more interested in
I am sure you can do fluorescence microscopy . You could use a DNA fluorochrome stain such as Acridine Orange to stain the DNA. Alternatives are natural DNA staining fluorochromes include Berberine (from Barberry - Berberis spp.), and extracts from Greater Celandine (Chelidonium majus) which is a common plant in many parts of the world, and Plume Poppy (German = Federmohn) (Macleaya sp). You don't specifically need UV for this fluorescence, because most of these stains can be excited with Blue light and emit a yellow gold color.
Must admit the title and thumbmail got me it - "look at my DNA under the microscope" and the head of Microbehunter - I'm thinking, wrong website/hub. But no, it's educational, what a surprise.
Im learning a lot more with your videos than with my classes at the university. I've seen your gallery, it's fantastic, I use the pictures to compare what Im looking in the microscope. Your job is amazing
point of classes at university is to give you the background to allow you to get to these types of things on your own, designing and figuring things out. just watching and learning specific things is mostly only beneficial for a particular job or task. it can be more motivating because its more interesting, but it should motivate you to learn the class stuff.
you should've used eye drops as they have cellulase and amylase and starch would be destroyed, more chances of actually seeing DNA. although i think the micrographs you got at the end, the little beads on string are actually DNA. you madman Oliver I love you you did it
Sir you could use lens cleaner as protease to degrade proteins (histone) that binds up dna and see in micro scope of high resolution you may get a better view of dna
Most excellent spit sample microscopy analysis. Whether or not we could see dna is compensated by impressive discussion of observed micro forms and structures. Bravo!
I had experienced and did many experiments in the high equipped lab while doing my post graduation in microbiology discipline... ! My researches included microbiology, biotechnology, biochemistry,cell biology and molecular biology... !
You have the best channel on microscopy, but you need more videos about vintage microscopes, vintage (and modern) staining methods, and diy staining (there's something on the internet, but I only believe in your recipes 😉).
I don't have my microscope yet. It will be here Wednesday. But I still find y our videos fascinating. I had no idea one could isolate DNA like this. I guess I better start saving up for my very own Scanning Electron Microscope. :)
I still wonder if my science teachers were this amazing ..I would have choosen science for rest of my life... Though with no regrets ... Being a commerce graduate...I still carry my interest for science experiments. 😀
Dishwashing solution. A non ironic surficant dissolves away the fatty acid in the cell membrane and lets the contents spill out. Great for disinfecting also
DNA reacts with diphenylamine based on the conversion of deoxyribose sugar in the DNA molecule (not in RNA), so when hydrolyzed at high temperature in an acidic environment to form hydroxylevulinyl aldehyde which reacts with diphenylamine to form a blue complex sustainable construction. So you can do this extra step to identify colored DNA when observed under a microscope and see :)
I was thinking to do this experiment 🔥😎 But when I told my father to bring some alcohol for me he was started to explaining me that how a man can get difficulties after taking alcohol and stuff😂😂😂😂 (I didn't tell him that why i am demanding for it)😂😂😂
I know that the DNA, which is an acronym for De-oxy-ribo-Nucleic-Acid, is a double helix structure with some tiny steps and phosphate sugars on it but, I don't think I can actually see those things under my personal microscope. What is the name of the detergent you made use of? Is it Persil, Tide or Gain?? Or did you make use of any common detergent? I'll try this experiment once again and make sure I get the right viewing under the microscope. BTW, this video was amazing! Thanks for sharing.🎆🎆😁😁😊
Incredible video! Seeing any amount of DNA under a light microscope of all things is far better than nothing at all. Thank you for the tip. However, can you go more into detail about this procedure? Never heard of this before and not too sure about the materials, for example if it is better to use 99% isopropyl alcohol or something much lower like 70%. Many Thanks again!
I have used 96% (about) ethyl alcohol. It works better when the alcohol is ice cold, but I did not cool it and it also worked. The DNA participation with alcohol is standard procedure in molecular biology. The alcohol reduces the polarity of the water to the extent that DNA can not be dissolved anymore. Normally one would collect the DNA by centrifugation after the addition of alcohol.
