Instant Pot Sterilization of Rye Grain for Spawn - Home Mushroom Growing - How To Procedure
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- Опубліковано 1 тра 2022
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After rejecting the instant pot for sterilization method for years, I finally decided to give it a go. If you watched my last video ( • Grain Spawn in the Ins... ), you saw that it is definitely possible to successfully sterilize rye grain in an instant pot! After tinkering with the procedure for a bit, I decided I'd share this how-to video. If you'd like to follow along with the written procedure, it's located here: oneearthmushrooms.com/pages/g...
The point of soaking the grain over night is to allow certain organisms to germinate, these organisms while in spore form can tolerate 15psi, at 90 min but once germinated they cannot.
Thank you for the piece of information, Iv just did my first grain pasteurizing and soaked it for 24 hrs following shroomy guidelines, didn’t know why till you mentioned it, will do that from here on out as it makes sense.
@@irvvvv601 u know that's total bullshut what he just said. People like making extra unnecessary steps and calling it endospores. Everything dies once pc reaches a given Celsius nothing can survive thats the whole point of pressure cooking. This what he's mentioning is a old man's tale. This is why we call it sterile.
I’ve had more problems with splitting grains causing contamination. I’ve been doing
No soak no simmer for a while now and if I don’t get split grains I’m less likely to get contamination than from the 24hr soak prior to cooking
It'd be a cool test for someone, 3 soak 3 no soak, examine results.
Smart putting the lids on the base of the pot, I never thought!
I can verify that this method works perfectly! Thanks!
Thank you, Joshua!
@@OneEarthMushrooms I'm near pinning using this grain spawn method and your monotub method using the substrate calculator. Thanks so much for you work, it makes everything easier.
Awesome to see an instant pot in use!
Holy cow brother. If I can make rice in my Instant Pot, I can sure wing this. Gourmet mushies coming our way. Thanks brother.
A couple additional notes on using an Instant Pot for sterilization...which I love. It is important to understand that altitude impacts the max pressure these devices can attain. Some manufactures compensate for this, but the info is rarely published. This can help account for the varied experiences people have with these pots. Instead of guessing, a $6 roll of autoclave tape will tell you if you are getting the temperatures you expect. While the tape is generally used to measure the outside of a container and cannot guarantee sterilization of media, it can be used to determine a) if you get to sterilization temp, and b) how quickly you get to that temp (using some test runs) This can inform how long you need to let grain bags or agar cook with your pot at your altitude. I'm at 6500 feet and began wondering if my 6 qt. Instant Pot Duo (that I use for everything mycology) was hitting the temps I expected. The tape confirmed that I could trust the pot. I've not tried to insert tape strips into the center of test bags yet, but a quick Copilot chat tells me this is a valid protocol.
Thank you so much!!! I dread hauling out the huge 23 qt PC for just and then feeling the need to fill it completely or Im wasting a whole PC!!! So this is so Awesome! I thought all I could do in my insta pot was agar and liquid culture! So now I can finally go at a more day to day pace with my grain spawn!! Thank you again so much!!!
I am grateful for this video. Thank you!
As many people have said this definitely works. My spawn jars were in 30+ degrees Celsius where contam is even more likely for over 2 weeks. Works great
I live in tropical country where my room can reach 30c during hot days. This brings relieve to me.
I was literally planning to do this today!
Thanks! Simple and effective!
Thank you. I've been avoiding grain and just biting the bullet of pre-made grain bags, but it's been 50/50 on if they colonize at all... this is simple enough to do, and should make the failures a lot less costly at least
Awesome thank you!!
I will try this even thought I do have a pressure cooker
Hey this is a great video people should realize that instant pot really is just a small pressure cooker and I really don't think you need to soak the grain overnight because that kind of defeats the whole purpose of the instant pot. of it if you're gonna cook Pinto beans and other things on the stove you soak them overnight but having an instant pot means that you can throw dry beans and water right in the pot and you're good to go this applies the same thing for the grains you're prepping for the jars.
he soaked overnight to germinate endospores... at least this is the logic of many growers. Personally I think you could cook boil to grain and dry them out and let them sit for 24 hours in their jars prior to PCing. I assume the endospores will begin germination within 24 hrs after a boil. But I have no evidence to support this.
its to deal with endospores. You need to presoak so they get killed in the pc process. don't skip the soaking.
