Sub-culturing cells || Cell culture basics
Вставка
- Опубліковано 14 кві 2021
- Sub culturing (aka passaging), is the removal of the medium and transfer
of cells from a previous culture into fresh growth medium, a procedure that enables the further propagation of the cell line or cell strain.
The growth of cells in culture proceeds from the lag phase following seeding to the log phase, where the cells proliferate exponentially. When the cells in adherent cultures occupy all the available substrate and have no room left for expansion, or when the cells in suspension cultures exceed the capacity of the medium to support further growth, cell proliferation is greatly reduced or ceases entirely . To keep them at an optimal density for continued growth and to stimulate further proliferation, the culture has to divided and fresh medium supplied.
thank you for taking your time for making this video!!!!!!!!!!!! I am doing a internship in a lab where I am learning how to do cell culture but it is very intimidating at first,,, but this explained SO MANY CONFUSION.. THANK YOU FOR BEING YOU!!! wow
Glad to hear from you. If you like the explanation please share my channel link with your friends and help me to reach big audience.
Really good video! I have watched some before but this one provides the most clear information. Thank you!
Really glad to know it was useful. Please share my channel with friends. You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
well explained, thank you very much.
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
So much helpful and excellent presentation
well explained!! a surplus knowledge need to know.. thanks a lot.......😍
Well-explained, thanks 😊
Thanks alot for this! You are a blessing for biotechnology and microbiology students!
Please share my channel link with your friends and help me to reach big audience. 🙏
@@animatedbiologywitharpan yes I will definitely! Looking forward for more such Amazing videos
@@animatedbiologywitharpan yes I will always ✨
Thankyou for such informative video!
Please share my channel link with your friends and help me to reach big audiance
Thank uhh fr such a great explanation
Please share with your friends
Thanks for these topics, as ur video's helps in clear understanding off topics❤️❤️
More videos would be uploaded soon.....stay tuned
Waiting for u on unacademy
@@cartikey4687 due to covid lock down in Mumbai I might relocate to my home town and then be active again
Good to know that you guys like my classes.
@@animatedbiologywitharpan just trying to develop scientific approach like u...love u bro so much❤️
Loved it.. good understanding
Please share my channel link with your friends and help me to reach big audiance namaste 🙏
Nice presentation thank u , so helpful ..upload more videos ..pls
Check out the cell and tissue culture playlist
ua-cam.com/play/PLKtiwIJ8Q7rpNrZRxzBgoH2Q6oz4bJwg2.html
Please share my channel link with your friends and help me to reach big audience .
how to know the split ratio that I need to use to seed the cells?
You are awesome !
Please share my channel link in your college group
Thanks!
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
Hello,thanks for your amazing video.It's really useful in my experiment.But can you teach us about MTT assay this experiment?
Thanks for your suggestion....I will upload a video on MTT assay soon.
Do you use BioRender? Nice video man best of luck.
Please share my channel link with your friends and help me to reach big audience
thankyou sir
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
Nice vdo. 👌👌
Are you on unacademy??
Yes I am there
at what rpm do we centrifuge
200-300 rpm is good to precipitate cells
Sir primary secondary sub culture ki video khaaan hain ? Ye tu btain ..bht aala parha rai ap ...
Cell culture playlist ua-cam.com/play/PLKtiwIJ8Q7rpNrZRxzBgoH2Q6oz4bJwg2.html
Aala parha matlab ? Mai samjha nahi
Sir aala ka matlab hai bht acha...concepts clear ho gai .if i started listening u one day i will become microbiology expert 🥰🥰🥰🥰
Really nice to hear that from you. Please share my channel link with your friends
god bless indians
Please share my channel link with your friends and help me to reach big audiance
🙏🏻🙏🏻🙏🏻😊😊😊🥹🥹🙏🙏🙏
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
ا
Follow me on instagram and facebook
My social media handle is @animatedbiologywitharpan You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me