Can we apply two different lazers (e.g., FITC and PE) at the same time to excite cells, and then detect and sort only cells that are exicted by both lazers, and get data from computer? This would be the same effect by Image J. (e.g. Getting FITC and PE images, respectively from fluorescence microscope and then merge them, which generates yellow pseudo color)
Hi Range. Thank you for your question. Please contact our technical support team at thermofisher.com/askaquestion for additional information. Thank you!
![Flow cytometry basics: Overcome technical challenges [WEBINAR]](http://i.ytimg.com/vi/9DcvsIPqWWs/mqdefault.jpg)
Very informative! Thanks a thousand!
Thanks for watching.
Just done with a flow cytometry exp, very good educational resource
Hi Alex, we are glad that you found this video to be informative.
Can we apply two different lazers (e.g., FITC and PE) at the same time to excite cells, and then detect and sort only cells that are exicted by both lazers, and get data from computer?
This would be the same effect by Image J. (e.g. Getting FITC and PE images, respectively from fluorescence microscope and then merge them, which generates yellow pseudo color)
Hi Range. Thank you for your question. Please contact our technical support team at thermofisher.com/askaquestion for additional information. Thank you!
Why people would hit the dislike button!
Yes, agreed.
most likely due to the quality of the microphones.