Hi Indu! TE buffer is also known as T10E1 buffer due to the working concentration of Tris and EDTA. Therefore, 10X TE buffer is 0.1M Tris HCl and 0.01M EDTA. Tris or Trizma® base is alkaline and stable with or without HCl. However, Tris or Trizma® base alone is much more alkaline, pH 10 - 12. Whereas Tris-HCl has a buffering capacity of pH 7 - 9 which is much closer to the target for the TE buffer we prepared at pH 8. EDTA also has a pH of 8 and this is where DNA & RNA are stable. Please let us know if you have any other questions!
Hi Indu - The two components serve distinct purposes in the buffer and therefore have different molarity requirements. TE buffer’s alternate name, T10E1 Buffer, signifies the concentration ratio of its two components, Tris and EDTA, which are present at 0.1M and 0.01M, respectively. Tris serves to maintain the buffer's capacity, while EDTA acts as a chelating agent, effectively binding metal ions and rendering them unavailable for degrading the DNA/RNA. Let us know if you have any other questions!
The 10X TE stock can be stored up to 3 months at room temperature, but can also be refrigerated and used as needed to prepare 1X TE working solution. Please let us know if you have any other questions!
Thanks for your comment! To prepare 0.1M Tris-HCl, remember, 0.1M is the same as 0.1 mol/L. We are making 0.5L and will need the molecular weight (MW) for Tris-HCl: 157.594 g/mol. Let’s calculate: 0.1 mol/L x 0.5L = 0.05 mol. Next, multiply by MW: 0.05 mol x 157.594 g/mol = 7.88 g Tris-HCl Please let us know if you have any other questions! Happy to help.
Thank you for your question! Yes, it can be autoclaved or sterile filtered if desired, depending on your application and standard lab practices. After autoclaving, remember to let it cool and check the pH to ensure it hasn't changed.
It's not recommended to resuspend DNA in 10X as it's too concentrated with salt. Instead, dilute the buffer stock 1:10 for a 1X TE working solution for resuspension or dilution of purified DNA or RNA. For storage, you should freeze it at -20 to -70C, but avoid too many freeze thaws. One tip is to make several aliquots for your different assays. Please let us know if you have any other questions!
In TE,tris base is used or tris hcl; i am confused. And why the molarities of tris and edta is different. Please explain.
Hi Indu! TE buffer is also known as T10E1 buffer due to the working concentration of Tris and EDTA. Therefore, 10X TE buffer is 0.1M Tris HCl and 0.01M EDTA. Tris or Trizma® base is alkaline and stable with or without HCl. However, Tris or Trizma® base alone is much more alkaline, pH 10 - 12. Whereas Tris-HCl has a buffering capacity of pH 7 - 9 which is much closer to the target for the TE buffer we prepared at pH 8. EDTA also has a pH of 8 and this is where DNA & RNA are stable.
Please let us know if you have any other questions!
@@MerckLifeScience thanks for the reply."but why these two should be in different molarities?"
Hi Indu - The two components serve distinct purposes in the buffer and therefore have different molarity requirements.
TE buffer’s alternate name, T10E1 Buffer, signifies the concentration ratio of its two components, Tris and EDTA, which are present at 0.1M and 0.01M, respectively. Tris serves to maintain the buffer's capacity, while EDTA acts as a chelating agent, effectively binding metal ions and rendering them unavailable for degrading the DNA/RNA.
Let us know if you have any other questions!
For how many days does 10X TE can be stored?
The 10X TE stock can be stored up to 3 months at room temperature, but can also be refrigerated and used as needed to prepare 1X TE working solution. Please let us know if you have any other questions!
How you calculate tris hcl
Thanks for your comment! To prepare 0.1M Tris-HCl, remember, 0.1M is the same as 0.1 mol/L. We are making 0.5L and will need the molecular weight (MW) for Tris-HCl: 157.594 g/mol.
Let’s calculate: 0.1 mol/L x 0.5L = 0.05 mol. Next, multiply by MW: 0.05 mol x 157.594 g/mol = 7.88 g Tris-HCl
Please let us know if you have any other questions! Happy to help.
Autoclave it?
Thank you for your question! Yes, it can be autoclaved or sterile filtered if desired, depending on your application and standard lab practices. After autoclaving, remember to let it cool and check the pH to ensure it hasn't changed.
To store dna should I use 10x or 1x TE buffer
It's not recommended to resuspend DNA in 10X as it's too concentrated with salt. Instead, dilute the buffer stock 1:10 for a 1X TE working solution for resuspension or dilution of purified DNA or RNA. For storage, you should freeze it at -20 to -70C, but avoid too many freeze thaws. One tip is to make several aliquots for your different assays. Please let us know if you have any other questions!