A New Pathway for Damaged Mitochondria - with Dr. Gustafsson

This week at Journal Club, Dr. Asa Gustafsson, Professor at the UC San Diego Skaggs School of Pharmacy and Pharmaceutical Science and Department of Pharmacology, School of Medicine presents her team's publication "Mitochondria are secreted in extracellular vesicles when lysosomal function is impaired" published in Nature Communications on 04 August, 2023. The study investigated how dysfunctional mitochondria are eliminated through large extracellular vesicles (EVs) via the endosomal pathway when lysosomal degradation is inhibited, especially in the context of cardiac health.
Link to full article:
www.nature.com/articles/s4146...
#journalclub #research #science #biology #mitophagy #mitochondria #ucsandiego #vincere
Dr. Gustafsson Profile & Lab Website:
Profile: pharmacy.ucsd.edu/faculty/gus... & profiles.ucsd.edu/asa.gustafsson
Lab Website: gustafssonlabucsd.org/team/
Chapters:
00:00-00:39 Introduction
00:40-02:42 Background
02:43-07:27 Results - Inhibiting Lysosomal Acidification Leads to Increased Release of Large and Small EVs from Cells and Hearts
07:28-12:07 Results - Rab7-deficent Cells Have Increased EV Secretion at Baseline
12:08-14:45 Sequestration of Mitochondria in CD81-positive Vesicles
14:46-19:08 Results - Rab27a Knockdown in Rab7-deficient Cells Abrogates Secretion of Large EVs Containing Mitochondria and Increases Susceptibility to Stress
19:09-22:58 Results - Characterization of Mice with Cardiac Specific Deletion of Rab7
22:59-26:26 Results - Enhanced Secretion of Large EVs Containing Mitochondria in Rab7-deficient Hearts
26:27-31:07 Results - Enhanced Secretion of Large EVs Containing Mitochondria in Aged and Lamp2-deficient Hearts
31:08- 34:55 Summary and Conclusion
34:56-45:10 Q&A