Thank you so much for doing this video. I found it exceptionally engrossing. I appreciate learning how scientists make the "DNA" strands they can take out of the tube. I've always wondered about that. Bravo!! 👏
I’m a cosmetology teacher and I have thoroughly enjoyed this. I want to find a microscope with proper lenses and magnification to view hair at its cellular level. I’d also like to be able to view things like hair color’s molecular structure. Any ideas on which strengths I may need?
It's an Olympus BX53 with Planfluorite objectives (4x 10x 20x 40x 100x oil) and Differential Interference Contrast (DIC), which is expensive but produces very nice images. You need a separate prism for each objective and they need to be fit in into a condenser with a rotating turret. I obtained several offers, all similar in the price range, some even more expensive.
Hello, I’m currently a freshman starting veterinary medicine and I’ve been looking for information on how “far” a microscope can let us observe. My question is, and I would appreciate if you help me answer it, could one observe the DNA of an animal thru the microscope? Other than bacteria and cells, what else can a microscope let us observe? Please and thank you.
Just tried it, ended up with a white/gray goo that I tried to spread 'under' my inverted microscope. Think I need to first alter my dna to get a third hand to hold everything in place while I look at it 😂
Not only is the video cool, but the micrograph website is really cool! Could this be done with a cheap educational microscope? Like a normal 1000x compound scope? Keep in mind I know NOTHING about chemistry and little about DNA, but could this be mucus or something? It certainly acts like it, but I dont know.
hypothesis: those strings/nets could as well be microtubules, or even recombining cell wall proteins. i do not trust alcohol to preserve long dna chains and certainly not bleach. maybe we should try this experiment by centrifuge
Alcohol does preserve DNA, Alcohol precipitation is the standard method for DNA isolation. Beach was not added, its oxidizing ability would indeed destroy DNA.
How it possible to see a small molecule to under the compound microscope because the resolution is 0.3 micrometer and magnification power is 1500x while the size of DNA molecules is 0.6 manometer.
This is the type of Science I wanted to learn in school
You have to take genetic engineering as a career
Me too, now im grade 10 we are learning about gender 127
Exactly you are right I am in class 11 now
@Scott Pickering how is this "basic" science? because he made it seem easy using common materials?
@Scott Pickering
@Scott Pickering
in what reality? hopefully the one where actual scientific thinking applies
what "basic science" do you figure out what is or is not dna in a pool of stuff? how do you know what breaks down proteins and cells to get at dna? how do you know how to separate dna from other things? can you explain the "basic" science to get at all of the information being presented for each step?
So, since its "common materials" it's "basic"? regardless of how many non-"basic" sciences use common materials in a lot of their processes.
"its looking at stuff through a microscope".... this is like saying "its looking at stuff using a calculator/computer" for rocket science....
If you just "looked at stuff through a microscope", would you know what you are looking at at all?
Keep in mind how he can't verify that these things are indeed dna or not, or if the clumping is from protein or not; he's making educated guesses based on "basic" science?
Do you think "astrophysics or rocket science" can't have small inaccurate experiments like this that make things seem "basic" because it's not a "basic science"?
How exactly do you define "basic" science, because I don't see a consistent approach being used here.
It's likely an aggregate of supercoiled DNA, the 300nm loops I think would still be way too small. And if you recently ate something especially with starches, you might be seeing a lot of alpha-amylase proteins! We did some similar things in my Biotechnology lab.
I think this a great activity btw, it's a really good basic example of DNA extraction without needing purification columns and lab grade detergents
It could be condensed chromatin fibers, which is roughly 700 nm wide. That could be seen with a light microscope I think.
why do we see alpha-amylase proteins after eating starch?
@@tannukashyap8311 Looking back on this you wouldn't be able to actually see the proteins like that, too small. I think I was assuming there is an increase in alpha amylase enzyme production in your mouth during starch consumption to help increase digestion, but I don't actually know if that's true
@@SystemUpdate310 I think it's possible to see 700nm in a light, and yes it is condensed chromatin fibers, heterochromatin is 700
@@tannukashyap8311 they are enzymes used in the digestion of starch
So if you eat a lot of starchy food, then your cells (Intestinal for this one in particular) would respond by synthesizing more alpha amylase enzymes
To help in the digestion of it
Amazing!!! I wish more people got interesested in content like this!!!!! Just fascinating, thank you very much
Totally magnificent dear
Exactly :)) people should show their more interest in science ...
