DNA sequencing methods

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  • Опубліковано 21 бер 2015
  • DNA sequencing methods - this lecture explains Sangar sequencing method and Maxam Gilbert DNA sequencing method and next generation sequencing methods in brief.
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КОМЕНТАРІ • 221

  • @rrl9496
    @rrl9496 3 роки тому +131

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    • @shomusbiologyofficial
      @shomusbiologyofficial  3 роки тому +21

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      @asitdhir1204 3 роки тому +2

      Where are you from @Macy sir

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      @Anlykabiofficial 2 роки тому +3

      Free he is getting money from UA-cam

  • @Mettephobicemily
    @Mettephobicemily 8 років тому +134

    It's amazing how a younger guy from around the world is teaching me stuff I couldn't learn from people that are right next to me. Thank you.

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    @akshitsoni6100 3 роки тому +18

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  • @mahnoor4499
    @mahnoor4499 3 роки тому +21

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    • @shomusbiologyofficial
      @shomusbiologyofficial  3 роки тому +1

      Thank you so much for appreciating my efforts

    • @bushrazafar7522
      @bushrazafar7522 3 роки тому

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    • @Ilemaurice687
      @Ilemaurice687 2 роки тому

      @@shomusbiologyofficial hello plzz i really need that
      different methods used in the Analysis of DNA, including synthesis and sequencing

  • @sarahp.3163
    @sarahp.3163 3 роки тому +11

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  • @Timabattah
    @Timabattah 8 років тому +4

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  • @sheeanagangadoo1852
    @sheeanagangadoo1852 8 років тому +32

    Thank you, thank you, thank you... I have been looking for someone to explain to me all of the biological techniques for a long time and now finally found your channel and website - so grateful!

  • @maansisrivastava541
    @maansisrivastava541 3 роки тому +13

    I binge watch on shomus biology lectures than on any web series.

  • @SMAKA415
    @SMAKA415 8 років тому +1

    Good job. I wanted a well overview only to review general sequencing methods and this was a good video to watch. Thanks.

  • @alinasheikh7338
    @alinasheikh7338 7 років тому +4

    thank u so much for being there during exams...
    it's very much beneficial for us

  • @snatachakraborty3124
    @snatachakraborty3124 3 роки тому +1

    The 4 mins bridge amplification animation provided by Illumina was simplified into a less than a minute illustrateddemonstration by you! So clear

  • @dr.shipraagarwal2585
    @dr.shipraagarwal2585 3 роки тому +1

    HELLO, I am pursuing MDS in periodontics, and i have to present one seminar on molecular diagnostic technique, so i didnt have a single clue about anything, because of your videos now i completely understood all the techniques. your videos are outstanding. superb. I am sure in future u gonna b someting great. good luck.

  • @Ammakodumai
    @Ammakodumai 9 років тому +13

    i love allll your videos.. I am an Msc genetics and still cant understand some basic stuff but you explain very clearly. wish I could be your student..thank you soo much for this

    • @batkuparpohtam8070
      @batkuparpohtam8070 4 роки тому

      Same here im msc student still culdnnt understand biotechnology properly.

  • @dr.pranjalikirde9665
    @dr.pranjalikirde9665 5 років тому +2

    Ur just amaizng...i cnt tell hw much help m gettng frm ur vdos...thank u so much

  • @NurAlam-jd4yv
    @NurAlam-jd4yv 8 років тому +1

    Wow.......... Carry on. Million of thanks for your great work.

  • @thanawatrattanathammethee9884
    @thanawatrattanathammethee9884 8 років тому +1

    Billion of thanks guy !!You're awesome

  • @sheikhnaseer4637
    @sheikhnaseer4637 6 років тому

    one of the best and simple way of explaining........tnku sir....keep it up ...

  • @bikashdas1735
    @bikashdas1735 5 років тому +2

    you are just understood me very clearly the fundamental of the DNA sequencing. As a msc biotechnology student this is very much helpful to me. Thank you so much and heats up to you.

  • @aparajitadutta1329
    @aparajitadutta1329 6 років тому +1

    Love your lectures.. very informative!

