How do you separate the thiazoline derivative from the peptide ? Or do you add the aqueous acid in the same tube as the peptide and thiazoline derivative?
In protein sequencing, proteins are typically sequenced in fragments rather than as a whole. This approach is necessary due to the complexity and length of proteins, which can contain hundreds or even thousands of amino acids.
For example, in case of Edman's Degradation Method, when Phenylthiohydantoin - Amino Acid derivatives are formed, we perform another technique such as Chromatography or Electrophoresis and then we could know that which amino acid it is. One by one, we can identify the whole sequence of polypeptide (Sequence means what amino acid is present one after another). Hope you find it helpful!
From the sequence of codons in the nucleotide m-RNA. The starting codon is 5'-AUG which codes for methionine. Thus, to know the amino acid sequence in a polypeptide which is expressed by a nucleotide, you need to know the base sequence of the nucleotide. However, there are other issues like coding strand/non-coding strand if the nucleotide isolated is a DNA.
Sir your explanation is tooo good.. i m watching this video for my subject bioinformatics can you please make a lecture on bioinformatic tools?.for protein sequence analysis.my exam is on friday…plz…
As of now, I cannot do lectures on bioinformatic tools due to time constraints. But, in future, I will definitely make videos on bioinformatics tools. And, thanks for your kind words!
You are right. I have included only one important method here. The alkylated-thiohydantoin (ATH) method for C-terminal sequence analysis exploits some unique properties of the thiohydantoin (TH) ring system. The ATH method incorporates alkylation of the C-terminal TH with an aralkylbromide reagent.
I would love to watch this video. Unfortunately I can't follow up because of the language. I think It would even be better if it was well done in English through out without mixing of languages.
Sir your lectures are really good... Thank you so much sir...
Best Explanation 🙏
Thank you sir 🙏
Amazing sir g
I did not understand the language but I understood almost all the concepts. Great explanation, thank you!
I will be making videos in English. Keep learning! 😊
Thank u Sir
How do you separate the thiazoline derivative from the peptide ? Or do you add the aqueous acid in the same tube as the peptide and thiazoline derivative?
Your lectures are really so understanding and amazing... ❤thank you so much sir please keep it up...
Thank you for the knowledge
Appreciated sir👏
Please teach in English for some of us who don't understand indian language too since your videos are good
As I received lots of suggestions, my next videos will be in English.
Ye complete protein sequences hai kya 🤔
In protein sequencing, proteins are typically sequenced in fragments rather than as a whole. This approach is necessary due to the complexity and length of proteins, which can contain hundreds or even thousands of amino acids.
Sir how to get polypeptide sequence from extracted nucleotide?
For example, in case of Edman's Degradation Method, when Phenylthiohydantoin - Amino Acid derivatives are formed, we perform another technique such as Chromatography or Electrophoresis and then we could know that which amino acid it is. One by one, we can identify the whole sequence of polypeptide (Sequence means what amino acid is present one after another). Hope you find it helpful!
From the sequence of codons in the nucleotide m-RNA. The starting codon is 5'-AUG which codes for methionine. Thus, to know the amino acid sequence in a polypeptide which is expressed by a nucleotide, you need to know the base sequence of the nucleotide. However, there are other issues like coding strand/non-coding strand if the nucleotide isolated is a DNA.
Sir your explanation is tooo good.. i m watching this video for my subject bioinformatics can you please make a lecture on bioinformatic tools?.for protein sequence analysis.my exam is on friday…plz…
As of now, I cannot do lectures on bioinformatic tools due to time constraints. But, in future, I will definitely make videos on bioinformatics tools. And, thanks for your kind words!
Thnkq so much sir😍
Sir there is one more method for c terminal i.e. Thiohydantoin method..
You are right. I have included only one important method here.
The alkylated-thiohydantoin (ATH) method for C-terminal sequence analysis exploits some unique properties of the thiohydantoin (TH) ring system. The ATH method incorporates alkylation of the C-terminal TH with an aralkylbromide reagent.
Very informative bundles of thanks for yuh
Ong loved this explanation, thsnkss!
I would love to watch this video. Unfortunately I can't follow up because of the language. I think It would even be better if it was well done in English through out without mixing of languages.
Would make videos in English in future. Thanks for writing
Book reference bataiye sir 🙏
Reference book name bataye sir
You can refer to the Pathfinder life sciences.
@@BioXLDCould you please mention the unit?
Sir konsi book se reference liye ho
I took from multiple sources and compiled. No single book.
Nice
From Bangladesh 🇧🇩💗, i am a student of BANGLADESH AGRICULTURAL UNIVERSITY, and i am studying DVM
Hello!
আমিও।
Please upload more lec sir
Working on new videos. Will be uploading soon.
Thnk
❤❤❤❤
🙏🙏🙏
Is he speaking english ??
I am speaking Hindi.
Wish it were in English 😢, can't understand the language
Coming videos in English. Stay tuned!
why do you guys speak local language only on an international platform. many people are watching. not only the people from your country
In English, many creators are already available. But I received so many suggestions and I will definitely try to consider your suggestion. Thank you!
@@BioXLDyou could at least add subtitles 💔
I understand even it's not my language 😂
Please speak English!😢
Thank you sir
Nice