Multiple Sequence Alignment and phylogenetic tree using Clustal Omega (Tutorial)

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  • Опубліковано 1 жов 2024
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КОМЕНТАРІ • 34

  • @abuhurairah4994
    @abuhurairah4994 2 роки тому +1

    Too much back ground noises, dear.

  • @bhushankulkarni5497
    @bhushankulkarni5497 3 роки тому +1

    What those numbers after the names of species indicates in phylogenetic tree?

  • @hasnae3492
    @hasnae3492 3 роки тому +2

    Awesome informative videos and simple explanation thank u so much im big faan of ur channel ❤❤

  • @monikasharma6426
    @monikasharma6426 3 роки тому +1

    Make a video from where you get those sequence if I want to do this for corona virus what I have to do?

  • @hibaarshad606
    @hibaarshad606 2 роки тому +1

    You have explained very well

  • @microbiologist177
    @microbiologist177 10 місяців тому

    How to save phlogistic tree

  • @poojabathini6256
    @poojabathini6256 Рік тому

    superb explanation tq so much sir for such a informative video ..

  • @amadeusantos-neto6813
    @amadeusantos-neto6813 2 роки тому

    can someone teach me how to make a phylogeny with many different sequences?

  • @MuhammadAsad-cf5vi
    @MuhammadAsad-cf5vi 3 роки тому

    Which output file has conserved region?

  • @nuranisultana2029
    @nuranisultana2029 2 роки тому

    A minimum of 2 sequence problem?? Help me sir

  • @tariqrahim1120
    @tariqrahim1120 3 роки тому +1

    Great!

  • @backup3412
    @backup3412 Рік тому

    Bhai please volume thora ziada rakhayn

  • @Joeythegoats
    @Joeythegoats 3 роки тому

    what does groups represent? in guide tree? Group 1: 2 sequences etc?

  • @matiullah6642
    @matiullah6642 3 роки тому

    How I will download the tree from Clustal Omega?

  • @mracademy1116
    @mracademy1116 Рік тому

    awaz hi nai he

  • @Bibliophile5072
    @Bibliophile5072 3 роки тому

    Is there any difference between clustal w & clustal omega

    • @FarhanHaqj
      @FarhanHaqj  3 роки тому

      Both methods have the same purpose. Omega is newer version of ClustalW that uses seeded guide trees and HMM profile-profile techniques to generate alignments between three or more sequences.

    • @Bibliophile5072
      @Bibliophile5072 3 роки тому

      @@FarhanHaqj HMM profile to profile techniques means?

  • @Joeythegoats
    @Joeythegoats 3 роки тому

    why you choose these specific sequences?

    • @FarhanHaqj
      @FarhanHaqj  3 роки тому

      It depends on your question that what you want to address

    • @Joeythegoats
      @Joeythegoats 3 роки тому

      @@FarhanHaqj i mean the sequences for multiple alignment. he told me why did u choose for example
      human
      cat
      rabbit
      fish
      snake
      etc

  • @IITMadrasRewired
    @IITMadrasRewired 3 роки тому

    Best❤️

  • @semilogosonaike5145
    @semilogosonaike5145 3 роки тому

    Thank you. Some of the base positions in the alignment do not have any scores. The score position are empty. What does that mean?

    • @FarhanHaqj
      @FarhanHaqj  3 роки тому

      Can you share the time of the video

    • @semilogosonaike5145
      @semilogosonaike5145 3 роки тому

      @@FarhanHaqj @ 4:00 In the alignment, some positions do not have any character to indicate their scores

    • @FarhanHaqj
      @FarhanHaqj  3 роки тому

      @@semilogosonaike5145 Thank you for pointing the time. As explained, the dashes "-" represent "Gaps". It means that due to some evolutionary event in Macaques, there is a deletion of these amino acids.

    • @semilogosonaike5145
      @semilogosonaike5145 3 роки тому

      Thank you for replying. I think I used the wrong terms for my question. From you explanation, “*” indicates that the alignment at an amino acid position is conserved. “:” indicates semi-conservation. But I noticed that some positions have neither “*” nor “:” nor “.”. How do we know the status of these positions when it is not indicated if they are conserved or not

    • @FarhanHaqj
      @FarhanHaqj  3 роки тому +1

      @@semilogosonaike5145 MSA tries to find the conserve region from multiple sequences. Due to the gaps (which means part of the sequence is not present in any one of the species) that part of the sequences are excluded from any abbreviation.

  • @sadafawan5254
    @sadafawan5254 3 роки тому

    Conserve region ko kia Karna ha