  • @himanitrivedi1261
    @himanitrivedi1261 7 років тому +2

    thanks a lot .... i waana to just quickly revision .. .. and its very useful..
    keep it up

  • @shama_parween
    @shama_parween 3 роки тому

    if i am getting any problem in any sub or topic every topic is present on shomus biology in so easy way thank u so much 😊

  • @manojkumar-vz4kw
    @manojkumar-vz4kw 6 років тому +1

    Hello Shomu Biology. I am indeed very grateful the contents which you provide are excellent. Regarding sequencing I have a small doubt. Could you tell me exactly what is the possible role of dmso in normal pcr and also what is the role of betaine in sequencing pcr? I have been searching for this since very long time. It would be helpful for me if you can provide me with the answer. I hope my question is a valid one. Thank you in advance. Have a nice day ahead.

  • @Sunilkumar-oq7pr
    @Sunilkumar-oq7pr 8 років тому

    Dear sir you are my faqvourite...and you are god for me.....great work sir i really vry vry glad

  • @lutfunchowdhury5711
    @lutfunchowdhury5711 3 роки тому +3

    Thank you brother. It was convenient for very beginner's and too easy to run this information in my brain😇
    Love from 🇧🇩
    Keep growing, keep spreading knowledge we all wait and look forward for your videos.

    • @shomusbiologyofficial
      @shomusbiologyofficial  3 роки тому

      Glad to hear that you're getting benefit from my lectures. Please subscribe and share

  • @fatimahbrekdar6368
    @fatimahbrekdar6368 7 років тому

    thank you very much . I just want to ask ,do we consider real time seq as a NGS ?

  • @sarichowdhary
    @sarichowdhary 4 роки тому +2

    Thanks a lot. You are an amazing instructor, your learning videos really help a lot.

  • @assistantprofessor8049
    @assistantprofessor8049 7 років тому

    I like your video , its really helpful, please answer me how to add and how to select tag sequence for pcr primer

  • @sreenivaspamisetty1757
    @sreenivaspamisetty1757 3 роки тому +2

    Thank you ☺️ sir.......we are very blessed to have a wonderful teacher like you......🙏

    • @shomusbiologyofficial
      @shomusbiologyofficial  3 роки тому

      You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

  • @nourelhodaharidy2820
    @nourelhodaharidy2820 8 років тому

    Thank you. It is very helpful overview.

  • @paramveerpatil7451
    @paramveerpatil7451 6 років тому +1

    Very good way of explaining..
    opened up how I could think the ways now!! 😃😃

  • @T-Rexplorerr
    @T-Rexplorerr 7 років тому

    YOUR VIDEOS ARE ULTIMATELY HELPFUL FOR ALL. THANK YOU SO MUCH .

  • @divyavarshney6222
    @divyavarshney6222 3 роки тому +1

    Thank you sir.
    Sir which software can be used for the identify the orientation of DNA sequence....plz tell me

  • @nigeeacademy
    @nigeeacademy 2 роки тому +1

    I appreciate you really, I used your video so many times, it is very smart and understandable.

  • @sriradhabhattacharya
    @sriradhabhattacharya Рік тому

    Thank you so much for the videos. Can you please let me know whether DGGE is a classified as next generation sequencing? Thank you

  • @vidhisambhvani8276
    @vidhisambhvani8276 7 років тому +3

    oh man u r great...salute boss

  • @isissanjuan5027
    @isissanjuan5027 4 роки тому +7

    I absolutely adore the way you share your knowledge

  • @anitasingh7161
    @anitasingh7161 6 років тому

    Thank u Sir its very useful lecture for my examination

  • @thatregulargirl9388
    @thatregulargirl9388 2 роки тому +3

    Thank you Shomu bhaiya. You make studies easier and interesting at the same time.😇

  • @jaydeepdumadiya2464
    @jaydeepdumadiya2464 3 роки тому

    I'm thankful sir....
    God bless you...🙏🙏🙏🙏🙏🙏🙏🙏

  • @yashjain1296
    @yashjain1296 8 років тому

    i want to know about sanger method . btw nice video. clear voice and explanation is super. (y)

  • @_atifch
    @_atifch 4 роки тому +4

    nice sir keep it up i am impressed by you worldly knowledge.

