Detecting Proteins at 280 nm by UV-Vis: Why? How?

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[@Lejonet120]

Thanks for that, keep ON..

[@amjadm6772]

I'm confused about one thing. How can we come up with the calibration curve to begin with if we also don't have a proper way to measure the concentration of the samples??

[@minenurayten2134]

great video and explanation!! thank you

[@RohitPant04]

Nice job explaining the entire process along with the concept. Thanks!

[@ZunairaFatimaWUM-BCH--]

Sir..plz tell me... the protein that is placed in spectrophotometer for measuring it's absorbance is with diluted ur diluted???....mean first of all protein from stock solution is taken and then diluted with solvent and then placed in spectrophotometer for measuring it's absorbance????..

[@eunicetan5960]

hi what contributes to a more delocalised electron cloud such that it has a greater absorbance,why wont phenylalanine have greater absorbance since lone pair from oxygen can delocalise into the benzene ring ?

[@vasunakili5611]

Can we detected corona kind of protein

[@ioannaprasopoulou9016]

Thank you very much for your video :)

[@CatalystUniversity]

No problem!

[@kleineblumenvongarten5210]

Perfect!