MS Analysis of MHC Class I- and II Peptides : Immunopeptidomics | Protocol Preview

Watch the Full Video at www.jove.com/v....
Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis - a 2 minute Preview of the Experimental Protocol
Isabelle Sirois, Maxim Isabelle, Jérôme D. Duquette, Frederic Saab, Etienne Caron
CHU Sainte-Justine Research Center,; Université de Montréal, Department of Pathology and Cellular Biology, Faculty of Medicine;
Here, we present a protocol for the purification of MHC class I and class II peptide complexes from mouse and human cell lines providing high-quality immunopeptidomics data. The protocol focuses on sample preparation using commercially available antibodies.
Visit www.jove.com? to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: / jovejournalofvisualize...