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Queen Mary Microfluidics
Приєднався 10 кві 2017
Home of the Queen Mary Microfluidics Team!
How to make a microfluidic device
This is a simple guide to making a PDMS microfluidic device from start to finish. We wanted to include practical tips to enable you to make high quality devices, and save you a lot of time and effort. If only we knew these tips when we started our own project!
Hopefully this will clear up some confusions and acxt as a supplementary resource to the available papers and literature available on this process.
Thanks to the QM microfluidics team:
Iejaaz Hussain
Omar Siddiq
Deniz Ucan
Shuhala Ahad
Elliot Allen
Romeo Murisa
Supervisor:
Dr. Yi Sui
Big thanks to Qin Guo for her guidance and help through this process. We would not have been able to do this without her!
Please leave your comments and questions below!
Hopefully this will clear up some confusions and acxt as a supplementary resource to the available papers and literature available on this process.
Thanks to the QM microfluidics team:
Iejaaz Hussain
Omar Siddiq
Deniz Ucan
Shuhala Ahad
Elliot Allen
Romeo Murisa
Supervisor:
Dr. Yi Sui
Big thanks to Qin Guo for her guidance and help through this process. We would not have been able to do this without her!
Please leave your comments and questions below!
Переглядів: 84 648
Guariscimi tutto anche alla signorina Maria pascarelli ravaschieri fieschi del drago sono Biagio di balsamo e famiglia sono a roccapiemonte a via gargiulo vicino alla scuola elementare quella vecchia vieni avanti a benedirmi le 3 case in cui abitiamo noi di balsamo e famiglia
good video, but i am new at microfluidic devices so i think is not a introduction video because a few things went over my head
Very thorough.
Thank you!!!
Helpful video, I suggest this video, it may be helpful too. ua-cam.com/video/r-W7PUs6nGk/v-deo.html
Thank you for the detailed tutorial! I am grateful! Now that I know how complicated this process is, I will try to find a different process to make my microfluidics :D
You can do a very similar process with UV resin used in 3d printers. Resolution is not as good, but the process is much easier. Spin coat wafer with resin and cure under UV to make bonding layer. Then spin coat again and put mask over and expose to UV for ~30 seconds. Rinse with ethanol to remove excess then do additional UV cure for ~30 min. Then you are done! Takes about 1 hour total time. The resolution you get with UV curable resin is not as good as photoresist, but we can get 100 um resolution about.
The people who made this video and decided to make it freely available to the public are saints.
Congratulations from Morocco, you have done a great job, could you share with me the list of the machines (uv exposure, plasma, heater ...), chemical products you have used to make this microfluedic device please ? As in Morocco we don't have those things, so I should buy them from outside of Morocco, and if possible a PDF version of this video, thank you in advance.
how did this get in my recommended and why did i watch it at 2am how did i get here
hey, can i get the equipment list and its link to purchase, which are being used in this experiment?
What are its application???
Micro fluids mainly medical precision dosages
Excellent video. Thanks for sharing the details of various optimization parameters and do's and don'ts.
thanks for sharing this great video. I enjoyed watching it.
Using a vacuum mixer is a great way to prevent bubbles from forming in the PDMS mixture. Check out this video ua-cam.com/video/zDMnDpES0ZQ/v-deo.html
For silicon and other substrates for microfluid devices, visit order.universitywafer.com
Thank you for this valuable information. I have one question. Can you please suggest how we can integrate substrate inside the flow cell?
Brilliant
thanks ,its very useful
Thank you for sharing
Thank you very much, science guys!
Great video. Very detailed and even the small details are included. Thanks for your efforts
What is the name of the film used to create the photoresist Mask?
mylar tympan with ink
Nice video. Thank you for sharing. How it is different from microfluidics flexdym?
Thank you for detail information👍
Wonderful.. Well explained
Very informative and coherently put. Thank you so much.
amazing. thanks for sharing it
really clear.. thanks!
Why not sla 3d printer
3d printers are relatively low res
@@kieramcadams4103 The 3D anycubic Photon has a 25-100 micron resolution, and it's a fairly cheap one, I found a paper to develop a device to perform RPA and they used a Viper to make their molds
Mara Valverde may i know which paper?
@@maravalverde5814 25-100 micron resolution is still too big for making microfluidic chip.
cool vid!
This is all well and good, but does it work as well as the shrinky dink method?
I Agree. I am a noob but I think the shrinky dink method (KISS) is far easier to develop with in both time and complexity, is resistant to loses of resolution to channel widths, and, is less fraught with turmoil and danger from toxic chemicals. Superior in many other ways too! (I mean, just watch this video in full for the 101 step madness). Nonetheless, this is a very good video describing a complex set of methods to a simple problem. Kind of reminds me of the old etching days of tinkering with pcb's... Now, design in cad, send to china, jump in glee as your product returns from the tech capital of the world in a lovely cardboard box bubble wrapped and certified to the nearest thou of a micron; can totally see this moving there if it has not done so already. Very interesting field.
With shrinky dink, you're freehand drawing structures onto the chip. With PDMS (method in the video), you're designing the structures in autocad. Autocad will give much more precision, and is more easily shareable, than freehand drawing. Secondly, changing features, such as 5um channels to 6um channels is much easier to do when you're talking about autocad. In terms of costs, PDMS chips cost about ~$1 per chip.
@@_c_e_ hey if you're still into this you should check out the ESCARGO method, as it enables you to make channels in PDMS without all the procedure shown in this video. It uses 3D printed ABS.
Good Job Guys.
Hi, thank you very much. Can the oxygen/air plasma be replaced with something er.. cheaper?
Check this out. Worlds Smallest Tesla Valve? - Shrinky Dink (Shrink Film) Microfluidics ua-cam.com/video/eNBg_1GPuH0/v-deo.html
@@P-B-G_YT Thought Emporium is an absolutely amazing channel. I'm hoping he'll try and make masters with the Shrinky-Dinks, and then produce silicone versions. He has a love for plasma, so I imagine he'll show how to do oxygen plasma bonding later on. :)
Posting for others in case someone else has this question: You can use a bunsen burner flame to plasma clean the PDMS and then bond it to the slide. It is more difficult to do and you may get small amounts of carbon residue in your microfluidic, but it can be done by holding the PDMS close to the flame, but not so close it burns.
Greattt 👌👌
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Thanks
Thank you for sharing, it really helps a lot :)
yeh
thank you for sharing this guide with us !
What type of needles are those that you can easily attach the tubing to?
A similar kind is the butterfly needle, which is made to be fed by tube. That would require you blunting the tip though.
Fantastic!
Thanks for sharing your optimized parameters...it was indeed a great video!
Thanks for sharing your experience guys. Excellent video!
Excellent video. Thank you for making and sharing the video... Hoping for more videos regarding fabrications, tips, tricks etc... Bonding wire, sputter electrodes and all... Many people find binding wire to be difficult.
This is very helpful video! thanks a lot