Najma Samantha...Microscopy, and amateur astronomy are the best hobbies anyone can get into!..It sure beats coin, and stamp collecting which I find to be dull, and boring!
@@damontsekos365 hobbies are subjective. I find stamp and coin collecting boring as well but you can’t go around telling people what they should be more interested in
People are suprised that I’m 11 and I like science and not stupid video games
Such a genuine content .
Not the cringe as other creator
I wish this kind of proffesor are also present in pur college🌸🌸
Hai Apoorva were is our college in India
Academia has an agenda ,,,keep them dumb ,,,,not too dumb.Evil to the lying core.
I am sure you can do fluorescence microscopy . You could use a DNA fluorochrome stain such as Acridine Orange to stain the DNA. Alternatives are natural DNA staining fluorochromes include Berberine (from Barberry - Berberis spp.), and extracts from Greater Celandine (Chelidonium majus) which is a common plant in many parts of the world, and Plume Poppy (German = Federmohn) (Macleaya sp). You don't specifically need UV for this fluorescence, because most of these stains can be excited with Blue light and emit a yellow gold color.
I am a young and new passionate science teacher! Glad I found your channel! I love learning the content you produce Sir
The open source licensing s very honorable. Thank you very much!
Absolutely!! 100%
Must admit the title and thumbmail got me it - "look at my DNA under the microscope" and the head of Microbehunter - I'm thinking, wrong website/hub. But no, it's educational, what a surprise.
Im learning a lot more with your videos than with my classes at the university. I've seen your gallery, it's fantastic, I use the pictures to compare what Im looking in the microscope. Your job is amazing
point of classes at university is to give you the background to allow you to get to these types of things on your own, designing and figuring things out.
just watching and learning specific things is mostly only beneficial for a particular job or task. it can be more motivating because its more interesting, but it should motivate you to learn the class stuff.
Then you must not be paying attention in your university classes...
you should've used eye drops as they have cellulase and amylase and starch would be destroyed, more chances of actually seeing DNA. although i think the micrographs you got at the end, the little beads on string are actually DNA. you madman Oliver I love you you did it
Sir you could use lens cleaner as protease to degrade proteins (histone) that binds up dna and see in micro scope of high resolution you may get a better view of dna
Sir in a very beautiful way you have shown DNA. It is really fascinating.
I just downloaded a PDF on doing the banana activity. Going to do it with my grandchildren. It was great to see how all was done, Thanks
Most excellent spit sample microscopy analysis. Whether or not we could see dna is compensated by impressive discussion of observed micro forms and structures. Bravo!
I had experienced and did many experiments in the high equipped lab while doing my post graduation in microbiology discipline... !
My researches included microbiology, biotechnology, biochemistry,cell biology and molecular biology... !
So you would say it is DNA that we are seeing?
I study b SC biotechnology 1st year. In my practical we do Isolation of DNA from both plant and animal tissue. it's gave a best results.
The wood also good in case any of the DNA happens to be vampiric.
You have the best channel on microscopy, but you need more videos about vintage microscopes, vintage (and modern) staining methods, and diy staining (there's something on the internet, but I only believe in your recipes 😉).
The sound when he spit out salty water was satisfying 🙏👍👍😂😂
I tried it and it worked!...Thanks!
Man, it's awesome. Keep up the good work 👍🏻
I don't have my microscope yet. It will be here Wednesday. But I still find y our videos fascinating. I had no idea one could isolate DNA like this. I guess I better start saving up for my very own Scanning Electron Microscope. :)
The laugh after “wood is good” XD i actually died
what kind of microscope is neccessary to really see the DNA?
Great day for me to see DNA live. Thank you.
O my God! that was such an amazing experiment oliver.....Thank u so much..
Wow excellently cool experiment
You are an amazing person,
This is my first video of your
woah. That’s cool. Can’t wait for more content!
I love your good vibes. TY for the teachings. Much love.
I still wonder if my science teachers were this amazing ..I would have choosen science for rest of my life...
