A new and improved 'gold standard' for complete plasmid verification

During this Knowledge Exchange, three presenters from Oxford Nanopore Technologies shared a comprehensive overview of the workflow for rapid, cost-effective sequencing of complete plasmid constructs.
Viewers learnt how to implement this end-to-end workflow to characterise the sequence of entire plasmid constructs rapidly, locally and at a competitive price. This enabled several advantages over other methods. For example, users could:
• Easily verify if the plasmid insert and backbone are free of mutations
• Completely avoid the need for primers and complicated primer walking
• Resolve repetitive regions, dimers and deletions
• Work across range of plasmid/BAC sizes and complexities
• Implement a simple end-to-end workflow with rapid turnaround time
• Keep the process in-house or at a local institution, ensuring data remains secure