HPLC TROUBLE SHOOTING I PART-2 I HINDI

[@shubhamwellnesspoint649]

thank you so much sir mujhe ye cheeze pta tha but inko troubles shooting kehte h ye nhi pta tha me jb v koi v analysis krta hu insb cheezo ka dhyan rakhta h it's very helpful for whole analysis thank u

[@tanweerhussain5554]

Sir hr column ki washing same Hogi? Jesy sugars detector column hai , us ko be aisy hi wash kren?

[@dudhatradushyant4986]

during analysis can i add mobile phase if volume is lower ??

[@prof.karanajaygupta]

YES BUT ADD CAREFULLY AVOID AIR BUBBLES.

[@dudhatradushyant4986]

Ok sir thank you 😊

[@sudarshanyellewad9521]

You can add mobile phase if it is original... means it is from the same stock of mobile phase, otherwise if you prepare new one, RT shifting may happen

[@shubhamwellnesspoint649]

yes added m.p. when your system is alter and then added blank and continue

[@rashmibhati333]

Hello sir, can u tell me why pH is 14 not 15 or 16..please sir

[@himanshumishra984]

Water disociation constant is 14

[@roshanughade3876]

Dear sir,
How to wash column in right direction or back direction...

[@shubhamwellnesspoint649]

its depend on injection

[@PriyankaKumari-xj3xw]

Always use right direction for column safety.

[@Manojkumar-hm8wg]

why uses regent in mobile phase what role of pain mobile phase

[@prof.karanajaygupta]

plz watch my HPLC series.

[@raheeljafiry4086]

Very informative video 👍,sir can you guide me about peak fronting,what are main causes of fronting and how we solve this

[@PriyankaKumari-xj3xw]

Sir g .....agar ek column n ek hplc me 3 product ek hi bar run karna possible hai ki nhi ?????

[@sachinverma9578]

RS test me main peak se pehle or baad me kuch choti choti peak aati hai kyo?

[@parthgajjar532]

why we do hplc calibration ?. always start the sequence 1st sst run then found the system are ok & not . then find are system is ok but why we do calibration ?

[@prahladmaddheshiya5973]

Sir kis condition me isocretic and gradient mode use karte hai

[@prof.karanajaygupta]

Please watch my HPLC series.

[@siddharthgarud2983]

@@prof.karanajaygupta hello want to know about HRMS

[@prahladmaddheshiya5973]

Please define complete trouble shooting in HPLC as RT shift and buffer solution as so

[@saimaumer2241]

Can we minimize telling factor of any column ? plz tell sir

[@prof.karanajaygupta]

NO ,ONLY SPECIFIC COLUMN .

[@PriyankaKumari-xj3xw]

Sir g agar harek product k liye individual column use kare company toh mujhe lgtaa hai ki kitne kitne thak jayegaaa...... practically yaisa nhi hai

[@md.morshedurrahman5940]

Dear Professor , Thank you so much for your valuable information.

[@amitkhajuria01]

Mobile phase kaise calculate kre..kitna lge ga

[@shubhamwellnesspoint649]

flow rate × run time × totle injection=

[@shubhamwellnesspoint649]

Wonderful sir , thanks

[@kamendragangwar574]

Sir hplc par new drug ke liye method development kese karte hai

[@a.n21-16]

Thank you very much for informative video. Can you please tell is it possible different mobile phase cause shifting of peaks of same drug ( mobile phase 0.1% TFA and 1% AA they are causing shifting of Rt of PGA polymer)

[@nileshmahajan2754]

Why use gradient method in hplc

[@alimir8112]

Informative video Dr sab.

[@mehwishakhter8405]

Very informative sir, Thank you so much 😇

[@ranjeet.komal.katiyar1634]

Hello kaun se group me job krti ho

[@mohd.tosifkhan652]

Very good content and delivery in easy language. 👍

[@pravinsingh8170]

Sir online class hota hai

[@ranjeet.komal.katiyar1634]

Ryt Sir thanks for knowledge dene ke liye

[@wildlifewithsawan]

Hye sir RSD fail hone ke aur bhi reason ho sakte kya?

[@shubhamwellnesspoint649]

improper filling of vails and contamination, system not properly flushed and column not saturated

[@mohammedjavid395]

Sir please make video on caffeine analysis of beverage.

[@sachinvete365]

Nice video sir. Please make video on GC trouble shooting

[@chemophilic6258]

nice explanation , very helpfull video

[@lalit5415]

Sir void volume or bad volume kya hota hai kaise calculate karte hai please eske liye bhi ek video bnao

[@bhojpratapsisodia8909]

A\

[@AbhijeetAjanikar]

Very nicely explained sir...👍👍👍👍

[@jagdishparmar8170]

Please solution for HPLC high pressure coming .

[@nadeemiqbal4742]

Thank you sir.

[@prof.karanajaygupta]

welcome.

