How to make a microfluidic device

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  • Опубліковано 24 гру 2024

КОМЕНТАРІ • 63

  • @CuriousMindCheng
    @CuriousMindCheng 2 роки тому +6

    The people who made this video and decided to make it freely available to the public are saints.

  • @KUMAR-bq4xn
    @KUMAR-bq4xn 4 роки тому +5

    Great video. Very detailed and even the small details are included. Thanks for your efforts

  • @benh5336
    @benh5336 5 років тому +31

    This is all well and good, but does it work as well as the shrinky dink method?

    • @_c_e_
      @_c_e_ 5 років тому +1

      I Agree. I am a noob but I think the shrinky dink method (KISS) is far easier to develop with in both time and complexity, is resistant to loses of resolution to channel widths, and, is less fraught with turmoil and danger from toxic chemicals. Superior in many other ways too! (I mean, just watch this video in full for the 101 step madness). Nonetheless, this is a very good video describing a complex set of methods to a simple problem. Kind of reminds me of the old etching days of tinkering with pcb's... Now, design in cad, send to china, jump in glee as your product returns from the tech capital of the world in a lovely cardboard box bubble wrapped and certified to the nearest thou of a micron; can totally see this moving there if it has not done so already. Very interesting field.

    • @mynameisthis1580
      @mynameisthis1580 4 роки тому

      With shrinky dink, you're freehand drawing structures onto the chip. With PDMS (method in the video), you're designing the structures in autocad. Autocad will give much more precision, and is more easily shareable, than freehand drawing. Secondly, changing features, such as 5um channels to 6um channels is much easier to do when you're talking about autocad.
      In terms of costs, PDMS chips cost about ~$1 per chip.

    • @Mutantcy1992
      @Mutantcy1992 3 роки тому +1

      @@_c_e_ hey if you're still into this you should check out the ESCARGO method, as it enables you to make channels in PDMS without all the procedure shown in this video. It uses 3D printed ABS.

  • @NicholasMoschos
    @NicholasMoschos 9 днів тому

    What epoxy did you use for sealing the needles?

  • @nefariouscoon
    @nefariouscoon 6 років тому +2

    Thanks for sharing your optimized parameters...it was indeed a great video!

  • @anaxprincessx
    @anaxprincessx 4 роки тому +1

    Very informative and coherently put. Thank you so much.

  • @deepakkukkar6956
    @deepakkukkar6956 3 роки тому

    Excellent video. Thanks for sharing the details of various optimization parameters and do's and don'ts.

  • @RajasPoorna
    @RajasPoorna 2 роки тому

    Thank you for the detailed tutorial! I am grateful!
    Now that I know how complicated this process is, I will try to find a different process to make my microfluidics :D

    • @Chrisreynolds0724199
      @Chrisreynolds0724199 Рік тому

      You can do a very similar process with UV resin used in 3d printers. Resolution is not as good, but the process is much easier. Spin coat wafer with resin and cure under UV to make bonding layer. Then spin coat again and put mask over and expose to UV for ~30 seconds. Rinse with ethanol to remove excess then do additional UV cure for ~30 min. Then you are done! Takes about 1 hour total time. The resolution you get with UV curable resin is not as good as photoresist, but we can get 100 um resolution about.

  • @amirsharif3913
    @amirsharif3913 3 роки тому

    thanks for sharing this great video. I enjoyed watching it.

  • @samia-123-c1b
    @samia-123-c1b 6 років тому +1

    thank you for sharing this guide with us !

  • @arafat877
    @arafat877 3 роки тому +1

    Congratulations from Morocco, you have done a great job, could you share with me the list of the machines (uv exposure, plasma, heater ...), chemical products you have used to make this microfluedic device please ? As in Morocco we don't have those things, so I should buy them from outside of Morocco, and if possible a PDF version of this video, thank you in advance.

  • @sanampudasaini3379
    @sanampudasaini3379 7 років тому +1

    Excellent video. Thank you for making and sharing the video... Hoping for more videos regarding fabrications, tips, tricks etc... Bonding wire, sputter electrodes and all...
    Many people find binding wire to be difficult.

  • @pierangelogobbo6786
    @pierangelogobbo6786 6 років тому

    Thanks for sharing your experience guys. Excellent video!

  • @물장난감
    @물장난감 4 роки тому

    Thank you for detail information👍

  • @ashishamlan
    @ashishamlan 3 роки тому

    hey, can i get the equipment list and its link to purchase, which are being used in this experiment?

  • @pashasayyad3239
    @pashasayyad3239 4 роки тому

    Thank you for this valuable information. I have one question.
    Can you please suggest how we can integrate substrate inside the flow cell?

  • @micaheller4345
    @micaheller4345 6 років тому +2

    What type of needles are those that you can easily attach the tubing to?

    • @Mutantcy1992
      @Mutantcy1992 3 роки тому

      A similar kind is the butterfly needle, which is made to be fed by tube. That would require you blunting the tip though.

