How to prepare plant extracts using various solvents||Methanolic extract
Вставка
- Опубліковано 29 сер 2020
- Learn how to prepare various plant extracts using different solvents for biochemical assays, HPLC, GC-MS studies etc.
Do watch the biochemical assays in my other videos to know how to analyze the proteins, carbohydrates, proline, phenolics, flavonoids and many more...
Do share and subscribe!
Thank you!
That was so informative thank you so much. I was struggling with this to understand through paper for so long and your video cleared everything
Please keep posting more videos regarding phytochemical analysis of the plant and also about essential oils of the plants.
Very much clear and informative
Thank you SIR ! Much needed video.
Thnkyou so much sir for your effort....your videos are very beneficial ......
It is so useful sir
Thanks 👍
Thankyou . Its quite helpful.
very clear explaination...👌👌🙏👍👍
Your video is extremely informative Sir. Thank you so much 🙏
:)
I am following your videos in my PhD work
Bro can you tell me the reference he's is using
Very nice👌
Thanks a lot sir 🤝❤️
Very informative video
Gr8 video sir
Sir very good job done by you kindly share videos related to bacterial research related to metal NPs and ethyl acetate extraction of bacterial metabolites
Well explained Rakesh...
Thank you mam 🙏
Thank you for the video! My project is to identify the best brewing temperature and time for green tea leaves to exhibit the most antioxidant capacity. Can I directly use the sample ( solution of tea and water) to carry out DPPH essay?
Thank you brother ☺️
Thankyou, it helped me quite alot.😃
Happy its helping:)
Thank you so much sir
Very informative video sir. Thank you for uploading. I have a plant whose phytoconstituents are not known. M planning for tlc bioautography can you help me how to select solvent system?
It was very informative... sir I have a question... after evaporation I have the extract in pasty form can I dilute it in the same way as u have shared in ur video?
Thank you for the clear explanation. I have a question- after methanol extraction and evaporating the methanol, when you re-dissolve the plant residue, can you use any solvent other than methanol? Can I re-dissolve the residue in DMSO to make 1 mg/mL extract in DMSO? Will it dissolve in DMSO? For my experiment I cannot use methanol. It must be DMSO that's why I am asking. Thanks.
Sir wonderful explanation i have a doubt which analysis is best to test the phytochemicals in plant extract? Is GCMS is good?
Killler
Absolutely brilliant
Thank you 🙏
Thank you so much. Can you share plant extract preparation in different solvent for GCMS analysis, so that I can send the sample for analysis to other lab. Thanks again.
Sir you are great.
Means a lot. Thank you :)
Super sir
Hello sir, can i use freeze drying to evaporate the methanol after filtration?
Thank you Sir. How will I know how much methanol will I put to my extracted plant?
Hi sir
Just need to know one thing, I have done serial plant extraction using pet ether, chcl3, and ethanol.. so will u plz tell me how can I make 1mg/ml solution of chcl3 and pet ether extract for TFC TAc, etc. Like which solvent I can use to dissolve these solvent extract for my further analysis
Hi sir, lets say i have prepared my methanolic extract for 1mg/ml, i want to further dilute it for some range of concentrations, can i use distilled water to dilute ? this is especially for total phenolic content.
nice video, i have a doubt, i have small amount of sample, can i use four different solvents one after another on the same sample?
Thank you for the video, sir. I was wondering what concentration of extract (in mg/mL) is needed for the DPPH assay? Thanks!
Yes sir i have the same question
What happens if the fresh sample is taken (eg; Fresh roots) to prepare methonolic extract? Will it affect the results?
Good effort, but falls short.
Sir then for the standard solution i have to prepare 1mglml ?
Nice
Sir, Can I use this for plant protein extraction before using ultrasonication?
Sir this protocol is same for fruits samples
Is the method can be used for other sample? Means other than plant samples
I dried my Methanolic, acetone and aqueous solvent at 45° in oven for 3?? 4 days. Is my sample can't be used for phenol estimation
Sir I have a doubt . I was asked to take the quantity of the sample to be dissolved in the solvent according to the concentration of the sample. Can you tell me how to check whether the sample is concentrated ? Is it by keeping the sample in the solvent i.e; 1:10 ratio overnight and if the solvent evaporates to 3/4 th it means my sample is concentrated Sir?
I need the cellulose contents in gel or viscous form , so is there any method to get gel sample?
Is this same for water extraction also?
How long we use crude extract for further activity
hello, what do we need to preserve the extracts if we will use only for a few days? is anything necessary to add or no need?
Sir for our project we need to prepare the methanolic extract of aloe vera gel. Can you please guide us on how to proceed for it.
How to purify the extract obtained like fractionation and column chromatography please explain. Thanq in advance
if it is oil essential or fixed oil then how to dilute in small amount sir
And my doubts are cleared now , thank you
Sir, is it necessary for also vit c quantification ?
Sir I have performed extraction of plant in microwave by using methanol solvent and I have to prepare methanolic extract to detect flavonoids on tlc plate please suggest me how to do that
What if I want the methanol extract for my study, what will be the procedure?
Sir plz reply how to use fresh sample for flavonoid content and phenolic content determination
Does this particular method for extract preparation have a name?
Thank you for easy explanation of procedures.
Sir, could you please provide a reference for this procedure... ?
Thank you
Usually no paper provides or explain this as it's a well known basic procedure. Not discovered by any particular person to give reference. You can watch my video on references and download my paper and cite the same
good morning sir
i want to know about plant extract for antimicrobial activities .....because this is my research topic ..plz guide me ...