Though with no regrets ... Being a commerce graduate...I still carry my interest for science experiments. 😀
Not too late my friend, sit down, make plans and go for it, live while you are alive.
It has a beads on string structure so probably is chromatin material.
yeah i agree
I've done too at my school! Although the result was different from yours , but it made me really excited😍
Dishwashing solution. A non ironic surficant dissolves away the fatty acid in the cell membrane and lets the contents spill out. Great for disinfecting also
wow i was just asking myself where would i be able to find library of these mico friends .Thanks alot!
me too mate.
DNA reacts with diphenylamine based on the conversion of deoxyribose sugar in the DNA molecule (not in RNA), so when hydrolyzed at high temperature in an acidic environment to form hydroxylevulinyl aldehyde which reacts with diphenylamine to form a blue complex sustainable construction. So you can do this extra step to identify colored DNA when observed under a microscope and see :)
Thank you so much sir for this great work.I was really excited to see DNA . Love from INDIA🇮🇳 ❤️🇮🇳
I love the moment you pull your saliva from the measuring cup and call it a clump of DNA :D
Awesome experiment sir 🙂.Thank you for sharing.
Bro it's awesome and you are a good content creator
I was thinking to do this experiment 🔥😎
But when I told my father to bring some alcohol for me he was started to explaining me that how a man can get difficulties after taking alcohol and stuff😂😂😂😂
(I didn't tell him that why i am demanding for it)😂😂😂
tell him then
Sign up for the Newsletter: www.microbehunter.com/newsletter-signup/
Followup Video - Better DNA extraction: ua-cam.com/video/3qvm3w2zwgA/v-deo.html
I know that the DNA, which is an acronym for De-oxy-ribo-Nucleic-Acid, is a double helix structure with some tiny steps and phosphate sugars on it but, I don't think I can actually see those things under my personal microscope. What is the name of the detergent you made use of? Is it Persil, Tide or Gain?? Or did you make use of any common detergent? I'll try this experiment once again and make sure I get the right viewing under the microscope. BTW, this video was amazing! Thanks for sharing.🎆🎆😁😁😊
This is f ‘ing awesome holly crap
Amazing as always. Thank you for all you do. This is my favorite video I have seen.
Thank you for creating this image database!
Incredible video! Seeing any amount of DNA under a light microscope of all things is far better than nothing at all. Thank you for the tip.
However, can you go more into detail about this procedure? Never heard of this before and not too sure about the materials, for example if it is better to use 99% isopropyl alcohol or something much lower like 70%.
Many Thanks again!
I have used 96% (about) ethyl alcohol. It works better when the alcohol is ice cold, but I did not cool it and it also worked. The DNA participation with alcohol is standard procedure in molecular biology. The alcohol reduces the polarity of the water to the extent that DNA can not be dissolved anymore. Normally one would collect the DNA by centrifugation after the addition of alcohol.
Can you make a video of mushroom cells under a microscope?
Thank you so much for doing this video. I found it exceptionally engrossing. I appreciate learning how scientists make the "DNA" strands they can take out of the tube. I've always wondered about that. Bravo!! 👏
Wow how smart... Is the rounds things cells
I hope....I can find a teacher like you👏
You are terrific! I love your presentation and the information you bring us. Two thumbs up!👍 👍
I really enjoy seeing microbes. I am glad to have found your channel. Thank you!
This is the real science .. Love it
Hey, it's been about three months since you posted this video. Any updates? did you confirm it was DNA?
Good question
It is DNA
@@МиленаКамчева Лаборантка? :)
Thanks for the upload...
Wow 🤩 I like the colourful microscopic background stunning 😍 thank you for sharing
Great video again
Loved it!!
How cool! Thank you for sharing!
That sound effect the moment he spit the saltwater though 😏
Perhaps a silly question. Shouldn't the utensils and equipment be sterile?
I had to do so much scrolling to find this video! Thank you!
it would be great if you could include the magnification and ledger in the photos. very entertaining. TY
Microbehunter you may use pipette, syringe, medicine dropper either 😊 .
Nice video.
Why best channels are underrated?!
Any INDIAN watching this🙌🙌🙋....😊☺
🇱🇰❤
Yes! I'm!
😅
A am
Please make a video on how to match two dna
Very interesting.