@@MosesMLam lol biggest crock of shit i bet you just heard that somewhere and mentioned it here. U people love putting extra unnecessary steps. Spores or endospores as you call them all die anyway when u pressure cook thats the whole point of cooking this way. No need to endospore it 🤣
I came across an even simpler method on Shroomery. It comes down to using the exact amount of water for the rye. Put 2/3 cup of rye berries and 3/4 cup of water directly in the jar, seal it and sterilize it in Instant Pot (I use the IP's Sterilize program for 35 minutes). Done! I've been using this process successfully for a few months now.
Are the lids modified in the method you found? GE filter, foil, injection port?
@@HelpMe_OrNot For grain jars I make lids with only the injection port and a simple GE filter disks. For Liquid Culture lids, I use the bigger plastic "injection ports" with built-in GE filters. I don't use it for injection (the mycelium wouldn't make it through the filter) but they are more resilient to contam by liquid than the little filter discs. The grain jars colonize within 5-7 days for most Oysters but a jar of Pink Oyster last week took only 4 days to colonize!!
I use an instant pot also, I do three hours just in case. Never had any contam
Did my first batch yesterday, yup 3 hours Iv heard it almost fail proof
Thanks I was wondering how long to sterilize. I am trying corn
Would you recommend rinsing the rye berries before soaking in water + gypsum?
Thanks...great to see. Was wondering my comment referencing a relevant (and supportive) Instant Pot laboratory study was nuked from this video. Your channel your rules, I get it, just trying to understand them. Cheers
Hi there! I haven't seen your comment. I checked comments held for review and don't see anything from you. I'm wondering if UA-cam autodeleted it for some reason...maybe because it had a link? Try responding to this comment with the study, or email it to me and I can post it. jared@oneearthmushrooms.com
@@OneEarthMushrooms Thanks for the reply, Jared. I found the study over at the @Mycology Exploration channel as they also advocate an Instant Pot. The study is titled: Assessment and verification of commercially available pressure cookers for laboratory sterilization and was done @ Dakota State in 2018 A Bing search will bring it up. One of the pots, the Duo 80 I believe met their standards for lab sterilization. Thanks again for you interest here...It is great to see a knowledgeable grower exploring some of the topics that were discredited in the past. As materials and equipment change, we need to update our knowledge and teks accordingly.
There's a lot of sterilization advice that's way off. I believe the standard for medical autoclave sterilization is quick, like 20 minutes to a half an hour. Of course InstaPot is a big step down, but... if you run pressure cookers for 3-4 hours, of course your not going to have contamination problems! The point is to do this as quickly and efficiently as possible.
By far the best reply. The Journal article says, In short, 150 minutes is needed to kill off the most thermally resistant spores. Most stuff dies off much much earlier.
Hey so i know this is off topic for this video but i thought i saw a video you made making agar using peptone. I was wondering how much to use in the recipe.
Hello, is there an alternative version of polyfill (to replace it with another material) I want to use for a filter on jars for sterilization? thanks in advance
So i have an instant pot but it doesn't have the pressure cooker button or a yogurt either any suggestions? I have an instant pot IP-LUX the pre-setting options that it has is rice, multigrain, porridge, stem, slow cook, sauté, egg, cake, Meat/Stew, and Soup/Broth.
How can you tell if there is too much moisture in the sterilized grain? I tried this today, and feel like there may be too much moisture in there…thank you!
The link to the step by step didn't work for me. Here are my notes
1. Pour 1/3 Cup of Rye Grain into Mason Jar
1. OR (90 Grams of Rye grain for each pint jar)
2. Once you have the right measurement of rye for as many jars as you are going to sterilize. Remove grain from jars and place into Instantpot.
3. Add water to cover rye grain
4. Use “saute” option on instantpot to heat up grains until boil. Boil 1 minute
5. After boil, drain grains in colander, and let sit for 10-15 minutes
6. Pour grains onto sheet to and spread them evenly to allow them to dry.
1. Grain should not “look wet” should not be shiny. Looks dull, but is soft. (Took video about an hour and a half to dry)
7. Fill jars about half way full
8. Put your custom lids back onto the mason jars
9. Put tin foil ontop of mason jars to avoid extra moisture getting into the lids.
10. Put something on bottom of instantpot to avoid letting the glass jars touch the bottom of the instantpot, put few cups of water in instantpot
11. Place jars in instantpot, and select high pressure, for 1.5 hours. *disable keep warm button*
1. Guy in video did this right before bed so by the time he took the lid off everything was nice and cool.
2. Jars can be used within 1-2 days after sterilization for max results.
H, this is so helpful. Thank you!! I'll look into the link, not sure what's up with that.
