Fuchou Tang - Single Cell Omics Sequencing Technologies: The Third Generation

Professor Tang introduced various single-cell long-read sequencing technologies developed by his team. To investigate the mechanisms by which multiple genomic elements were involved in the regulation of gene expression through three-dimensional genomic conformational changes, Tang's team used the single molecule sequencing platform to develop a single-cell long-read concatemer sequencing method to reveal high-order chromatin structures within individual cells, scNanoHi-C. The algorithm was optimized according to the unique features of scNanoHi-C data, which can identify enhancer-promoter co-regulatory regions in the whole genome. Professor Tang then introduced the scNanoCOOL-seq technology, which was developed by combining the third-generation sequencing platform and the principle of scCOOL-seq. The technology enabled the simultaneous and precise detection of information at multiple omics levels such as the genome (CNVs), DNA methylome, chromatin accessibility, and transcriptome in a single cell. Using this method, they systematically mapped the dynamics of the epigenome in mouse blastocyst stage embryos at single-cell resolution in multiple omics levels.