DNA Sequencing By Sanger Method

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  • Опубліковано 8 лис 2020
  • This Video Explains DNA Sequencing - Sanger Method.
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КОМЕНТАРІ • 36

  • @Braindeadsoda
    @Braindeadsoda Рік тому +30

    It is safe to say that this is the clearest video about Sanger Method I've found on UA-cam by far, thanks for your work!

  • @PomegranateDroid
    @PomegranateDroid Рік тому +10

    Lady, you just rescued my Biochemistry grade. Much appreciation.

  • @thiagoamorim9161
    @thiagoamorim9161 7 місяців тому +2

    Omg, i love u, my apresentarion of Master's degree is tomorrow and I was confused on this topic. You save me

  • @ahmadabdullah2997
    @ahmadabdullah2997 2 місяці тому +2

    God bless you I watched 3 lectures but could not understand the last part you made it clear
    Thank you❤

  • @bryanstapleton311
    @bryanstapleton311 9 місяців тому

    Im not sure if Im thinking about this right, but basically, since we can differentiate between fragment sizes based on the electrophoresis (assuming the gel can differentiate sizes of fragments by just 1 base pair), we can determine a sequence simply by analyzing where each fragment migrated in each tube based on the ddNTP?
    If that is correct, then how do we confirm the starting base pair of the sequence? I get that it would be the smallest fragment on the gel, therefore the band that traveled furthest, but that band can be a fragment that has 1 dNTP before the ddNTP, so that wouldn't tell us the very 1st base pair of the sequence necessarily.
    So in other words, how do we know that the smallest fragment had a ddNTP at the first base pair after the primer?
    Im not sure if that question makes since. But great video anyway, really clear.

  • @waterswater6544
    @waterswater6544 2 роки тому +3

    Needed this last year): but thank u for finally understanding this😍😍😍

  • @jackwining1928
    @jackwining1928 2 роки тому +3

    beautiful video, I'm currently doing my masters in Clinical immunology and was stuck on Sanger DNA method. THANK YOU

  • @demianashenouda5590
    @demianashenouda5590 6 місяців тому +2

    Thank you thank you. This was very easy to understand.

  • @mohammedal-hammadi5085
    @mohammedal-hammadi5085 3 роки тому +2

    Thank you for the informative videos

  • @user-cp1kz4gp9k
    @user-cp1kz4gp9k 2 місяці тому +2

    To the point video ever found

  • @raanamangrio322
    @raanamangrio322 4 місяці тому +2

    Best explained

  • @fanfan9768
    @fanfan9768 11 місяців тому

    How to calculate the number of fragments with different lengths? From short to long are N (Number of DNA template) x p (Termination probability), N x (1-p)*p ... ? Is it correct? why the band in the real gel is not like this?

  • @mehmethanarslan8829
    @mehmethanarslan8829 Рік тому +1

    Thank you thank you thank uuuuu

  • @ruvimbomahewu3925
    @ruvimbomahewu3925 2 місяці тому +1

    Thank you

  • @dusk0135
    @dusk0135 Рік тому +1

    Thanks!!!! 🤍🤍🤍🤍🤍💗

  • @spiritedzee1158
    @spiritedzee1158 4 місяці тому +1

    My Goly, I get it!

  • @khalidaamir2046
    @khalidaamir2046 3 роки тому +2

    Very nice

  • @coldsparks1662
    @coldsparks1662 2 місяці тому

    3:13 "when I added all those things in it" 😭😭 what things ?? please avoid saying vague THINGS like that . throughout the video, there were a couple vague words you used like that. Otherwise, the topic would have been clear .