Nice explanation in general, however, coding and non-coding strand are labelled incorrectly (check en.wikipedia.org/wiki/Coding_strand). Also I wouldn't use the say "Gene", since the DNA plasmid only contains a very short sequence of the gene to be knocked-down. And I wouldn't label the transcript as mRNA, since the plasmid DNA is transcribed into (pri)shRNA and does not have the typical mRNA features (5'cap, UTRs, CDS, Poly-A tail). Hope this helps others who may be confused.
Hi, I wonder if Non-coding strand ( 8:50 ) can code some other genes or it's always the non-coding region when it is opposite the coding strand... in this case gene A ?
Nice explanation in general, however, coding and non-coding strand are labelled incorrectly (check en.wikipedia.org/wiki/Coding_strand). Also I wouldn't use the say "Gene", since the DNA plasmid only contains a very short sequence of the gene to be knocked-down. And I wouldn't label the transcript as mRNA, since the plasmid DNA is transcribed into (pri)shRNA and does not have the typical mRNA features (5'cap, UTRs, CDS, Poly-A tail). Hope this helps others who may be confused.
That's really a great series of shRNA introduction! Thanks man!
Very helpful. Could you possibly make a Cre-lox video?
Nice lecture! Thank you very much :) Maybe make a CRISPR CAS vid too? ;)
Very clear!!! tks!
Hi, I wonder if Non-coding strand ( 8:50 ) can code some other genes or it's always the non-coding region when it is opposite the coding strand... in this case gene A ?
lovely acent!
great stuff, realy helpfull. perhaps speed up a little bit, i.e. if any wants to learn about shRNA ko they should know basic DNA structure!
Great stuff, very clear and comprehensive. Thanks :)
Sorry I got lost, the pri-shARN is in the plasmid or how?
Tip: Increase the playback speed 1.5x or 1.75x
non coding stand acts as a template for mRNA synthesis.
I think you made mistake of coding and non coding strand