Great video, my college biochem professor is making us watch this video for lab today! I'm glad you're getting exposure and views!! Great explanation and visuals.
@@animatedbiologywitharpan You made an SDS gel and you forgot to add SDS in your gel mix. You realized this after you loaded your protein on the gel and began running it. What will your results look like? How will the protein migrate in the gel in the absence of SDS?
Excelent video. But I think there is a small mistake when you talk about Glycine. Glycine pI is 6.06, so at pH=6.8 it will have a weak negative charge and not a positive charge. If it had a positive charge it would not run into the gel, but go the opposite way and exit from above.
Thanks for this comment, makes this video somewhat comprehendable... Cant believe I pay for a lecturer to direct me to videos like this that aren't even fully correct!!
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Such an informative and amazing video. Such visuals will definitely help the learners to grasp the topics easily. Much grateful! Hope to see such amazing content in the future as well. 😍
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Could you please say that how can we experimentally analyse whether a single band represents a single protein or two different proteins with same molecular weight.?
@@animatedbiologywitharpan hey thank you so much. Btw, apart from 2D , can we use western blotting rather with the help of antibodies specific to my desired protein?
Great video, my college biochem professor is making us watch this video for lab today! I'm glad you're getting exposure and views!! Great explanation and visuals.
Thanks man ...please share among friends and help me to reach big audience
@@animatedbiologywitharpan You made an SDS gel and you forgot to add SDS in your gel mix. You realized this after you loaded your protein on the gel and began running it. What will your results look like? How will the protein migrate in the gel in the absence of SDS?
Excelent video.
But I think there is a small mistake when you talk about Glycine. Glycine pI is 6.06, so at pH=6.8 it will have a weak negative charge and not a positive charge. If it had a positive charge it would not run into the gel, but go the opposite way and exit from above.
Thanks for this comment, makes this video somewhat comprehendable... Cant believe I pay for a lecturer to direct me to videos like this that aren't even fully correct!!
Amino acid pKa1 pKa2 pI
Glycine 2.34 9.60 5.97
@@MannansElearning2021 why does the pka carboxylic acid change per molecule?
Very well explained with lots of information; thank you sir.
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Such an informative and amazing video. Such visuals will definitely help the learners to grasp the topics easily. Much grateful! Hope to see such amazing content in the future as well. 😍
Please share among your friends and help me to reach big audience
Amazing..thanks a lot for wonderful animation😊😊
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Fabulous! Thanks a million.
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Great explanation
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May I ask why the ionic strength necessary in the conduct of SDS-PAGE?
Could you please say that how can we experimentally analyse whether a single band represents a single protein or two different proteins with same molecular weight.?
Spend only 4 minutes to get this answer ua-cam.com/video/FHvblg338Gg/v-deo.html
@@animatedbiologywitharpan hey thank you so much. Btw, apart from 2D , can we use western blotting rather with the help of antibodies specific to my desired protein?
Thank you. it helped a lot.
Please sharee
Among your friends
Thank you so much!
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Amazing! thank you.
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Thank you!
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Our professor introduced us to your Channel. 😊
@@nikitampmaurya wow ...so nice to hear that....please share with your friends and help me to reach out big audience.
Why specifically 5% is used in stacking gel
Because it has quite big pore size
Loved it
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⭐Amazing Video Sir!
If u don't mind, Can I translate this video to my native language to explain ?
with respective credits to You.
Thank You sir!
Okk
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@@animatedbiologywitharpan of course sir!
Thank you
@@ThiNKBiologyThiNKVISION will you be translate it to english?
@@landrew3847 it's already in English
It would have been better if you had put the references on each slides.
That could be easier for us to find more infos
Thanks for the feedback, I will incorporate this in future
thank you
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what are the applications of this technique?
It can separate proteins based on molecular weight. It's the first step to perform a western blot.
Lovely
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Why the protein becomes free?
唐峰 I did not get the question ....could you please elaborate
Toooo goooodddd😘
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I am also available at Unaccademy
R u bangladeshi?
No, Indian
too good
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You took 1 minute 16 seconds extra.
Sorry about that, i hope that the content was useful
@@animatedbiologywitharpan was joking very well explained