Dear sir, if in HPLC method gives dual wavelength detection, for IMP 3 at 210 nm & for IMP 1 & 2 at 230 nm. For purity, single imp and total imp no specific wavelength (210 nm or 230 nm) given. Then at which wavelength we can calculate purity, single imp & how we can calculate total imp? Pl guide sir
Excellent explanation 🌹
Good information
Really it's very helpful video for me
Nice explanation
Excellent Sir
Please prepare vedio of usp 621 recent revision
Why is detector wavelength considered, when it is not allowed to change?
simply best
Thanks a lot बड़का जी 🙂
Dear sir, if in HPLC method gives dual wavelength detection, for IMP 3 at 210 nm & for IMP 1 & 2 at 230 nm. For purity, single imp and total imp no specific wavelength (210 nm or 230 nm) given. Then at which wavelength we can calculate purity, single imp & how we can calculate total imp? Pl guide sir
don’t know
You have extract standard and impurity respective same wavelength then calculate
Sir, request for New change 621 USP
Why need to adjust these parameters means in which situations need to do this
because exact methods mentioned in monographs are not able to give results… so we need to optimise our method within mentioned limits of USP621
Is any chromatographic parameters adjustments are given in EP or BP. Kindly share as well as share general chapter no. for references.
okay
Partical size no increase this point not highlighted in USP chapter
Younot mention the mobile phase ratio adjustment for ternery mixture
sir If we adjust the chromatographic parameters then it affects in RT which is mentioned on pharmacopeia then how we can put a remark on it ?
ideally their should be a note point in your STP as well related adjustment whichever you are going to do.
thanks sir...❤️
Most welcome
New chang
Nice information
Thanks