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How to subculture (passage) primary cells
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- Опубліковано 26 сер 2012
- Learn more at www.lifetechnologies.com/prima...
This video shows you the tips and tricks that Life Technologies' experts use to successfully subculture any vial of Gibco® primary cells, and watching it will help you get the most from every vial of primary cells you purchase from us.
How long would Astrocytes need to be incubated at room temperature after receiving 3mL of Trypsin?
Thanks for your question Elizabeth. Please reach out to our technical support team at thermofisher.com/askaquestion so they can assist you. Thank you!
In this video. while gently mixing media with cells, bubbles were being created. Can these bubbles create adverse affects ?
From my knowledge sometimes yes, especially when cells are in a fragile state like after thawing from cryopreservation. I was always advised to minimize bubbles formed.
How can we release total amount of media from micro pipetting in 96 well plate... We we press to 1st stop it delivers only 90%... Remaining little liquid will stay in pipette tip if we push to 2nd stop and release air bubbles will form. . how to avoid the issue
Hi, this issue can only be overcome with careful and slow pipetting, and removing the tip from the solution during the second press before bubble is formed. One other method you can use if you plan to immediately discard the tip each time after using is to double press while taking liquid into the pipette tip, and pressing only once to release. But this method is less reliable in terms of the volume used, especially for viscous liquids.
Is it possible to know if the content of the pellets from calf blood are used in the final product of FOETAL BOVINE SERUM ( FBS) or the pellets are removed during coagulation to remove fibrinogen ?
They are removed during production of the serum. The serum does not have cells.
In the production of Fetal Bovine Serum (FBS), the content of the pellets from calf blood, which primarily consist of red blood cells (RBCs) and white blood cells (WBCs), are not used in the final FBS product.
Additionally, the majority of fibrinogen is removed during clotting and becomes part of the discarded pellet, trace amounts may remain in the serum. These trace amounts are generally not a concern for most cell culture applications.
However, if your cell culture experiments require absolute minimal fibrinogen content, you can opt for FBS that has undergone additional processing steps to further deplete fibrinogen. These options are often labeled as "low-fibrinogen FBS."
@@kosheeka many thanks for this complete info!
2:56 - How can 2.500 cells/cm^2 be equal to 125 cells/mL when 5,000 cells/cm^2 equals 25,000 cells/cm^2? Is that a typo?
Is it possible to know if the content of the pellets from calf blood are used in the final product of FOETAL BOVINE SERUM ( FBS) or the pellets are removed during coagulation to remove fibrinogen ?
Question: Why we just do not empty the flask by turning it upside down in place of media aspiration ? What could possibly go wrong ?
It increases risk of contamination, you can do it but it sometimes leaves media/liquid at the neck and rim of the flask. Some labs do do that though, it all depends on how confident you are with your skills, lab environment and incubators' sterility.
@@psi9899 thanks for answer
how you keep your lids, downward or upward
Keep the lids facing downward whenever you're not actively manipulating the cells. This minimizes the exposed surface area and reduces the risk of airborne contaminants entering the culture.
PLease somebody explain the formula from the minutes 2:53 . It is unclear : 2500 cells/cm2 = 1.25 x 102 cells/ ml
Hello, Thanks for your question. Can you please reach out to our technical support team at thermofisher.com/askaquestion. They would be the best team to assist. Thank you!
@@thermofisher they are the worst- because they do not want to respond.yes it is