Update: fixed the link! Sorry about that.
@@OneEarthMushrooms does it matter if you have some of the Rye grain burst? Thank you for your reply
3.5 - soak grains over night then proceed to 4
Could you use whole oats instead, following the same procedure?
How long can you store these cooked grain spawn jars before inocoluation?
A newbie here..
Maybe a Silly question, but is it possible to just cook and sterilize in the jars at the same time instead of pie cooking etc?
Thanks
I can’t fit my jars with the trivet, quart sized, so I don’t use it. No problem with breaking yet and I do 3hrs typically for my rye jars.
Tried this, put foil over the top and there is quite a bit of water in the jars. Should i re do it or will it be ok?
With those type of lids with just the vent patch, would you inoculate them through that vent? Thanks so much, i love the videos!
No that’s just for gas exchange you would have to open the lids
You actually could inoculate through the patch, just put a new piece of tape over the hole after inoculation. I install a rubber plug in my lids to allow for inoculation through the plug without disturbing the vent.
I bet that water you drained off would be good to moisten cvg.
What size is the instant pot?
is the lid with injection port on the cover necessary for sterilisation in the pot? or can i just use normal, completely sealing, covers? (because i have grown the mycel in agar petri dishes and plan to just open the lid and add the agar and mix.)
You still need the filter
Many people recommend venting the PC for at least ten minutes before putting the rocker on...
Would it be necessary to leave the instant pot on vent for a similar time period and then seal it, to remove all the air inside and replace it with steam like in the PC?
I think the same principle applies. I do about 5 minutes, can't hurt.
After pressure cooking and having the same jar setup as you, I always get a ton of moisture in the jar even if I completely wrap them 5 times in foil. Is that ok? After a couple of days, the grain seems to absorb some of that visible moisture in my jars.
I heard that any excess moisture that come from the pressure cooker is OK, you just shake it up and distribute the moisture throughout the other grains. I’m new to this, so I’m not sure if that’s 100% true but that’s something I have heard.
On your website the link to the written procedures for this are not to an Instant Pot version, they both link to the system old school pressure cooker write up.
Fixed it! Thanks for letting me know.
Can you standardize the moisture of the grain by weighing it after cooking it to get to a specific hydration rate? If too heavy, let it dry more? Curious what the hydration rate % should be if using this method.
I’ve seen some people do this with popcorn.
They weigh 10 cornels dry and divide the weight by 10 to get an average weight.
and then keep hydrating until they reach their desired hydration using the Same weighing method of 10 cornels and dividing
not sure which is the right number because I haven’t experimented myself.
I didn’t properly disable the keep warm function and my insta pot stayed on all night. Will the grain jars still be okay to use? Thanks for any help
I don’t see why not
Can you store fully colonized grain jar spawn in fridge, with the intention to be taken out later to inoculate substrate?
yes.
Hey, great video! Thanks for high quality education on mushroom cultivation.
Question - why do you use 4 jars? Isn't it more convenient to inoculate just one big jar? Is this because 1 won't fit into the instant pot? If you're growing the same mushroom, do you use multiple jars to have enough spawn for the 1:4 spawn -- to -- substrate ratio, if using a monotub?
Thank you!
little jars fully colonize a lot faster. and if there is a cotangent in the one Big jar you must start over. If one of the little jars is contaminated you still have 3 others.
I have been looking Everywhere for a jar larger than a Pint that has a screw on sealable lid of some sort, practically impossible. If you want it to fit specifically in an instant pot. Pint size it is.
This is true for most stovetop pressure cookers as well, although you can try putting a big jar sideways. Maybe canning pots or high end pressure cookers can take larger.
you need to sterilize the other quarts before inoculating anyway, right?
How much water do you add in with the jars before sterilizing?
Just enough to reach the bottom of the jars...about 1/2 liter.
