rtPCR animation
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- Опубліковано 16 вер 2024
- RT PCR animation - This lecture explains about the RT PCR also known as the real time PCR. Realtime PCR is a technique of amplifying DNA fragments with polymerase chain reaction and along with the PCR. it can help us to monitor the concentration of amplified DNA Replication in real time with the help of fluorescence emission. Real time PCR or RT PCR uses fluorescence resonance energy transfer or Fret to detect the fluorescence generated from the DNA amplification.
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Thank you for watching the tutorial on RT PCR animation.
I think you've mixed up the nomenclature a bit. The video is about is qrtPCR (or qPCR) - quantitative real time polymerase chain reaction. RT-PCR stands for reverse transcription PCR, which is a different method from qrt-PCR, involving no fluorescent dyes as RNA is electrophoresed and Southern blotted :)
Good catch! I am just recently learning about these types of laboratory techniques and in one of my classes I was thought that after the RNA is elecrophioresed we use a Northern blot. Would you be willing to clarify this for me. Do we use a Southern or Northern?
For RNA analysis
Mignot Aklilu Northern for a RNA pool.
Southern blot is for DNA. Please make a correction before people get the misinfo
Ha ha!
Dis is Quantitative real time PCR .....Thank you sir.....
+Avini deity you're welcome
Heading is rt- PCR. rt means reverse transcriptase mediated PCR. But the matter is based on Real Time PCR or qPCR
I'm a med student and rt-PCR it's a pretty used acronym which stands also for Real-Time PCR or qPCR too. I think there is big scientific misunderstanding.
True ... I always get confused 🥲
Awesome 👌👌👌👌4 min..Mai concept clear
You're welcome
Thank you so much
This was very helpful
You're welcome
great job
rtPCR is different from Real Time PCR
THIS LECTURE is explained about REAL TIME PCR. not reverse transcription PCR
두뚜르 but his title is RT pcr which is short for reverse transcription
Thanks alot, can you please exlplain more about how to read the PCR result from the curve and why sometimes results not clear and sample repeated
so it seems like your quantitation is dependent on having excess amounts of probe. How do you account for nonspecific DNA binding due to having excess probe?
You can easily circumvent that issue by simply having a more specific probe.
Many thanks for yet another amazing educative content. Helped in obtaining a deeper understanding of the topic.
Could you please explain what "background level" means?
Thanks in advance!!
Awesome
thanks
Hi! You rock but your title is still misleading 2 years from now. It's qRT, not RT PCR you're talking off!
Please change the title. This is how students get so damn confused.
Sir,
In real-time PCR, Why Extension occurs just after annealing?
Why the primers/probe are added first not the dnTP's. What makes dna polymerase not working then?
Does Dna Polymerase always need s a primer(sequence) to start it's job?
Yes, it can't start by itself unless there is already a sequence(in this case a primer) where it can start
I have read that flourescence is shown when the probe paired with the dna molecule.....but acc to u it shows after the degradation of probe?
No. In this type of probe, after degradation
Plz make a video in Anchored PCR
Sure
why we use random hexamer primers????
Hello,
what dyes have this quencher and reporter probe together? I know SYBR green doesn't.. or does it?
A kinda late answer, but you are correct, SYBR green directly bind between the generated DNA-strands. An example of a probe using reporter+quencher- combinations would be the taqman probe.
@@09csr never late, thank you!
Does the probe only ever bind to one strand of the target DNA?
Well, it will bind to the sequence it is primed to bind to. So if the same sequence can be found on either strand in the same direction, it could bind there, leading to false positives. This is why qPCRs need to balance probe specificity properly: a too "vague" probe can lead to unspecific bindings, whereas a too specific probe might risk yielding low-value results.
This is Real Time PCR not RT PCR
Both are equal. 🤓
@@Shubhampatil-wp3gc no real time PCR is qPCR (quantitative real time PCR) or rtPCR is reverse transcriptase PCR
Why don't you change the name of your video? It's misleading
Isn't it a bit too complex?
This is not RT-PCR. You are teaching quantitative-PCR (qPCR).
Kindly change the name of the technique.
Real-time pcr can be called as rt pcr too. Proper way to call it will be quantitative real-time pcr
Worst