[@surajbandrao3151]

Sir methanol se last me cleaning hogi to silica dry rahegi na

[@ajkhatri3646]

Pharma me water methenol 8:2 hota hai

[@Sultanmalik726]

Sir please iso 17025 ke topic par vedio banaiye bahut popular hogi max analysts ki ye problem h

[@prof.karanajaygupta]

thanks i will make .

[@vishakhalad4815]

Hii Sir, Myself vishakha
What precautions to be taken while using gradient mobile phase to have proper baseline

[@prof.karanajaygupta]

Filter mobile phase and saturate column by mobile phase for 1 hour .

[@vishakhalad4815]

@@prof.karanajaygupta is there any limit for drift in baseline? Acceptance criteria?

[@prof.karanajaygupta]

No but if seen drift and peak is not acceptable please change column.

[@AbhijeetAjanikar]

How to remove Carry over peaks, extra peaks, solve high baseline and get stable baseline????

[@shubhamwellnesspoint649]

insert blank and when u start tha analysis saturated the column with m.p. atlest 30 min

[@sanjeevkumardubey2290]

Nice information.. Sir

[@prof.karanajaygupta]

welcome.

[@meghagosain1284]

Please ellaborate isocratic and gradient point.

[@ravindrapandey2164]

Thank you sir

[@prof.karanajaygupta]

Welcome

[@ganeshkapse4107]

Sir, incidents, deviation,CAPA - defination and no properly information but , very important

[@SONUkumar-li6po]

Thank3

[@shraddhagupta9577]

Air bubble come in my HPLC system
how to remove bubble ..?

[@Manojkumar-hm8wg]

what dependent of ph in mobile phase

[@prof.karanajaygupta]

very important.

[@rajeshpawar4651]

PH is very important for ionising compound, Acidic compound is more retained in acidic PH, Basic compound is more retained in basic PH

[@ketanpadvi7068]

Good information

[@prof.karanajaygupta]

thanks.

[@bablukumarshah8323]

Thank you Sir grateful information

[@ijazahmad8226]

Can u plz tell us hplc calibration.. Really need of this

[@prof.karanajaygupta]

Video will release on April May.

[@pravinsingh8170]

isi 3 video hplc banaiye aur usme clear kar dijiye mai apka sara vido dhekta hu

[@prof.karanajaygupta]

thanks for suggestion.

[@pravinsingh8170]

sir pick hota kya hai pick kya batata hai

[@prof.karanajaygupta]

pick mean graph.

[@AnitaSharma-fy8fo]

RT variation acceptable range Kitna Hona chahiye

[@bhausahebhingade3541]

RT Ka percent RSD 1 percent

[@EnglishExcel2208]

Thanks a lot sir
#EnglishExcel

[@surajdwivedi6694]

sir please explain negative peak

[@nanotechnology107]

Sir can we study biological molecules by GC MS???

[@Rahulmore2010]

Thanks sir, nicely explained 👌

[@theabilstatus6652]

Mobile Phase Filter karna kitna imp hai...?

[@prof.karanajaygupta]

very important it directly relate to your column.

[@sanjeevkumardubey2290]

Sir can you tell me how to calculate retention factor, theortical plate, resolution and tailing factor.

[@prof.karanajaygupta]

It is system generated no need to CALCULATE.

[@sanjeevkumardubey2290]

Ok.. Sir
Any formula for this?

[@AnitaSharma-fy8fo]

Retention factor, theoretical plate, resolution, tailing factor is system generated.but definitely there will be formula.then only we are providing formula to the system.what is that formula

[@PawanKumar-le4pv]

Good sir

[@r.k.studio4608]

Thanks sir

[@sanjayrajput210]

tnx sir ji

[@gauravraut4833]

Sir Empower Software Method and Integration Operation video please!!!🙏🙏

[@prof.karanajaygupta]

I already made video on that plz check.

[@gauravraut4833]

@@prof.karanajaygupta Ok thank u sir...i will check👍

[@hitendradabhi3687]

Wash column with water and acetonitrile (30:70) for best column life

[@santoshkhilari2090]

Very imp point sir, thanks sir

[@bikramkumarlenka4948]

tq

[@amitsinghchauhan7923]

Thank u sir😊

[@amitkhajuria01]

Sir apki teaching me romance hai..

[@Dusk-Dawn16]

How to resolve pressure fluctuations in hplc ?

[@Harikrishna-ey6jr]

By doing purging (twice) with 10ml

[@sudanesegirl1]

Please activate English subtitle

[@Prashantkumar-ve2iv]

Thank u so amuch

[@shwetachauhan9149]

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[@nadeemiqbal4742]

Thank you sir.

[@prof.karanajaygupta]

Most welcome

[@sagaradlinge9104]

Thank you sir

[@prof.karanajaygupta]

Welcome

[@perumallachinna568]

Good sir

[@ankitbhardwaj1910]

Thank you sir ❤️

[@monlina303]

Thank You Sir