How To Make A Phylogenetic Tree From DNA Sequences | MEGA X Tutorial 👨🏻‍💻🧬

Step-by-step tutorial on how to make a phylogenetic tree using the MEGA X software
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#Phylogenetic_Tree
#MEGA_X
#bioinformatics
#biotechnology
#molecularbiology

[@gabriellamartino9732]

Thanks a lot! Very clear

[@MadisonMunarPhD]

thanks😊

[@wanderingfish]

Thank you for this. I learned a lot since I am a new learner in MEGA :D

[@MadisonMunarPhD]

thanks😊

[@fama4105]

Thanks for your useful information

[@MadisonMunarPhD]

Welcome😊

[@soniasoni2245]

What are the differences between all the methods maximum likelihood and minimum evolutionary method and on what basis we select these methods

[@charleschan8705]

This is the video I like best to introduce MEGA

[@MadisonMunarPhD]

thanks😊

[@ozgunteksoy4398]

thank you for this infornative video. May I request, you take a video about how this result interpretation.

[@MadisonMunarPhD]

Hi Ozgun, I have another video for the result interpretation 👉 ua-cam.com/video/QlMwSqNbKA8/v-deo.html

[@soumiasoumia4764]

Thanks for the very informative video, it was very helpful. Could you please explain to me how to construct a phylogram tree? Instead of cladogram tree on Mega

[@MadisonMunarPhD]

Hi Soussou Darine, usually we can only build a cladogram which infers evolutionary relationship among the samples being analyzed, including an outgroup can provide a sense of root or evolutionary origin which is being reflected on a phylogenetic tree, however, the tree is only an inference since the outgroup is not a definitive root of the organisms under study

[@aannajarvis4188]

Sir, While finding the best models, which one should we choose - complete, deletion, use all sites or partial deletion?

[@MadisonMunarPhD]

Hi @aannajarvis4188, you need to build three cladograms using three different building methods, then compare the clustering of the samples, if the three cladograms agree then most probably the analysis of your samples is correct and is highly supported, you can choose any of the three cladogram to use for presentation, all the best!

[@jctheexplorer1794]

Is it possible for us to show scale bar in original tree (topology only)?
Thanks

[@MadisonMunarPhD]

Hi JC, yes Neighbor-Joining and Maximum Likelihhod, usually would show a scale bar for estimation of sequence divergence, I suggest to create or test at least three (3) building methods and compare the formation of the clades, if all 3 different building methods agrees then most likely the analysis is correct.

[@jctheexplorer1794]

@@MadisonMunarPhD
Thanks for reply. I have encountered a problem that the max likelihood tree that I have constructed didn’t show the scale bar after I selected the “Topology Only” tab. Does it means that it is not possible to show a scale bar in the max likelihood tree unless we deselect the “Topology only” tab ?

[@eswinipi]

I keep getting the error that mi reads are too long, is there a limit for how long the DNA sequence has to be?

[@MadisonMunarPhD]

Hello Joanna, sometimes this error usually occurs due to the shortage of memory to store the data. You may check the available memory on your computer.

[@alzohairy]

Can you please send me the PDF file for this presentation 🙏

[@MadisonMunarPhD]

Hello, please send me email at [e-mail]

[@ag9496]

Hi. Would you know why the program may crash without any errors whilst constructing the tree? It's infuriating.

[@MadisonMunarPhD]

Hi, sometimes the program may suddenly crash due to low memory storage of the computer, or the data in the samples being analyzed is too big.

[@vinothini6168]

I got error : application error finalizing MUSCLE alignment unable to open file. please help me to rectify this.

[@MadisonMunarPhD]

Hi Vinothini, you need at least 5 sequence samples in order to perform the alignment, if this is not the case, you may try to uninstall the program and reinstall, then try to use the same sequences for alignment

[@vinothini6168]

Thank you for your reply. I have 37 sequences but got error.I will do your suggestion.