Difference between C8 and C18 column। C8 Vs C18 column। HPLC reverses phase column

#EnglishExcel #HPLCcolumn #C8andC18column
Difference between C8 and C18 column.
These two types of column are very important and use in reverse phase chromatography. In this video I have discussed
#WhatisC8andC18column?
#DifferencebetweenC8andC18column
#ChemicalstructureofC8andC18column
#ReversephaseHPLCcolumn
#C8andC18column
#HPLCcolumn
#NoofcarbonatompresentinC8andC18column
#OctylchaininC8column
#OctadecylchaininC18column
#Hydrophobicity
#RetentiontimeinC8andC18column
#DensityofC8andC18column
#NatiureofstationaryphaseinC8andC18column
In this video i have differentiate the C8 and C18 column on the following 10 important parameter.........
1) Definition
2) No. of carbon atoms
3) Types of alkyl groups
4) Elution
5) Stationary phase
6) Retention time
7) Hydrophobicity
8) Degree of separation
9) Density
10) Type of separation
To follow my channel
/ @englishexcel2208
To follow our page on Face book
www.facebook.c...
To follow our Telegram channel
t.me/English_E...
If you like this video so please tap on the like button and don't forget to subscribe my channel #EnglishExcel
Don't forget to like share and subscribe.
Thanks for watching 😊

[@sujatadhyani6114]

Well explained.

[@atharhashmi5762]

Good work

[@maheshvishwakarma8828]

Thanks sir

[@Johar_Manbhum]

Verry nice

[@rahulr4220]

3:57 stationary phase

[@searchhere4797]

So retention time and separation depends on polarity of molecule in some cases retention time less for c8 column and some cases in C18 columns . It depends on mobile phase and analyte polarity

[@EnglishExcel2208]

Thanks for your valuable reply....

[@searchhere4797]

C8 column hydrophobicity lesser than C18 column , when a polar molecule will retain more time on C8 column than C18 column . So retention time less in C18 column than C8 column .

[@EnglishExcel2208]

Thank you

[@shibamdas915]

analyte will only move slower when it is non polar in C18. right??

[@marouaboudali2968]

Thank you 😍

[@EnglishExcel2208]

You are most welcome

[@alphacricketnews4546]

Thank you so much

[@msmahato8477]

Thanks....

[@Shubhampatil-bw1pj]

Dear sir
What is the role of active and deactivate OH group in C8 and C18

[@mubakhan8786]

which column is used for HbA2?

[@muhammedkashif105]

Nice

[@EnglishExcel2208]

Thank you so much...

[@Ambient_Scenes]

Can you use C18 or C8 columns be used to separate mixtures containing amine salts. I.e. will it be able to separate a mixture of for instance serotonin.HCl and dopamine.HCl ?

[@madugulasathish6361]

Tq sir good information

[@EnglishExcel2208]

You are most welcome
Thanks for watching...

[@shenubhanu8252]

Suprb bro tqs

[@EnglishExcel2208]

Thank you

[@marouaboudali2968]

Thank you 😍

[@dipuswapnu]

Thank you sir

[@EnglishExcel2208]

you r most welcome........

[@dipuswapnu]

@@EnglishExcel2208 sir exactly what is no.of theriotical plate in hplc either not less than 1500 or either NLT 2500? please inform me.🙏

[@EnglishExcel2208]

@@dipuswapnu minimum requirment is NLT 2000. If more than 2000 than it is very good bcoz peak sharpness becomes better when your theoritical plates are increased...............

[@dipuswapnu]

@@EnglishExcel2208 Thank you sir 🙏