Can one do vacuum (room temperature or lower) drying.
Thank you for your video :). i will be extracting peel, how to choose between ethanol or methanol as solvent?
Go with methanol
Sir i m doing phytoremediation of BPA.. Nd for extraction after drying i grind the sample with buffer nd aftr that i put methanol. Will it hamper in any way?
Hello sir,may i know how to estimate the concentration of plant extract using UV double beam spectrophotometer?
Hello Sir, I am doing an experiment on a ethanol plant extract that I bought from a well known company. I want to check its antibacterial actions against some bacterial strains. I found no literature about the preparation of the solution of the commercially available ethanol extract of the plant. Therefore, could you please guide me as how to make the solution to check the antibacterial effects of the extract? Thanks
very very useful video...sir will you please tell..for extraction how many solvents we can take ideally?
It depends on the work, is it Ph.D or Msc project. Ideally we take 2 polar solvents and 2 non polar solvents. You can increase if you have time and interest.
Can you please explain the final concentration of extract...Please provide a calculation of the final concentration of extract.
Thank you for the video sir. In my case I have to extract the chlorophyll-a from algae, so do I have to store the filter paper with solvent for 24 hours in the refrigerator before grinding it or should I have to grind and then store it in the refrigerator
Chlorophyll estimation is done by arnons method using 80% chilled acetone..Browse with this keywords you will find papers or in one video in my channel theres a video about references. Watch that video and download my paper for reference
How much time do we keep on oven?
thank you for your clear explanation
Please make a video on work mechanisms of rotary evaporator? And is there any simple method to remove solvent?
Sir j plant extract bny h vo 20microlitre m hi hoga ky ml m nhi hoga ky
sir , My topic of research is to determine the invitro antidiabetic properties of the medicinal plants. A humble request to you to make a video on this assay.I always watch your videos before performing assays.plz make the video as soon as possible .
Im not working in lab. Im afraid that i can't be doing any experiments for a while.
sir as you perform dpph and other assay so i told you for this bcz these videos helps me alot
Hi! Can you also tell me how can I obtain the chloroform or ethyl acetate fraction from the lyophilised ethanolic extract of my plant?
Redissolve it in whichever solvent you want and follow the same steps
Thanku so much sir, sir can u plz make video for determination of stomatal number .
Ok :)
And redissolving with methanol, is there any important in phytochemical constitution.
Sir, thank you so much for your information. I have planned animal studies, after getting dried extract, redissolved in water and give it to the animal. Is it right sir.
@@Dr.Rakesh.B thank you sir
Sir plz make more videos on phd experiment
Sir
I am a pg student
For my thesis work which is on herbal extracts , i need some help from you.
Can u please guide me sir
I need to qualify and quantify the phytochemical in my extract so which chromatography is good for me.
@@Dr.Rakesh.B thank you so much
Sir can i use the fresh plant material for methanolic extract . For eg can i homogenise the sample directly in methanol, centrifuge amd use the supernatant for DPPH assays
Yes
Sir kindly sahre information to remove chlorophyll which is intefering in HRMS with untargeted meatbolite estimation.
Bro pls explain how to make mother tincture ?
Hello Sir, I question is. For Total Flavnoid and Phenol estimation can't we use the direct extract instead of evaporating? Plz answer
U can use but the conc.wont be accurate
How do you store powder?
I was planning to put it in a ziplock bag. Shall I store it in refrigerator or room temperature?
Ziplock will do or any air tight vials and refrigerator (freezer).
Do we have to add methanol again to powder which we extracted previously with methanol?
@@Dr.Rakesh.B thank you
Are these extracts can be consumed by normal person?
Sir in hot air oven up to how much time I should keep the extract for further evaporation after rotavap n also tell plz that is dryer oven n hot air is same...
As long as you can regulate the temperature any oven is fine. Keep for 2-3 days at 40 degrees
Hi sir, I have a question did different amount of leaves produce different amount of extract?
@@Dr.Rakesh.B Yes sir. That's what I mean. Thank you so much sir
Sir for the extraction of saussurea lappa , is methanol or ethanol is good?
Please read literatures related to it.
How to take extraction from euphorbia plant
Sir how can we prepare extract sample of juice for antioxidant examination
Thank you for response sir.
I am not actually trying for commercial juice. It's a blend of ginger and basil leave juice extract with no preservatives. I think in that case I can take directly juice as sample (as you said)?
good evening sir
sir can we perform antibacterial activity of plant powder without any extraction if it is possible kindly guide me
i m working on a herbo mineral preparation for my dissertation work
No, you have to dissolve it in some solvent .
Can we take fresh leaf samples for estimation of SOD MBD and catalase estimation
Yes
sir could you please mention the article for references purpose. thank you
I have made a separate video for references pls watch it
Nice video sir, 1f 10 mg is required for 10 ml methanol, how much methanol will be required for 0.32 mg
320mL
How much percentage of ethanol is used in this process
Can you please tell me the research papers which you followed make these videos like Preparation of plant extract, DPPH, TPC
There's a separate video in my channel for references. Please Watch that
Sir we want to know hplc with plant extract
Sir same extract is used for antimicrobial activity?
Yes you can use
Sir how can I mail you? I want to ask about one plant extract preparation for my master’s thesis sir
Can i use normal filter paper ?
Yes