  • @senada5959
    @senada5959 8 років тому

    A very informative video, very good

  • @sciencywiency
    @sciencywiency 5 років тому

    Thanks Shomu, your videos are super helpful:D

  • @AinaSakeenah
    @AinaSakeenah 3 роки тому +1

    Thank you. Like usual, your videos always help :)

    • @shomusbiologyofficial
      @shomusbiologyofficial  3 роки тому

      You're welcome. Glad to hear that you're getting benefit from my lectures

  • @somosreeghosh8662
    @somosreeghosh8662 6 років тому +1

    I will now be able to give my exam with ease...a big thanku..

  • @kuronotsubaki
    @kuronotsubaki 9 років тому

    Hello, very helpful video, but I have some questions. I'm a newcomer and my english is still not very good, so please, be patient.
    Method bac to bac is based on DNA replication by using bacteria plasmids and further usage of the shotgun method with sequencing. Is it right?
    Can you tell anything about IonTorrent method? Is it NGS or SMS? What id the difference between NGS and SMS?

  • @lanoanhduongthi3536
    @lanoanhduongthi3536 8 років тому

    thank you sir
    good explain

  • @Ilemaurice687
    @Ilemaurice687 2 роки тому

    Sir plz tell me different methods used in the Analysis of DNA, including synthesis and sequencing

  • @subhamoysharma6046
    @subhamoysharma6046 3 роки тому +1

    Sir you always clear my concepts ❣

  • @user-yn2vt6mi1s
    @user-yn2vt6mi1s 8 років тому +1

    great work

  • @skmasudulislam8200
    @skmasudulislam8200 6 років тому +1

    Sir please discuss on topic CODING DNA

  • @zimamalamirew4808
    @zimamalamirew4808 7 років тому

    great man, thank you to teach me heartily

  • @toppe9349
    @toppe9349 7 років тому

    Very much informative. Thank you.

  • @deryacelik7743
    @deryacelik7743 4 роки тому

    Hey it was great. But I didn't see Massive Parallel Sequencing? Cn y put this to get information pls ?

  • @sagacioussadia9679
    @sagacioussadia9679 6 років тому +1

    highly grateful to You Shomu 😃😃😃😃

  • @TruthfulPromise
    @TruthfulPromise 3 роки тому +1

    Very Clear explanation. Thank you.

  • @jothipriyanka
    @jothipriyanka 7 років тому

    Good work!

  • @Worldtravel4
    @Worldtravel4 3 роки тому

    explain the 4 different sequencing techniques in detail. and show where the primers bind for each technique can you help me

  • @alpanamishra6620
    @alpanamishra6620 6 років тому

    I had query related to parameters of megablast please help as soon as possible

  • @AileenDaw
    @AileenDaw 5 років тому +1

    Thank you! You are very didatic!

  • @shahid.baloch092
    @shahid.baloch092 5 років тому

    Thank you very much, Dear.
    Wish you good luck for your success. :)

  • @sahed87
    @sahed87 5 років тому

    Sir please make a video on glucose uptake bioassay and calcium uptake bioassay

  • @sancharidatta1014
    @sancharidatta1014 6 років тому

    Sir can u make a video about Edman degradation???if it will be possible...thn plzzzz upload it as soon as possible...

  • @viniekouamou8188
    @viniekouamou8188 7 років тому

    great video. keep it up

  • @sabaakhtar4680
    @sabaakhtar4680 5 років тому

    Kindly make a video on Helicos Sequencing

  • @gopikhisan1874
    @gopikhisan1874 6 років тому

    what is the direct and indirect methods for analysis of cloned genes?

  • @ritu5602
    @ritu5602 3 роки тому +1

    Thnkuuu sir for teaching us in this way 😍😊

  • @shirinbegum6464
    @shirinbegum6464 6 років тому +2

    Please upload, protein sequencing method, my exams are near

  • @revanth3241
    @revanth3241 Рік тому +1

    Literally saying, You are the UA-cam Radha Krishnan. Thank you for making Humans to Gain Knowledge.