Thank you.
With good education everyone can be a doctor or related field.
Ok. Here I see someone teaching microscope. Here I am. An enthusiast 🥸
How do you know it is DNA and not bits of salt, washing up liquid, old food bits, saliva or water etc?
i did it mr. oliver kim thk u
helped on my project
I’m a cosmetology teacher and I have thoroughly enjoyed this. I want to find a microscope with proper lenses and magnification to view hair at its cellular level. I’d also like to be able to view things like hair color’s molecular structure. Any ideas on which strengths I may need?
There are many options. You might want to have a look at my other channel for help: www.youtube.com/@microbehuntermicroscopy
Just discovered the channel and subscribed. Very nice content!
Sir plz show some solid elements powder or stuff like that in a microscope..
I looked at my DNA today. It looks just like yours... Dad?
I love your channel and hope to donate soon!
Thank you so much!
Oooo i can believe that there are so genius UA-camrs like u ....this what i like to see ...i subscribed u i liked u..thank u sir
I leant something new and feel good. 👍
i heart you, microbehunter.
every I see your video, I remembered Prof. Punshuk Wangdu from 3 idiots film
Indians...me too.
Good one! Great topic. What kind of microscope are you fundraising for? I want to read the specs. :)
It's an Olympus BX53 with Planfluorite objectives (4x 10x 20x 40x 100x oil) and Differential Interference Contrast (DIC), which is expensive but produces very nice images. You need a separate prism for each objective and they need to be fit in into a condenser with a rotating turret. I obtained several offers, all similar in the price range, some even more expensive.
@@Microbehunter OoO an Olympus! I hope to be able to donate too!
Hello, I’m currently a freshman starting veterinary medicine and I’ve been looking for information on how “far” a microscope can let us observe. My question is, and I would appreciate if you help me answer it, could one observe the DNA of an animal thru the microscope? Other than bacteria and cells, what else can a microscope let us observe? Please and thank you.
The video was interesting keep it up good work!
That microscope needs steady hands and lots of patience. A high school grad doesn't have both 😂😂
Just tried it, ended up with a white/gray goo that I tried to spread 'under' my inverted microscope.
Think I need to first alter my dna to get a third hand to hold everything in place while I look at it 😂
Can't spit ants into tt?
You need a sugar saluting to open up cells
First time here ,excellent . Check a Mudfossil for DNA would be cool.
😁 I really like the Microworld Archive idea! Which microscope and camera are you aiming for?
Not only is the video cool, but the micrograph website is really cool!
Could this be done with a cheap educational microscope? Like a normal 1000x compound scope?
Keep in mind I know NOTHING about chemistry and little about DNA, but could this be mucus or something? It certainly acts like it, but I dont know.
The microscope must be good and I also used phase contrast. If the microscope has a proper condenser, then maybe yes.
Great video!
hypothesis: those strings/nets could as well be microtubules, or even recombining cell wall proteins. i do not trust alcohol to preserve long dna chains and certainly not bleach.
maybe we should try this experiment by centrifuge
Alcohol does preserve DNA, Alcohol precipitation is the standard method for DNA isolation. Beach was not added, its oxidizing ability would indeed destroy DNA.
Hi, this is really interesting. What if we try and see bacteria in that solution? Would this be possible?
You are very humble
GOOD LECTURE 👍 GOD BLESS YOU TEACHER 😊
It was a very interesting project
Amazing try buddy thank you so much so real
What microscope did You use that must be very powerful Is it by olympus?!
I am using an Olympus CH-40 and an Olympus CHA with phase contrast. Other microscopes should also work.
Can you please give me the measurements of all the things that you used? I tried it but nothing happened so I think my measurements were off.
I'm trying to do a project on this...your support would be very helpful!
I did not measure anything, i estimated. Spoon of salt in 100ml water, drop of liquid soap, 96% ethanol.
@@Microbehunter ok thanks so much!
How it possible to see a small molecule to under the compound microscope because the resolution is 0.3 micrometer and magnification power is 1500x while the size of DNA molecules is 0.6 manometer.
Tooo...... Interesting... 👌
Can u do a study on the plasma membrane f human rbc
Sir can we use sanitizer instead of Isopropyl alcohol