Hi sir, I have been following your videos since long. Awesome just awesome.... Now, I have a query, perhaps only you could answer me. Because even our R& D couldn't answer this. We are using c18 prontosil column for hair color ingredients quantification. Very complex compound are there in the sample (P - phenylene diamine, m-amino phenol, resorcinol, Hydroxylamine ethanol diamine and diamonio phenoxy ethanol). Issue are : 1.very low injection run (hardly 150) 2. Peaks broadening 3. Tailing 4. High pressure drops Please give a proper solution where we could make an efficient use.
Sir 342 ppm ka hard water bnane k liye calcium chloride and magnesium chloride lete but y kese pata chlta h ki itna lena h sir plz tell the calculation
Sir ye btao aap ki 5 injection 2 percent hi kyo aur 6 injection me rsd 3 percent hi kyo same result dekhne k liye 2 or 3 injection se bhi dekh sakte to hume itne injection lgane ki kya need hai
this may be due to hplc not working properly and possible caise are air bubble in line purging not done as per programming RT variation due to temp. variation
Sir apne ekdum SYSTAMATIC WAY me samjhaya ..thank u sir..all doubt clear
Mere aaj tak ke sare daughts clear krrrr diye sir aap ne thankuu sooo much such a nyc explanation 😍
So good I like this video for learning about resolution power and retention time 🙏🙏
Superb explanation. All point clear
Sir make a video of Differnce between RT and RRT
Thanks my heart touching Sir please always load HPLC related topics .
Sir,your teaching system I like very much.Thanks Sir.
Thanks for this video, excellent explanation
Hi sir, I have been following your videos since long. Awesome just awesome....
Now, I have a query, perhaps only you could answer me. Because even our R& D couldn't answer this.
We are using c18 prontosil column for hair color ingredients quantification. Very complex compound are there in the sample (P - phenylene diamine, m-amino phenol, resorcinol, Hydroxylamine ethanol diamine and diamonio phenoxy ethanol).
Issue are :
1.very low injection run (hardly 150)
2. Peaks broadening
3. Tailing
4. High pressure drops
Please give a proper solution where we could make an efficient use.
Tq so much sir,🤝 iam from hydrabad.
Very Helpful 👍👍👍
Sir gradient method kaise banate hi please sir is per video bana digiyega.
Great video sir
Good effort and teaching way
Sir I think plates limit is NLT 2000 and tailing is in between 0.8 to 2
Sir 342 ppm ka hard water bnane k liye calcium chloride and magnesium chloride lete but y kese pata chlta h ki itna lena h sir plz tell the calculation
Sir gas chomatograpy per vedio banaye
Thank you sir for systmetic discussion
Sir ye btao aap ki 5 injection 2 percent hi kyo aur 6 injection me rsd 3 percent hi kyo same result dekhne k liye 2 or 3 injection se bhi dekh sakte to hume itne injection lgane ki kya need hai
Hplc washing kese Kare is topic per video
How to wash colum first washing hplc water, methanol etc??
Sir Chromeleon 7 ka operating system ka full video banaye
Great 👍
Sir Assay. Purity an Potency pr ek video upload kare please
Sir which guide line tells about system suitability parameters?
Sir kindly impurities calculation py video bnay
sir,
tailing can also happen when sample concentration is high
Thanks sir
sir, can u explain dead volume, void volume, dwell volume and carry over
Mobile phase par video banye
Sir if 5 sample are injected one by one and area of each sample is greater than the previous one whats the reason
Sir agar mobile phase ka PH hi nhi kra ho to
Tailing should be less than 2..but what is the unit of 2 ?
Unitless
Nice sir
Thank you sit
Apne Kon si university se clear ki hai graduation sir ??
Cefixime me aksar resolutions fail hojata hai sir
👌
Sir HPLC gc training fees kya hai, please give me information
Sir please information column chemistry
Sir suppose three standard area gets constance but Last two standard aeras different what reason for this types of issues
this may be due to hplc not working properly and possible caise are air bubble in line
purging not done as per programming
RT variation due to temp. variation
some more causes are there as per running condition of HPLC
Mahesh kale ,Aniket gavade
What about capacity factor. (K), Bracketing, and RT rsd
Sir aap kaha se he
How much PH Mobile Phase
Sir,
Good morning
Sir RRT kya hota h in HPLC
RRT is relative retention time of peaks with compare to main peak RT
Agar peck break ho Jay to
Sir reference batado inki☺️
Sir, how can we increase resolution between peaks other than changing MP ratio (gradient method).
Shimadzu service engineer watching
Aniket gavade
Plz speking english sir
Tailing concept wrong
Great job sir
Thanks sir
Thank you sir.
Thanks sir
Thanks sir