  • @medicalangel2999
    @medicalangel2999 9 років тому +5

    Thankkkk uuuu u r better thn my lecturer lol

  • @Serwada237
    @Serwada237 3 місяці тому +1

    MSc molecular biology and biotechnology this is so good 🎉

  • @chefl2421
    @chefl2421 5 років тому

    Thank you sir.

  • @mayukhroy5393
    @mayukhroy5393 4 роки тому

    This was helpfull Sir

  • @avdheshsharma6346
    @avdheshsharma6346 6 років тому

    i'mma Fan i Rhyme like an insistent man you explaining Best everything you can then I'll combine the DNA Strand to build something that will be a gift to every Human Alive so that i'll increase minor bit of chances in this universe for us to Survive, Thanks for making these Videos Have a Happy Life.

  • @kiwivds1706
    @kiwivds1706 6 років тому +1

    can you please tell me where you find the accurancy and price per million bases? I would like to use it for an assignment but I need a good reference

  • @leakurtek5098
    @leakurtek5098 7 років тому

    please give the actual link to the videos you are refering to, it would be very helpful. otherwise, you rock

  • @adamprabowo4556
    @adamprabowo4556 4 роки тому +2

    Maa shaa Allah, Thanks sir

  • @manjansinghkhadiya2739
    @manjansinghkhadiya2739 6 років тому

    thank you sir

  • @obulamsnehith4423
    @obulamsnehith4423 3 роки тому +1

    Ur are just amazing keep going on

  • @garyraab9132
    @garyraab9132 4 роки тому +5

    Good overview. You must be gifted with a continuous fast talking stream gene..! Unfortunately I must have the slow hearing and processing gene! Be it true that I cannot improve my malfunctioning processing without resorting to CRISPR technology?.🙀.

  • @christinajoycy5989
    @christinajoycy5989 4 роки тому

    Is DNA sequencing method and genome sequencing both same?

  • @Doctor_Boy-01
    @Doctor_Boy-01 8 років тому

    Thank you

  • @naznintalukdar5191
    @naznintalukdar5191 3 роки тому

    What is the cooling region nucleotide sequence ?

  • @lamzaasim1252
    @lamzaasim1252 6 років тому

    I m in class12 I wanna know since all human are genetically different so how can we get a complete nucleotide sequence for a human genome .is it certain kind of average .could plz ans it early

  • @alankritarani1498
    @alankritarani1498 7 років тому

    quite helpful

  • @Aurochhunter
    @Aurochhunter 4 місяці тому +1

    I learnt this stuff years ago, it seems like ancient history now, but it's not as complicated as it seems if you actualy take the tein to learn it properly.

  • @Hemlatasingh923
    @Hemlatasingh923 9 років тому

    thank you

  • @proneyex
    @proneyex 5 років тому

    Thank you, your videos are helpful. are you in India?

  • @kavis3554
    @kavis3554 6 років тому

    very usesful this topic

  • @hanaahezam9109
    @hanaahezam9109 3 роки тому +1

    Really amazing video...thanx alot

  • @sivathesingam8945
    @sivathesingam8945 2 роки тому +1

    No Words To Say Sir...♥️♥️♥️🦋💫

  • @arsalankhan-cx6bk
    @arsalankhan-cx6bk 6 років тому +1

    Please upload the method of illumina sequencing

  • @ayeshamalik7526
    @ayeshamalik7526 2 роки тому

    Any one can please share the link of capillary method sanger sequencing

  • @ZemelakEndeshaw
    @ZemelakEndeshaw Рік тому +1

    goooooood man

  • @uzoechisamuel
    @uzoechisamuel 3 роки тому +1

    Thanks

  • @aishwaryasewatkar5280
    @aishwaryasewatkar5280 10 місяців тому +1

    Nice explanation 😊

  • @sivathesingam8945
    @sivathesingam8945 2 роки тому +1

    Thank You Sir...🙏♥️🦋💫

  • @aylinmejia4468
    @aylinmejia4468 6 років тому

    OJALA ESTUVIERA EN ESPAÑOL :(( ¿Podrían ponerle los subtitulos?

  • @samuelmwafulirwa6668
    @samuelmwafulirwa6668 8 років тому

    thanks

  • @Nittya555
    @Nittya555 10 місяців тому

    Sir can u bring us tha proper notes regarding this topic. Plzz