The tool used in this video at 1:21 is the plunger of a sterile disposable syringe. If you have any more questions, feel free to email us at techsupport@stemcell.com.
Hi, it should be possible to apply the protocol to individual brain regions for downstream microglia isolation but we have not tested it. It may require some optimization in terms of the amount of digestion medium per starting tissue, since it is currently optimized as 1 mL of digestion medium per whole brain of an adult mouse (as per the Product Information Sheet for Catalog #18970 EasySep™ Mouse CD11b Positive Selection Kit II: cdn.stemcell.com/media/files/pis/10000003693-PIS_02.pdf). If you have any more questions, feel free to email us at techsupport@stemcell.com.
The protocol uses EasySep™ Mouse CD11b Positive Selection Kit II (www.stemcell.com/easysep-mouse-cd11b-positive-selection-kit-ii.html ). The brain tissue preparation protocol is found in the 18970 PIS (cdn.stemcell.com/media/files/pis/10000003693-PIS_01.pdf ) but it was optimized for adult (>6 weeks) mouse brain samples. Unfortunately, we do not have extensive experience working with postnatal samples for this particular protocol. If you have any more questions, feel free to email us at techsupport@stemcell.com.
Hi Sivakumar, this video demonstrates mouse brain tissue preparation for the isolation of microglia using the EasySep™ Mouse CD11b Positive Selection Kit II (#18970). We do not have an equivalent protocol or product suggestion for human brain tissue, and do not endorse the use of this protocol for any application other than the one mentioned here. If you have any more questions, feel free to email us at techsupport@stemcell.com.
At 1:21, what is the tool being used to scrape the cell strainer?
The tool used in this video at 1:21 is the plunger of a sterile disposable syringe. If you have any more questions, feel free to email us at techsupport@stemcell.com.
Hi. Can this protocol be modified for brain regions instead of whole brain
Hi, it should be possible to apply the protocol to individual brain regions for downstream microglia isolation but we have not tested it. It may require some optimization in terms of the amount of digestion medium per starting tissue, since it is currently optimized as 1 mL of digestion medium per whole brain of an adult mouse (as per the Product Information Sheet for Catalog #18970 EasySep™ Mouse CD11b Positive Selection Kit II: cdn.stemcell.com/media/files/pis/10000003693-PIS_02.pdf). If you have any more questions, feel free to email us at techsupport@stemcell.com.
Great video! Thanks for sharing. Would this technique work for brain tissue of early postnatal mice?
The protocol uses EasySep™ Mouse CD11b Positive Selection Kit II (www.stemcell.com/easysep-mouse-cd11b-positive-selection-kit-ii.html ). The brain tissue preparation protocol is found in the 18970 PIS (cdn.stemcell.com/media/files/pis/10000003693-PIS_01.pdf ) but it was optimized for adult (>6 weeks) mouse brain samples. Unfortunately, we do not have extensive experience working with postnatal samples for this particular protocol. If you have any more questions, feel free to email us at techsupport@stemcell.com.
@@STEMCELLTechnologies The link is broken. Has it been combined into the NeuroCult Adult CNS kit?
Hi, please try the link again.
Is this technique will work for human also?
Hi Sivakumar, this video demonstrates mouse brain tissue preparation for the isolation of microglia using the EasySep™ Mouse CD11b Positive Selection Kit II (#18970). We do not have an equivalent protocol or product suggestion for human brain tissue, and do not endorse the use of this protocol for any application other than the one mentioned here. If you have any more questions, feel free to email us at techsupport@stemcell.com.