How to Calculate Protein Concentration of Unknown Sample from Standard Curve in Excel

In this video, we demonstrate step-by-step how to calculate the protein concentration of unknown samples using a standard curve prepared using absorbance and concentration in Excel. We use the BCA protein assay as an example to illustrate the process. By the end of this video, you will have a clear understanding of how to calculate protein concentration using a standard curve in Excel, which is a fundamental technique in protein biochemistry such as western blot and other applications.
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This video is for educational purposes only. Copyright Disclaimer Under Section 107 of the Copyright Act 1976, allowance is made for "fair use" for purposes such as criticism, comment, news reporting, teaching, scholarship, and research. Fair use is a use permitted by copyright statute that might otherwise be infringing. Non-profit, educational, or personal use tips the balance in favor of fair use.
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[@paporisharma2936]

Such simple explanation, great!

[@BiologyLectures]

Thank you

[@virgirma1328_PhD_scholar]

Thank you so much sir for the explanation !It s the 1st video I relate to!

[@BiologyLectures]

Thank you very much for the kind words. 😊

[@virgirma1328_PhD_scholar]

Can you please make a video on the amount of proteins needed to be loaded on SDS for WB?

[@BiologyLectures]

You can load 10 ug to 50 ug of the protein. Im fact the amount of protein you load depends on multiple factors. 1. The basal expression level of protein .
2. The amount of primary and secondary antibody i.e. the dilution of the antibody used.

[@dikshyapanthi7681]

Very good explanation 😮

[@BiologyLectures]

Thank you 😊

[@zelalembanjaw9687]

Thanks.

[@BiologyLectures]

You are most welcome 🤗

[@fatimaaldali577]

thank you nice lecture

[@BiologyLectures]

You are most welcome

[@khanshab5173]

Previous vedio plz

[@easybiologynotes]

Very good 😊

[@BiologyLectures]

Thanks 🙏

[@joannadevung4358]

How do i calculate the enzyme activity in order to get the specific activity?

[@muhammadsulaiman6023]

today i was doing the same and suddenly i find you

[@BiologyLectures]

Glad it helped

[@mybooks3160]

My internet is in negative -0.3.... And slop is 0.2 and R2 is 96.1
But this is not giving proper concentration