What size instant pot do I need to take bailey jars does it have to be a 8 litre or can it be smaller?
I'm not sure what a Bailey jar is! I think your best bet is to look at the dimensions of an instant pot (available on their website I believe) and compare that against your jars.
I found I had a problem with my Instant Pot, after cooking one batch of grain jars and letting them cool awhile, when I added 4 more jars it would appear to boil but not get up to pressure~~~I found that I had to dump the leftover hot water from the first batch and re-fill it with coldwater to get it to get back up to pressure! Then everything worked fine.
There must be some kind of sensor that needs to be reset with cool water before it will allow the water to heat up to pressure temps. Has anybody else had this problem?
What litre is this instant pot?
Hi,u said that i can wait 3days before inoculation,why i can not wait longer?thanks for explanation,take care
I'm new but I believe you can actually wait longer maybe up to 7 days but the longer you wait the more likely a contaminate will grow in that substrate. Even in sterile conditions there's a chance that 0.01% contaminate might survive the sterilization method or that something got pass the sterilization method but if it had been inoculated the mushroom will help keep the substrate free of other contaminate.
When you are done with the 90 minute sterilization in the instant pot, if you are trying to speed up the cool down time, is it ok to do the pressure release at 90 minutes, or just wait until it naturally releases as it cools down?
Cool down naturally because trying to cool down fast can bring in bacteria
Definitely let it cool down slowly, every resource I've referenced says that rapid cooling increases contamination likely due to the fact that the thing cooling it (the air blowing over it, or the water) will not be sterilized itself. It is most sterile when not exposed to factors outside the pressure cooker.
@@andrewedwardsmusic if your contents are properly secured in jars/bags then blowing air wouldnt have any affect, however depressurisation can cause forced entry through gas exchange ports you might be using like micropore tape or micron strips
@@aaronpoole5655 This was it. Exactly what Aaron says. My statement was off the cuff and misleading!
but in practical terms not wrong! You don't want to find out your port isn't secured, the hard way!
So that extra 3 psi you get from standard pressure cookers isn’t necessary for mycology sterilization? Do you know if this method is enough for making agar?
liquid mediums are much easier to sterilise, so if hes making grains work with the instant pot then agar will work as well. sorry i skipped through this vid mostly but also 15psi for 30m is enough for agar. there are nice graphs on google images to show sterilisation time to PSI. 12psi requires 3h and 15psi around 80-90m. The is it necessary question really means can i get away without contam, sometimes yet but sometimes no
I successfully used this method to make agar myself. it's been 5 days and I haven't seen any contam on my plates.
A pressure caner is also about $70. And also borrowing one from a friend if you have any cool 😎 old ladies to your friends with they probably got a pressure canner.
Would the measurements be the same for whole oats?
Whole oats expands 2-3x bro an instapot is a pressure cooker idk why hes acting like its not. I always do quart jars 1 at a time. Lay it on its side and do 2H30M with oats. I use oats and an instapot 0 contams
@@saltythugproductions9686 awesome! Thanks for the info!!
Because the InstaPot company pretends it isn't a pressure cooker! and a lot of owners don't understand their InstaPot is a pc. It doesn't matter as long as one follows the right directions.😉
Make vids for no pressure cooker grow shrooms
Is this the 6 or 8 quart model?
I own the 8qt version and this one looked like a 6qt.
So I did it all last night put my pressure cooker wasn’t on will they still be hydrated enough… so stupid i am
If you could squeeze the grains to show us how much moisture is in it might be easier for us to replicate before you put it in the jars
That's amazing. I'm scared to used a pressure cooking this one seems safer and practical
to put it simply : it is!
why isnt a pressure cooker as safe a a rice cooker?
@@MrCrazy-eb5cg Because of the pressure
@@surronzak8154 huh that went over my head
@@MrCrazy-eb5cg hapens sometimes ;-)
dosnt the insta pot only get to 11 psi shouldnt you go longer then 90 minuites
In theory yes. Personally I never had problems like this. Might also be because instant pot takes a long time to build up pressure already. I think it also matters how big your substrate is. If you have one solid grow bag inside, it will probably take longer for the heat to reach the core.
@@demian2325 yes i made this comment before i tried it at 90 mins worked fine
My micropore tape keeps melting, it's still covering the hole but you can tell some of it melted off, is that still okay?
