Salkowski's test: Part 2 (Identification of Cholesterol)

Principle:
Cholesterol as a derived lipid is a member of the group of steroid known as sterols. Imbalance in lipid metabolism can lead to major clinical problems such as obesity and atherosclerosis.
Method:
- 2 ml of cholesterol dissolved in chloroform,
- Add an equal volume of concentrated sulfuric acid;
- A yellow to brick-red color is formed indicating the presence of cholesterol

[@sayandutta9893]

short crisp and beautiful demo station yet again

[@Petinency]

Thank you for sharing too.

[@DrMag-fn7hc]

The chemical reaction and the reason of the appearance of the red colour is well explained in this link www.coursehero.com/file/p359l0k/Salkowski-reaction-is-the-reaction-of-cholesterol-with-concentrated-sulphuric/

[@jonBrazilian]

thank you for sharing. this example will be very useful for my upcoming biochem lab :)

[@cedricazaniharta5363]

I usually do that in acid room bacause H2SO4 is danger with High concentration.

[@cedricazaniharta5363]

But, thanks for your education 😍

[@anuragbahadur5687]

Sir Why is the red colour formed??

[@bered5946]

you can explain to me how to appear this color,pls?

[@kleinangelsedano5969]

can you explain why it turns red? Pleaseee

[@snata22]

Sulphuric acid is highly hygroscopic, it removes the -OH groups from two molecules of cholesterol and a bi-cholestadien is formed by a connection between these two cholesterol molecules, which gives the red colour.