  • @ronymurickan8976
    @ronymurickan8976 4 роки тому

    Wonderful.. Well explained

  • @ozgeerguder731
    @ozgeerguder731 4 роки тому

    Thank you very much, science guys!

  • @bd8502
    @bd8502 10 місяців тому

    Very thorough.

  • @sankargollu0911
    @sankargollu0911 4 роки тому

    Nice video. Thank you for sharing. How it is different from microfluidics flexdym?

  • @kdm8568
    @kdm8568 7 років тому

    This is very helpful video! thanks a lot

  • @aunraza3427
    @aunraza3427 5 років тому

    amazing. thanks for sharing it

  • @teodygumabat4488
    @teodygumabat4488 6 років тому

    Hi, thank you very much. Can the oxygen/air plasma be replaced with something er.. cheaper?

    • @PBGetson
      @PBGetson 5 років тому +2

      Check this out.
      Worlds Smallest Tesla Valve? - Shrinky Dink (Shrink Film) Microfluidics
      ua-cam.com/video/eNBg_1GPuH0/v-deo.html

    • @Zaniahiononzenbei
      @Zaniahiononzenbei 5 років тому +1

      @@PBGetson Thought Emporium is an absolutely amazing channel.
      I'm hoping he'll try and make masters with the Shrinky-Dinks, and then produce silicone versions.
      He has a love for plasma, so I imagine he'll show how to do oxygen plasma bonding later on. :)

    • @Chrisreynolds0724199
      @Chrisreynolds0724199 Рік тому +1

      Posting for others in case someone else has this question: You can use a bunsen burner flame to plasma clean the PDMS and then bond it to the slide. It is more difficult to do and you may get small amounts of carbon residue in your microfluidic, but it can be done by holding the PDMS close to the flame, but not so close it burns.

  • @harkamanghag6877
    @harkamanghag6877 5 років тому

    Good Job Guys.

  • @adetoyesealbert2093
    @adetoyesealbert2093 4 роки тому

    Thank you for sharing

  • @rohinshyam3659
    @rohinshyam3659 4 роки тому

    What is the name of the film used to create the photoresist Mask?

  • @hootintsai7023
    @hootintsai7023 4 роки тому

    thanks ,its very useful

  • @carmeladeluca1153
    @carmeladeluca1153 5 років тому

    really clear.. thanks!

  • @yiyangwu1446
    @yiyangwu1446 6 років тому +1

    Thank you for sharing, it really helps a lot :)

  • @Acute.
    @Acute. 4 місяці тому

    Thank you

  • @Mohamed.wael7
    @Mohamed.wael7 6 років тому

    Fantastic!

  • @eliokarim6551
    @eliokarim6551 6 років тому

    Greattt 👌👌

  • @mnquispe7142
    @mnquispe7142 Рік тому

    Thank you!!!

  • @makarand309
    @makarand309 4 роки тому

    Brilliant

  • @BigBang4821
    @BigBang4821 5 років тому

    cool vid!

  • @iurirobert9845
    @iurirobert9845 9 місяців тому

    good video, but i am new at microfluidic devices so i think is not a introduction video because a few things went over my head

  • @lmbt456
    @lmbt456 3 роки тому

    What are its application???

  • @ismailerdem2060
    @ismailerdem2060 5 років тому

    Why not sla 3d printer

    • @kieramcadams4103
      @kieramcadams4103 5 років тому +2

      3d printers are relatively low res

    • @maravalverde5814
      @maravalverde5814 5 років тому

      @@kieramcadams4103 The 3D anycubic Photon has a 25-100 micron resolution, and it's a fairly cheap one, I found a paper to develop a device to perform RPA and they used a Viper to make their molds

    • @yummytis
      @yummytis 5 років тому

      Mara Valverde may i know which paper?

    • @ThanhNguyen-xb9iu
      @ThanhNguyen-xb9iu 3 роки тому

      @@maravalverde5814 25-100 micron resolution is still too big for making microfluidic chip.

  • @CostSteam02
    @CostSteam02 3 роки тому

    how did this get in my recommended and why did i watch it at 2am how did i get here

  • @figure35
    @figure35 6 років тому

    Thanks

  • @tru_710
    @tru_710 4 місяці тому

    Where can I get the books, chemicals and equipment at a discount?

    • @tru_710
      @tru_710 4 місяці тому

      Make one device that can do everything from cooking food to spinning and vibrating things for science and sell it everywhere

  • @improbabile
    @improbabile 7 місяців тому +1

    Guariscimi tutto anche alla signorina Maria pascarelli ravaschieri fieschi del drago sono Biagio di balsamo e famiglia sono a roccapiemonte a via gargiulo vicino alla scuola elementare quella vecchia vieni avanti a benedirmi le 3 case in cui abitiamo noi di balsamo e famiglia

  • @sprucescience
    @sprucescience 3 роки тому

    Using a vacuum mixer is a great way to prevent bubbles from forming in the PDMS mixture. Check out this video
    ua-cam.com/video/zDMnDpES0ZQ/v-deo.html