Don't use micropore. Buy an adhesive filter patch
If you figured the dry weight to wet weight ratio, you could use the exact amount of water needed in the sterilization process. Basically, eleminating the1st half of your video.
my pressure cooker is 6 litre one and cant fit the jars (unless very small). Can I use an oven to sterilize the 1 litre jars instead ?
Do you put the kids on tight ? Or leave them a little loose ?
theres a hole on top for the Micropore Tape then the needle and innoculation. Doesnt matter how tight
I put the lids on until the ring stops spinning without me adding any additional effort. I don't put it on tight, or loose....lol hope that makes sense. And I agree with @Andy Sills, it doesn't matter much.
How this is going to be a silly question is there a difference between an insta pot or Crock-Pot do the kind of work the same
No. An instapot or pressure cooker builds pressure and much higher temperature. A crock pot will never get hot enough to sterilize anything.
@@brockbaker3able thank you
Question for the educational and theoretical purposes only
Lets say you have an "All-in-one" grow bag and several 1/2 pint jars that are almost 80-90% fully colonized, but you have just been invited to go to, let us say Mexico, for 10-14 days(starting in 5 days..so around >>14 days past being fully colonized
How was Mexico? In the bag (or jar) it will be okay, even if it eats all the grain in the container. Once you take them out to fruit, presumably in a fruiting chamber, you can't leave it, at least not without harming it.
PS, this is theoretical and for educational purposes! lol
Why not wait til you get back. Then begin
@@FyourIocals theoretical the bag would have already been mostly colonized, right before the vacation started..and according to legend everything was fine, it all worked out... So Ive heard.
Can you sterilize bags in an instant pot?
Robert, I don't want to give you an answer without giving it a try. I suspect the answer is yes, but I'd like to do some experimenting. The IP is pretty small though, so I don't see it being possible to do more than one 5 lb bag at a time.
@@OneEarthMushrooms Thank you for replying.
You need a special heat resistant plastic, I've seen it called PP5, and I think it's recycling designation is also "5". Ordinary plastic, like g
Glad sandwich bags or freezer bags will melt.
Yes, im about to try this today:
ua-cam.com/video/bTeLn24rOEk/v-deo.html
@@squirlmy polypropylene is the name for it, most microwaveable food containers etc are PP
Can you put bags in an instant pot?
Yep! I can only fit one bag at a time in my IP.
@@OneEarthMushrooms how much time do you run for bags? looking to run one 3lb bag of millet and not sure about time.
Link for rye grain?
You're looking to buy the grain? I don't have any links. Depending on where you are in the world you may be able to find rye grain (not rye seed!) at your local farm store. I personally buy in bulk from Azure Standard. Takes a while to get the grain shipped, but its really inexpensive for high quality grain.
Is this the 8qt pot?
I bought some growing kits, spore prints, spawns, liquid culture and psychedelic products from an online supplier and they are affordable and discreetly delivered
FROM
TRIPWITH_JEFF
in
instagram
Link for the tape?
Search for 3M Micropore Tape. I've bought it on amazon before, but a local medical supply store would be your best bet.
Do you just drill a hole in the lids then cover with tape?
Would this method work for brown rice as well?
@don julio could I follow the videos procedures for popcorn?
The sound is not loud enough
Totally not a precise method thats not easily repeatable. Everytime you do this itll come out different. You need to figure out the exaxt amounts of grain,water, other additives so you can repeat this 100% the same everytime. Not put about 2inches of water in there. What if my pot is deeper?
Ever tried unicorn bags in these anyone?
There's controversy about Unicorn plastics no really being biodegradable, but just disintegrating into microplastics. The EU won't certify them, but they claim this is a conspiracy by European industries involved in more usual biodegradable plastic tech. IDK
Hey, I know where this is coming from. It would be kind of you if you put the references of this previously done work by other authors. Thank you.
Everyone do this tho. lol
It's coming from a ton of other growers lol
Ah man did you throw that foil away? ♻️
“This video is ad free”
Continues to advertise his Patreon.
Haha not mad or anything and I really appreciate the video! Just teasing you.
Ha! Yeah, true. I guess a more correct way to say this would be "This video is free of algorithmically targeted unskippable ads" but thats such a mouthful!